Yazar "Dolak, Ibrahim" seçeneğine göre listele

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    A novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis
    (Elsevier Sci Ltd, 2020) Dolak, Ibrahim; Canpolat, Gurbet; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, Arzu
    Purification and analyzing of proteins is an essential means for understanding their function and diseases associated with their lack or defect. In this research, a new lanthanide-chelate based molecularly imprinted polymer (MIP) was synthesized for selective separation of Hemoglobin (Hb) from human serum in the presence of various interference molecules. The Hb-imprinted polymer was prepared by using complex functional monomer N-methacryloylamido antipyrine (MAAP)-Ce(III) and 2-Hydroxyethyl methacrylate (HEMA) in accordance of cryopolymerization techniques. The nonimprinted cryogel (NIP) was also prepared at same polymerization conditions in the absence of template Hb molecule. The effects of pH, initial Hb concentration, flow rate, temperature and ionic strength on the binding capacity of both imprinted and nonimprinted cryogels was investigated. The maximum binding capacity for the MIP column was found to be as 79.41 mg g(-1) dry cryogel, that is four times higher than the NIP column under the optimum conditions (pH 5.0, flow rate: 1.0 mL min(-1), T: 25 degrees C). Moreover, selectivity experiments were performed by using two interference proteins as myoglobin (Mb) and cytochrome c (Cyt-c) and the relative selectivity coefficients (k') for Hb/Mb and Hb/Cyt-c pairs were determined as 36.59 and 37.22, respectively.
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    Anoxybacillus sp. SO B1-Immobilized Amberlite XAD-16 for Solid-Phase Preconcentration of Cu(II) and Pb(II) and Their Determinations by Flame Atomic Absorption Spectrometry
    (Taylor & Francis Inc, 2015) Oral, Elif Varhan; Ozdemir, Sadin; Dolak, Ibrahim; Okumus, Veysi; Dundar, Abdurrahman; Ziyadanogullari, Berrin; Aksoy, Zehra
    A new method for the determination of Cu(II) and Pb(II) by flame atomic absorption spectrometry (FAAS) after preconcentrating on a column containing Anoxybacillus sp. SO B1-immobilized Amberlite XAD-16 was developed. The functional groups of Anoxybacillus sp. SO B1 immobilized on Amberlite XAD-16 were characterized in KBr tablets by Fourier transform infrared (FT-IR) spectrometry. Various parameters such as pH, amount of the adsorbent, eluent type and volume, and flow rate of the sample solution were studied. The optimum pH values of quantitative sorption for Cu(II) and Pb(II) were found to be pH 7.0 and 5.0 and Cu(II) and Pb(II) ions could be quantitatively eluted with 5.0ml of 1.0mol L-1 HCI and 10.0ml of 0.25mol L-1 HNO3, respectively. Recoveries of Cu(II) and Pb(II) were found to be 100.9 +/- 1.57% and 100.3 +/- 0.49% (N = 5), the limits of detection of Cu(II) and Pb(II) in the determination by FAAS (3s, N = 10) were found to be 0.8 and 1.6 mu g L-1, respectively. The proposed enrichment method was applied for metal ion determination from water samples such as two parts of Tigris River water in Diyarbakir and Elazig, Lake of Hazar in Elazig, and tap water in Diyarbakir. Furthermore, the accuracy of the proposed method was verified by studying the analytical recovery and by analyzing certified reference material (NCS-DC 73350 leaves of poplar).
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    Conductive Polymer-Based Nanocomposites as Powerful Sorbents: Design, Preparation and Extraction Applications
    (Taylor & Francis Inc, 2022) Canpolat, Gurbet; Dolak, Ibrahim; Kecili, Rustem; Hussain, Chaudhery Ghazanfar; Amiri, Amirhassan; Hussain, Chaudhery Mustansar
    Conductive polymers as composite materials have been attracted tremendous attention due to their versatile and excellent features such as tunable conductivity, facile synthesis and fabrication, high chemical and thermal stability etc. These characteristics make them versatile and let them being used in numerous fields including microelectronics, optics and biosensors. Throughout the mentioned fields, conductive polymers particularly perform as effective sorbents. Although tremendous efforts have been put into this topic, to the best of our knowledge, a comprehensive up-to-date review on the applications of conductive polymers as efficient sorbents has not been reported. The main objective of this paper is to make a significant contribution to the recent literature toward the synthesis and extraction applications of conductive polymers as efficient sorbents.
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    Development of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator
    (Elsevier Sci Ltd, 2021) Canpolat, Gurbet; Dolak, Ibrahim; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, Arzu
    The recognition and detection of proteins has an important role in the fields of biochemistry and medicine in term of diagnosis and prognosis. In this study, molecularly imprinted cryogel (MIP) was synthesized for the selective recognition of Cytochrome-c (Cyt-c) by cryopolymerization techniques with the lanthanide-chelate approach using (MAAP)2-Ce(III) as complex functional monomer and Cyt-c as a template protein. The incorporation of template with monomer was characterized by near- infrared (Near-IR) spectroscopy. The binding capacity was optimized in accordance with numerous experimental parameters including pH, initial Cyt-c concentration, flow rate, temperature and ionic strength. As a result, the maximum binding capacity reached to 98.33 mg g-1 in a buffer system with a pH of 6.0. Beside of the excellent reusability performance of prepared MIP, it was successfully applied to separation of template Cyt-c in the existence of hemoglobin (Hb) and myoglobin (Mb) chosen as interfering proteins. The relative selectivity constants of the Cyt-imprinted polymer were determined as 13.84 for the Cyt/Mb pair and 16.05 for the Cyt/Hb pair. Consequently, a high selectivity, simple and cheap strategy for efficient separation of proteins was provided with this novel MIP cryogel.
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    Functionalized magnetic nanoparticles as powerful sorbents and stationary phases for the extraction and chromatographic applications
    (Elsevier Sci Ltd, 2021) Kecili, Rustem; Ghorbani-Bidkorbeh, Fatemeh; Dolak, Ibrahim; Canpolat, Gurbet; Karabork, Muharrem; Hussain, Chaudhery Mustansar
    Materials in nanodimensions such as magnetic nanoparticles exibit fascinating physico-chemical features (i.e. high surface area, very small size, superparamagnetism, active surface which can be successfully functionalized, low toxicity, etc). Because of these superior features, functionalized magnetic nanoparticles received increasing attention for tremendous amount of studies over the last years and a number of successful applications were published. This paper aims to present and highlight the latest progresses in the development of novel functionalized magnetic nanoparticles for the extraction and chromatographic applications. (C) 2021 Elsevier B.V. All rights reserved.
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    Metal chelate based site recognition of ceruloplasmin using molecularly imprinted polymer/cryogel system
    (Taylor & Francis Group, 2020) Dolak, Ibrahim; Canpolat, Gurbet; Ersöz, Arzu; Say, Rıdvan
    In the present study, N-Acetylneuraminic acid (NANA)-imprinted poly(2-hydroxyethyl methacrylate- N-methacryloyl-(L)-histidin-Cu(II)) p(HEMA-(MAH)2-Cu(II)) has been synthesized by radical polymerization for site recognition of ceruloplasmin. Prepared imprinted cryogel has been characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). When the binding capacity of NANA-imprinted cryogel (MIP) was compared with nonimprinted cryogel (NIP), it was found that MIP has higher adsorption capacity. The maximum amount of binding NANA has found as 83.20 mgg−1 at pH 7.0 with flow rate of 1 mLmin−1 at 25°C. Selectivity experiments for MIP and NIP cryogel have been carried out via the NANA/D-mannose and ceruloplasmin/immunoglobulin G (IgG) pair separately and the relative selectivity coefficient (k’) has found as 77.46 and 17.97, respectively. Applicable of the MIP in human serum has been studied and resulted successfully.
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    Removal and Preconcentration of Pb(II), Cr(III), Cr(VI) from the Aqueous Solution and Speciation of Cr(III)-Cr(VI) by Using Functionalized Amberlite XAD-16 Resin with Dithioethylenediamine
    (Asian Journal Of Chemistry, 2010) Dolak, Ibrahim; Tegin, Ibrahim; Guzel, Remziye; Ziyadanogullari, Recep
    Amberlit XAD-16 was functionalized using dithioethylenediamine as a ligand and characterized by elemental analysis and FTIR spectroscopy. The effects of contact time, pH, electrolyte and the concentration of eluent on separation and preconcentration of Pb(II), Cr(III) and Cr(VI) were studied. In addition, the batch capacity for each ion was determined under the optimum conditions. The preconcentration yields of Pb(II), Cr(III) and Cr(VI) were found to be 96.60 +/- 3.02, 102.20 +/- 3.00 and 100.64 +/- 2.24 %, respectively, with 500 times preconcentration factor under the optimum conditions. The speciation of Cr(III)-Cr(VI) was studied, it was determined that Cr(III) separated in 93.24 +/- 2.21 % yield from the solution containing Cr(III)-Cr(VI) by using Amberlit XAD-16-dithioethylenediamine and Cr(VI) remained in the solution at pH 4, while Cr(VI) separated in 101.43 +/- 3.09 % yield from the solution containing Cr(III)-Cr(VI) and Cr(III) remained in the solution. at pH 7.50 by using Amberlit XAD-16-dithioethylenediamine-Pb(II).
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    Removal and Preconcentration of Pb(II), V(V), Cr(VI) from the Aqueous Solution and Selective Separation of V(V)-Cr(VI) by Using Functionalized Amberlite XAD-16 Resin with Dithioethylenediamine
    (Asian Journal Of Chemistry, 2010) Dolak, Ibrahim; Tegin, Ibrahim; Guzel, Remziye; Ziyadanogullari, Recep
    Amberlit XAD-16 was functionalized using dithioethylenediamine as a ligand. and characterized by elemental analysis and FTIR spectroscopy. The effects of contact time, pH, electrolyte and the concentration of eluents on separation and preconcentration of Pb(II), Cr(VI) and V(V) were studied. In addition, the batch capacity for each ion was determined under the optimum conditions. The preconcentration yields of Pb(II), Cr(VI) and V(V) were found to be 96.60 +/- 3.02, 100.64 +/- 2.24 and 98.20 +/- 3.00 %, respectively, with 500 times preconcentration factor under the optimum conditions. The selective separation of Cr(VI)-V(V) was studied in terms of pH. It was determined that V(V) separated in 98.24 +/- 1.21 % yield from the solution containing Cr(VI)-V(V) and Cr(VI) remained in the solution at pH 2.5, while Cr(VI) separated in 101.43 +/- 3.09 % yield from the solution containing Cr(VI)-V(V) and V(V) remained in the solution at pH 8.5 by using Amberlit XAD-16-dithioethylenediamine -Pb(II).
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    Sample Preparation with Conductive Polymers
    (American Chemical Society, 2022) Canpolat, Gurbet; Dolak, Ibrahim; Hussain, Chaudhery Ghazanfar; Keçili, Rüstem; Hussain, Chaudhery Mustansar
    This chapter presents and highlights the latest progress in the synthesis andapplications of conductive polymers and their nanocomposites as powerfulsorbents in different sample preparation techniques including solid-phaseextraction, solid-phase microextraction, dispersive solid-phase extraction, andmagnetic solid-phase extraction. The review covers the extraction of heavy metalsand organic analytes from various food, environmental, and biological samplessuch as water, urine, and blood. In addition, electrochemical electrodes based onCPs and their composites are also summarized. © 2022 American Chemical Society. All rights reserved.
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    Smartphone-based optical and electrochemical sensing
    (Elsevier, 2021) Keçili, Rüstem; Ghorbani-Bidkorbeh, Fatemeh; Dolak, Ibrahim; Canpolat, Gurbet; Hussain, Chaudhery Mustansar
    Smartphones are emerging as potential replacements for the instrumentation involved in performing various sensing applications such as fast diagnostic tests. This is fueled in part by the gradual increase of billions of smartphone users (~ 3.5 billion users in 2020) even in low-income countries. In this chapter, the latest advancements on the design, development and successful applications of smartphone-based optical (colorimetric, fluorescence, chemiluminescence etc.) and electrochemical sensor platforms was highlighted and overviewed. It starts with the sifnigifance and superior features of the smartphones in the introduction part. In the next sections, optical sensing and electrochemical sensing based on smartphone technology and various important examples from the literature are demonstrated. © 2021 Elsevier Inc. All rights reserved.