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Öğe Bitki Gelişimini Tetikleyen Rizobakterilerin Uygulandığı Buğdayda (Triticum aestivum L.) Kuraklık Stresi ileİlişkili Bazı Genlerin İfade Seviyesinin Ölçülmesi(2021) İnal, Behçet; Bektaş, Harun; Mirzapour, Mohsen; Altıntaş, Serdar; Çığ, Fatih; Cengiz, Mustafa; Sonkurt, MehmetEkmeklik buğday (Triticum aestivum L.) temel besin kaynağı olması ve tüm dünyada üretimi yapılabilen bir ürünolması nedeniyle sürdürülebilir tarım açısından en önemli bitki türlerinden biridir. Buğday bitkisinin genetik yapı olarakmısır, çeltik ve diğer tüm tarımsal ürünlerden daha kompleks bir yapıya sahip olması bu türün ıslahını zor ve uzun zamanalan bir süreç haline getirmektedir. Diğer taraftan verim değerlerinin istenilen noktalara getirilebilmesi buğdayın çevreselfaktörlere verdiği tepkilerin anlaşılması ile mümkün olabilmektedir. Bu çalışmada da buğday ıslahında en sık karşılaşılansorunlardan kuraklık ve hastalıklara karşı direncin ACC deaminaz etkisi gösteren PGPB (Plant Growth Promoting Bacteria)ile ilişkisi incelenmiştir. Çalışmamızda ACC deaminaz sentezleyen bakterilerin iki farklı ekmeklik buğday çeşidinde (Gerek79, Bezostaja 1) ve kuraklık koşullarındaki etkisi incelenmiştir. Çalışma ile ACC deaminaz etkisi ile kuraklığa karşıdayanıklılık mekanizmasında rol alan bazı transkripsiyon faktörlerin ifade seviyeleri q-RT PCR ile ölçülmüştür. Ayrıca heriki buğday genotipinde glutatyon redüktaz seviyesi ölçülerek genler ile olan ilişkisi ortaya konulmuştur. Çalışma sonucundaelde edilen veriler değişen etkinlik derecesine sahip olmakla birlikte PGPB bakterilerinin kuraklık stresinin olumsuzetkilerini azaltıcı etkiye sahip olduğu bulunmuştur.Öğe Effect of Grain Size on The Root System Architecture of Bread Wheat (Triticum aestivum L.)(2020) Bektaş, Harun; Waınes, John GilesThe aim of this study was to investigate the role of grain size on seedling root architecture. Ten different breadwheat cultivars were selected to examine the effect of grain size on primary root traits under controlled conditions. Seminalroot traits were tested with germination papers at the growth stage 1. Significant differences between seminal root number,total seminal root length, longest root length, and root growth angle were observed among 10 cultivars. The seminal rootnumber per plant was found to be 3.93 in the large, 3.71 in the medium and 3.20 in the small grain groups. Similar rankingsin the seminal root length were observed, while root growth angle did not follow the same trend. The study suggested that thecultivar Atay 85 with superior primary root traits can be an advantage, especially in regions where plants achieve an advantageat a deep soil water level, under water stress at early growth stages.Öğe Mitigation of Drought Stress in Wheat by Bio-priming by PGPB Containing ACC Deaminase Activity(2022) Çığ, Fatih; Erman, Murat; İnal, Behçet; Bektaş, Harun; Sonkurt, Mehmet; Mirzapour, Mohsen; Ceritoğlu, MustafaOut of stress management strategies used for drought, inoculation of plant growth-promoting bacteria holds a major position due to sustainable, low-cost, and versatile properties. The plant growth-promoting bacteria, particularly containing 1-aminocyclopropane-1-carboxylic acid deaminase activity, have a critical location since they restrict ethylene synthesis under stress conditions thereby improving stress tolerance index. In this experiment, seeds of two wheat cul- tivars were primed with three bacterial strains and seedlings were grown under stress and non- stress conditions. The study was laid out in completely randomized factorial design with three replications. While plant growth achieved top performance with synthetic fertilizer in 80% of field capacity, increasing drought stress restricted the efficiency of synthetic fertilizer. In contrast, plant growth-promoting bacteria-priming promoted plant growth and dry matter accumulation under optimum and drought conditions. Increase of dry matter accumulation in treatments as con- trol plants varied between 17.1% and 57.1% under 80% of field capacity while it changed between 0.2% and 35.1% under drought conditions. TV126C and TV24C induced stress tolerance index in sensitive and tolerant cultivars under drought and optimum conditions. In conclusion, it is con- sidered that bio-priming with plant growth-promoting bacteria involving 1-aminocyclopropane- 1-carboxylic acid deaminase enzyme activity might be an effective and sustainable management strategy to drought stress in wheat cultivation.Öğe Root System Interactions of Common Vetch (Vicia sativa L.) and Triticale (Xtriticosecale Wittmack) Under Intercropping Conditions(2021) Açıkbaş, Semih; Özyazıcı, Mehmet Arif; Bektaş, HarunIntercropping, mixed cropping, and rotation are commonly applied and some of the oldest cropproduction techniques to improve soil structure and nitrogen status. While rotation takes generally two-seasonwith one triticeae and legume, inter-and mixed cropping takes place within the same season. Among manyadvantages of legume inter-and mixed cropping, the most important one is the sustainable use of soilresources and reduced input cost. Numerous studies evaluated the effect of inter -and mixed cropping onabove-ground interactions, very few investigated below-ground interactions in these systems. This study aimedto investigate common vetch (Vicia sativa) x triticale (Xtriticosecale Wittmack) root system interactions at theseedling stage under intercropping conditions. The study was conducted with a semi-hydroponic plexiglasssystem. Five different ratios were used to test species level intercommunication and competition. The resultssuggest that, while triticale significantly affected the development of common vetch roots, common vetch didnot affect triticale. Triticale with a fibrous root system was more aggressive on root growth compared tocommon vetch. It was seen that the most vigorous root system development (for both species) was at doseswith higher legume ratios. Our results highlight the importance of below-ground interactions for the selectionof best ratios to gain maximum outcome from the intercropping systems.Öğe The Effect of Plant Growth Promoting Rhizobacteria on Root Growth in Bread Wheat (Triticum aestivum L.)(2021) Bektaş, Harun; İnal, Behçet; Sonkurt, Mehmet; Çığ, Fatih; Bektaş, YaseminBread wheat (Triticum aestivum L.) is the most produced cool-season cereal in the worldand meets about 20% of our daily caloric intake. Climate change negatively affects grain yield, it is,therefore, necessary to improve climate-resilient wheat crops. It is a known fact that subsoil parametersare not mostly included in the breeding selection criteria due to some technical limitations. For thisreason, it is essential to examine the root system, which has a fundamental role in drought tolerance,for morphological, anatomical, physiological, and architectural aspects, to understand the geneticmechanisms of these traits and to determine breeding strategies. In this study, the seeds of twodifferent bread wheat varieties were inoculated by three different plant growth-promotingrhizobacteria (PGPR), which synthesize the ACC deaminase enzyme. When the results were evaluated,significant differences were observed between varieties and bacterial applications for the total rootlength and root growth angle. As a result of the study, it was observed that all three bacteria specieshad a positive effect on root development. Brevibacillus choshinensis was the most effectiveinoculation on total root length in Gerek 79 (95.4 cm), while it was Arthrobacter agilis in Bezostaja 1(62.8 cm). We suggest that plant growth-promoting rhizobacteria have a positive effect on wheat rootdevelopment and a detailed analysis of this effect should be carried out with future studies.Öğe The Effect of Salt Stress on Root Development and Arch7tecture 7n Common Grasspea (Lathyrus sat*vus L.)(2021) Bektaş, HarunPlant root development and archRtecture are experRencRng a perRod of Rncreased Rnterest due to clRmate change and RncreasRng drought stress pressure. It Rs known that root has a 3-dRmensRonal and complex developmental system, just lRke above-ground organs. DetermRnatRon of abRotRc stress factors affectRng root archRtecture and development Rn forage legume specRes Rs essentRal for developRng abRotRc stress-tolerant cultRvars and sustaRnable agrRcultural productRon. In thRs study, Rt was aRmed to RnvestRgate the effect of 0- to 300 mM salt (NaCl) doses on early root development, root archRtecture, and endosperm usage ratRos Rn two dRfferent common grasspea cultRvars (Lathyrus sat9vus L.) wRth known basRc developmental dRfferences. As a result of the study performed wRth the modRfRed cRgar- roll technRque, Rt was observed that Rn general, 100 to 150 mM and above doses RnhRbRt root growth. The number and length of roots are affected parallel to each other. It was observed that the genotypRc dRfferences between the cultRvars for the measured parameters related to root development were also reflected Rn the results. DRfferent cultRvars had dRfferent levels of tolerance, and the total number and length of roots affect the salt stress RnhRbRtRon. As a result of the stress encountered Rn the seedlRng stage, Rt was observed that the seed endosperm usage ratRo decreased up to 4-fold. It has been observed that evaluatRon of salt stress tolerance at the seedlRng stage may shed lRght on genotypRc dRfferences for germRnatRon and early development speed, and maybe Rmportant for earlRness.