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    Evaluation of various biochemical parameters in the serum and tissues of lambs with white muscle disease
    (Agricultural Research Communication Centre, 2021) Irak, Kivanç; Mert, Handan; Mert, Nihat; Ayşin, Nesrullah; Söğütlü, İnci Doğan; Comba, Arzu
    Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined. Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups. © 2021 Agricultural Research Communication Centre. All rights reserved.
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    Honamli Goats Breed in South of Turkey I- Serum Mineral Analysis
    (2020) Demir, Ayşe Özge; İrak, Kıvanç; Mert, Handan; Mert, Nihat; Ayşin, Nesrullah; Söğütlü, İnci
    The objectivity of this study was to investigate some blood minerals and parameters in Honamli goats reared under semi-intensive conditions. 90 goats (2-4 ages) in different three herds kept under similar manage mental conditions were chosen as research materials in Teke Border of Antalya Province. Serum Ca 6.786±0.206 mg/dL, P 4.094±0.173 mg/dL, Cl 111.105±0.582 mmol/L, K 4.519±0.066 mmol/L, Mg 2.161±0.05 mg/dL, Na 148.047±0.508 mmol/L, Fe 110.706±2.510 ?g/dL, UIBC 155.025±4.333 ?g/dL and Tp 7.055±0.120 g/dL were determined by Roche Diagnostics, Cobas 8000 modular analyser series, immunoassay. After then, total iron- binding capacity (TIBC), transferrin (Tf), transferrin saturation (TSAT) and Ca++ levels were calculated from each other using different biochemical formulas. Ratios between the some minerals were calculated and presented in the text. Also, very high significant differences (P<0.0001) were found between herds for P, Cl, TIBC and Tf. However, no significant differences were found for Ca, Na, TSAT, TP and Ca results. Coefficients of variation (CV) were within 2 % and 35 % for all outcome parameters. In Pearson correlation analysis, negative and different two significant values (P<0.01 and P<0.001) were calculated between Fe and unsaturated iron binding capacity (UIBC) for all analysed groups. Controversially, positive and very high significant correlations (P<0.0001) were detected between Cl and Na. The results obtained from this study could serve as reference values for Honamli goats breeding in Mediterranean Region of Turkey.
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    The Effects of Grape Seed Extract on The Some Enzymes and Metabolites in Diabetic Rats
    (2018) İrak, Kıvanç; Pala, Nihat Mert; Mert, Handan; Ayşin, Nesrullah
    In this study, the effects of Grape Seed Extract (GSE) on glucose, enzymatic activities of elastase and collagenase, and certain biochemical parameters, were investigated. 32 female Wistar albino rats were used in the process. The subjects were randomly divided into 4 groups (control, diabetic (DM), GSE, DM+GSE). A single dose of saline was injected intraperitoneally to the rats in the control group and a single dose of streptozotocin (STZ, 45 mg/kg) was administered intraperitoneally to the rats in the diabetic group. In the GSE group, GSE (0.6 ml/rat) was administered daily via intragastric tube for 20 days, and DM+GSE group received a single dose of STZ intraperitoneally along with 0.6 ml GSE/rat. After 72 hours, blood samples were taken from all the rats. Rats with a fasting blood glucose level of 250 mg/dL and more were considered to be diabetic. At the end of the experiment, blood samples were taken again and were sent for elastase, collagenase, glucose, urea, total protein, cholesterol, and AOPP analysis. Glucose levels in diabetic rats were 671 mg/dL, compared to the glucose level of 335 mg/dL in the DM+GSE group. Total protein levels decreased in the DM+GSE group; the levels of glucose, urea, cholesterol and ascorbic acid also demonstrated a significant difference (p<0.001). No significant change was detected in the AOPP levels. The activities of collagenase (p<0.01) and elastase (p<0.05) in the DM group had moderate increases with respect to the control. It can, therefore, be surmised that the GSE reduces glucose levels, inhibits production of urea, regulates renal function, prevents possible nephropathy, overcomes albuminuria and alleviates protein loss, increases vitamin C levels and minimally affects collagenase and elastase activities.