Yazar "Alkan, Feray" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • [ X ]
    Öğe
    Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection
    (2022) Hacıoğlu, İlke Karayel; Alkan, Feray; Yelken, Selda Duran
    Aim: Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory illness in cattle, along with numerous other viral agents. Although the prevalence of BAdV has been determined by serological studies conducted in our\rcountry, there are limited data on the detection of BAdVs in respiratory system\rsamples, and there is no study on their molecular characterization. The aim of\rthis study was to detect and characterize BAdVs of the Mastadenovirus genus.\rMaterials and Methods: In this study, a total of 64 nasal swabs and one\rlung sample from 65 cattle of different ages with respiratory system disease\rsymptoms were used. After the extraction of viral DNA, they were tested by\rPCR using primers targeting the hexon gene region and the expected size of\ramplicons were sequenced.\rResults: According to the PCR results, three samples were found positive and\rthe positivity rate was detected as 4.6%. Out of positive samples, two were\rable to be sequenced and one clustered in the BAdV-2 serotype while the other\rwas grouped in the BAdV-3 serotype.\rConclusion: In this study, it was revealed that BAdVs are contributing agents\rin respiratory system infection in our country. Also, this is the first study reporting the molecular characterization of BAdV-2 and BAdV-3 in calves with\rrespiratory system infection in Turkey.
  • [ X ]
    Öğe
    Isolation and genetic characterization of bovine ephemeral fever virus from epidemic-2020 in Turkey
    (Springer, 2021) Karayel-Hacioglu, Ilke; Yelken, Selda Duran; Vezir, Yaser; Unal, Nilay; Alkan, Feray
    Bovine ephemeral fever virus (BEFV) infection occurs seasonally in many tropical and subtropical regions of Africa, Asia (including the Middle East), and Australia while it is exotic in Europe. In this study, the epidemiology of BEFV infection in Turkey that bridges southeastern Europe and Asia, geographically, was investigated according to the comparison of the nucleotide sequences of the virus caused the last epidemic in 2020 with those of the strains previously detected in Turkey as well as BEFV strains from other countries. In the phylogenetic analysis, based on an alignment of full-length G gene sequences, BEFVs from epidemic-2020 were located in Middle Eastern lineage and appear to represent most closely related BEFVs from India-2018 and 2019. The findings will contribute to a better understanding of BEFV epidemiology in Turkey.
  • [ X ]
    Öğe
    Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Turkiye
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2022) Karayel-Hacioglu, Ilke; Duran-Yelken, Selda; Alkan, Feray
    Enteric diseases are considered an important factor affecting the livestock industry; however, there are limited data on viruses that cause enteric diseases in small ruminants in our country. In this study, we focused on enterovirus (EV), hunnivirus (HuV), and kobuvirus (KoV), which are members of the family of Picornaviridae. In order to provide further information on the prevalence and the molecular epidemiology of these viruses, a total of 73 fecal samples or gut contents from diarrheic sheep and goats were screened for EV, HuV, and KoV. For this purpose, RT-PCRs were performed by using the specific primers for each virus. The prevalence rate determined in the sampled population was 2.7% (2/73) for each virus. In detail, EVs (2/60, 3.3%) and HuVs (2/60, 3.3%) were each detected in only sheep samples while KoVs were identified in a goat sample (1/13, 7.6%) and a sheep sample (1/60, 1.6%). There was no evidence of coinfection with these viruses in the tested animals. According to the results of the molecular analyzes, our EVs were clustered in caprine/ovinespecific EV-G and HuV strains retrieved in this study were grouped along with the other caprine/ovine origin sequences in Hunnivirus A2 genotype. Moreover, it was observed that the detected KoVs clustered in distinct species: Aichivirus B and Aichivirus C. In conclusion, this study, which reported the detection of EVs and HuVs from sheep as well as KoVs from sheep and goats in our country, provides valuable data on the epidemiology and molecular characteristics of these viruses.
  • [ X ]
    Öğe
    Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2019) Karayel Hacioglu, Ilke; Coskun, Nuvit; Duran Yelken, Selda; Sevinc, Secil; Alkan, Feray
    Bovine respiratory disease (BRD) causes economic losses related to a reduction in weight gain of affected animals, veterinary treatment costs, death, etc. One of the important respiratory tract disease viruses is the bovine respiratory syncytial virus (BRSV). In this study, it is aimed to report the molecular characterization of detected BRSVs. Therefore, nasal samples from three calves in a herd seen severe respiratory disorders were examined for BRSV and other possible viral etiological agents by PCRs and the amplicons were sequenced. In the phylogenetic tree, BRSV circulating in this herd is clustered with the genetic subgroup III BRSVs deposited in GenBank from some other countries. This study on the molecular characterization of BRSV circulating in calves would contribute for future studies on the epidemiology of this infection and the development and/or choice the effective vaccines in Turkey.
  • [ X ]
    Öğe
    The genotype determination and molecular characterization of bovine leukemia virus in Turkey
    (Univ Zagreb Vet Faculty, 2021) Alkan, Feray; Karayel-Hacioglu, Ilke; Yelken, Selda Duran; Coskun, Nuvit
    Bovine leukemia virus (BLV) infection is widespread worldwide and causes significant economic losses in the livestock industry. In this study, to identify the genotypes and to investigate the molecular characterization of BLV strains, we analyzed 25 BLV strains from cattle housed in six herds located in several Turkish geographical regions. Phylogenetic analysis, based on a partial or full-length sequence of the env , gp51 gene of BLVs, showed that all Turkish BLVs belonged to genotype 1. The alignment of the deduced amino acid sequences demonstrated fourteen amino acid substitutions in different regions of the env gp51. This study updates our knowledge of BLV in Turkey. Further studies are needed to understand the molecular epidemiology and implement a comprehensive BLV control program in Turkey.