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  • Küçük Resim
    Öğe
    Heavy metals biosorption by submerged aquatic plant nasturtium officinale
    (Asian Journal of Chemistry, 2010) Okumuş, Veysi; Başaran, Davut; Onay, Ahmet
    In the present investigation an aquatic plant, Nasturtium officinale was examined for its biosorption ability of heavy metal ions. The results obtained from biosorption experiments were used to understand the driving forces that govern the interaction between metal ions and biosorbent. The experimental biosorption data were fitted to the Scatchard plot, Langmiur and Freundlich isotherms. According to the parameters of the Langmiur isotherm, the maximum biosorption capacities of Cd2+ and Co2+ were 0.56 and 0.49 mg/g, respectively. In view of Freundlich isotherms, the maximum biosorption capacities of Cd2+ and Co2+ were 2.23 mg/g for both of metal ions.
  • Küçük Resim
    Öğe
    In vitro propagation of Diyarbakır watermelons and comparison of direct seeded and transplanted watermelon
    (Turkish Journal of Biology, 2011) Okumuş, Veysi; Pirinç, Vedat; Onay, Ahmet; Başaran, Davut
    A rapid protocol using shoot tip explants for micropropagation of Diyarbakır watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] types Surme, Beyazkış and Karakış was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. Th e eff ects of the diff erent concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. Th e results confi rmed that a range of 0.5-1.0 mg/L of BA was almost equally eff ective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 genotypes studied in terms of the number of proliferated shoots and the average shoot length obtained. Th e highest percentage of rooting was achieved when medium supplemented with 1.0 mg/L of indole-3-butyric acid (IBA) was used for the 3 genotypes. Th e highest frequency of acclimatized plantlets for the 3 genotypes was 85%, 85%, and 90%, respectively, in sterile compost, when the shoots of Beyazkış, Karakış, and Surme were rooted in the IBA-supplemented rooting treatments. Th e Surme genotype produced a signifi cantly higher yield and mean fruit weight than the Beyazkış and Karakış genotypes in both direct-seeded and transplanted watermelons. Th e main soluble sugar of the experimental types was separated, identifi ed, and quantifi ed using high performance liquid chromatography (HPLC). Fructose was found to be the most abundant sugar and was highly detected in Beyazkış for the seeded watermelon and in Karakış for the transplanted watermelon. Th is demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakır watermelon for use in breeding lines.
  • [ X ]
    Öğe
    In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon
    (2011) Okumuş, Veysi; Pirinç, Vedat; Onay, Ahmet; Başaran, Davut
    Sürgün ucu eksplantları kullanılarak Diyarbakır karpuz tipleri Sürme, Beyazkış ve Karakış’ın mikroçoğaltımı için hızlı bir protokol geliştirilmiştir. İn vitro koşullarda çimlendirilmiş 5 günlük fi delerin sürgün uçları 3 haft a süreyle sürgün rejenerasyon ortamında kültüre alınmıştır. Farklı konsantrasyonlardaki benzil adenin (BA) ve karbonhidrat tiplerinin sürgün proliferasyonuna etkileri test edilmiştir. Sonuçlar her 3 genotipte de 0,5-1,0 mg/L aralığındaki BA ortamında sürgün uzunluklarının hemen hemen eşit olduğunu göstermiş ve BA ortamına sukroz eklenmesiyle ortalama sürgün sayısı ve sürgün uzunluğunun diğer karbonhidratlara göre daha yüksek olduğu tespit edilmiştir. Her 3 genotip için en iyi köklenme ortamının 1.0 mg/L IBA olduğu belirlenmiş ve IBA’da köklendirilen sürgünler Beyazkış ve Karakış için % 85, Sürme için % 90 başarıyla steril komposta aktarılmıştır. Sürme genotipinde hem doğrudan tohumdan hem de aktarılmış fi delerden elde edilen ürün verimi ve ortalama meyve ağırlığı, Beyazkış ve Karakış’tan daha yüksek bulunmuştur. Temel çözünür şekerler HPLC (yüksek performanslı sıvı kromatografi ) ile kantitatif olarak tespit edilmiştir. Beyazkış’ta tohumdan elde edilen ve Karakış’ta aktarılmış fideden elde edilen karpuzda, en çok bulunan şeker fruktoz olarak tespit edilmiştir. Bu sonuçlarla yüksek kaliteli Diyarbakır karpuzunun in vitro çoğaltılabileceği gösterilmiştir.
  • Küçük Resim
    Öğe
    Simultaneous removal of indomethacine, papaverine and allopurinol from aqueous solution by using submerged aquatic plant nasturtium officinale
    (Asian Journal of Chemistry, 2010) Okumuş, Veysi; Varhan, Elif; Başaran, Davut; Onay, Ahmet
    Simultaneous removal of indomethacine, papaverine and allopurinol from aqueous solution by using submerged aquatic plant Nasturtium officinale biomass in high performance thin layer chromatography (HPTLC) was studied. Optimum biosorption conditions were determined as a function of contact time, pH, removal capacity of the amount of biomass and initial dry concentration. Experiments were performed in batch conditions. Concentrations of the drugs in the remaining solutions were simultaneously analyzed by HPTLC. Langmuir and Freundlich models were applied to describe the biosorption isotherm of the drugs by aquatic plant Nasturtium officinale biomass. According to the results, optimum parameters were found as 2.0 g biomass, pH:5.0 and 60 min contact time. Obtained from plots of Langmuir and Freundlich adsorption models, the highest drug uptakes were calculated from Langmuir isotherm and found to be 43.10, 39.68 and 38.61 mg/g for indomethacine, papaverine and allopurinol, respectively.