Yazar "Akkoyun, Hurrem Turan" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • [ X ]
    Öğe
    Ameliorative effects of astaxanthin against copper(II) ion-induced alteration of pentose phosphate pathway and antioxidant system enzymes in rats
    (Springer Heidelberg, 2021) Akkoyun, Mahire Bayramoglu; Temel, Yusuf; Bengu, Aydin Sukru; Akkoyun, Hurrem Turan
    Copper (Cu) is one of the toxic elements that cause environmental pollution. As a result of excessive accumulation of copper in the organism, it causes damage in various organs and tissues and hemolysis in erythrocytes. Astaxanthin (ATX) is a pigment belonging to the xanthophyll family, which is an oxygenated derivative of carotenoids. Thanks to its powerful antioxidant properties, ATX has an extraordinary potential to protect the organism against various diseases, especially cancer. The main objective of this study was to investigate the toxic effect of copper ions on the glucose 6-phosphate dehydrogenase (G6PD), 6-phospho-gluconate dehydrogenase (6PGD), glutathione reductase (GR), glutathione S-transferase (GST), and thioredoxin reductase (TrxR) enzymes and the role of astaxanthin in reducing this effect. In in vivo study, Wistar Albino male rats (n=28) were randomly divided into 4 groups: the control group, copper (Cu2+) group, astaxanthin (ATX) group, and copper + astaxanthin (Cu2++ATX) group. The results show that G6PD enzyme activity in Cu2+ group was strongly inhibited (p < 0.05), while in other groups, there were no significant effects compared to the control group (p > 0.05). 6PGD enzyme activity was significantly reduced in Cu2+ group compared to that in the control group (p < 0.05), and GR enzyme activity was lower in Cu2+ group compared to that in the control group (p < 0.05). Similarly, when GST enzyme activity was evaluated, a strong decrease was observed in the Cu2+ group compared to that in the control group (p < 0.05), while the enzyme activity in the Cu2++ATX group approached the control group (p > 0.05). When TrxR enzyme activity level was examined, a statistically significant decrease was observed in the Cu2+ and Cu2++ATX groups (p < 0.05), and the enzyme activity in the ATX group was found to be close to that in the control group. When in vitro results were evaluated, it was observed that copper ions inhibited G6PD enzyme purified from rat erythrocyte tissues with IC50=1.90 mu M value and Ki = 0.97 mu M +/- 0.082 value and the inhibition was non-competitive. From the results, it can be concluded that Cu2+ ions have an inhibitory effect on rat erythrocyte pentose phosphate pathway and antioxidant system enzymes both in vivo and in vitro, and astaxanthin reduces this effect.
  • [ X ]
    Öğe
    Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats
    (Wiley, 2017) Temel, Yusuf; Bengu, Aydin Sukru; Akkoyun, Hurrem Turan; Akkoyun, Mahire; Ciftci, Mehmet
    In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2,5-ADP-Sepharose 4B affinity gel. The effects of Ast and Al3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al3+ inhibited the enzyme activity noncompetitively (IC50 values; 0.679mM, K-i values 1.32mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al+Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group (P<0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al+Ast groups (P<0.05).
  • [ X ]
    Öğe
    Effect Of Astaxanthin On Rat Brains Against Oxidative Stress Induced By Cadmium:Biochemical, Histopathological Evaluation
    (2018) Akkoyun, Hurrem Turan; Bengü, Aydın Şükrü; Ulucan, Aykut; Akkoyun, Mahire Bayramoğlu; Ekin, Suat; Temel, Yusuf; Çiftçi, Mehmet
    Aim of this study is to evaluate protective impact of Astaxanthin (AST) on rats with experimentalbrain injury induced with Cadmium (Cd). 32 male Wistar albino rats were divided into four groups as Control,Cadmium, Astaxanthin (AST), Cadmium (Cd)+Astaxanthin (AST). Rat brain tissues were obtained at the endof 30th day. Malondialdehyde (MDA), glutathione (GSH) levels and superoxide dismutase (SOD) enzymeactivities were measured in brain homogenates and histopathological examination was performed. MDA levelswere improvement in cadmium administered group (p<0.01) as well as Cd+AST administered group (p<0.05)compared to control group. In addition a substantial reduction Cd+AST group was observed compared to cadmiumadministered group (p<0.01). GSH level shows a decrease in Cd and Cd+AST groups compared to control (p<0.05).SOD enzyme activity was found significantly lower in Cd and Cd+AST groups compared to control (p<0.01). Inaddition, increase of SOD in Cd+AST group compared to cadmium group was also found significant (p<0.05).Histopathological findings in the cerebral cortex and hippocampus were different between groups. In the controland AST administered groups, normal histological structure was observed in the brain, while severe lesions wereseen in the Cd administered group and in the Cd+AST group only mild degenerative lesions were observed.As a result, elevated MDA level due to Cd administration was attenuated with AST administration. Decreased GSHlevel and SOD enzyme activity due to Cd administration was increased with AST administration. In addition, ASTadministration decreased histopathological lesions. Consequently, it is thought that AST may be used for protectionagainst brain oxidative damage due to Cd.
  • [ X ]
    Öğe
    Effect of boric acid on some elemental levels on rat's liver and kidney tissues during mercury chloride exposure
    (C M B Assoc, 2018) Akkoyun, Hurrem Turan
    In this study, the effect of boric acid on the important trace elements copper (Cu), iron (Fe), zinc (Zn), manganese (Mn) and nickel (Ni) in liver and kidney tissue of rats treated with mercury chloride was investigated. Twenty-four male Wistar albino rats (weighing 200 +/- 300 g) were divided into 3 groups: Control (C), Mercury chloride (HgCl2), Mercury chloride (HgCl2) + boric acid (BA). Iron and copper were decreased whereas Mn, Zn and Ni levels were increased in liver tissue in Hg administered group compared to control. Cu (p<0.01) and Mn (p<0.001) levels were increased in Hg + BA administered group compared to Hg group. Renal tissue Cu (p<0.01), Mn and Zn levels were increased whereas Ni (p<0.05) and Fe levels were decreased in Hg administered group compared to control group. Cu (p<0.001) and Zn (p<0.05) content increased in Hg + BA group compared to control group. As a result, it is thought that boric acid may have an effect on important trace element levels such as copper (Cu), iron (Fe), zinc (Zn), manganese (Mn), nickel (Ni) in case of oxidative stress caused by mercury chloride.
  • [ X ]
    Öğe
    Effect of Carvacrol on Biochemical and Behavioral Parameters in Acute Floride Administration
    (Wiley, 2023) Abbak, Nigar; Gul, Asli San Dagli; Gormus, Mustafa; Akkoyun, Hurrem Turan; Akkoyun, Mahire Bayramoglu; Arihan, Okan
    [Abstract Not Available]
  • [ X ]
    Öğe
    Effects of Subacute Carvacrol Administration on Behavioral and Oxidative Stress Parameters During NaF Exposure
    (Int Soc Fluoride Research, 2024) Abbak, Nigar; Dagli Gul, Asli San; Gormus, Mustafa; Akkoyun, Hurrem Turan; Akkoyun, Mahire Bayramoglu; Arihan, Okan
    Purpose: Fluoride is an element found in the earth's crust thatleachesinto drinking waterfrom sources where the rocks contain fluoride-rich minerals.Excessive fluoride exposure can lead to complications in various tissues and metabolism, particularly affectingdental and skeletal tissuesalongside others. This study aimedto examine the impact of carvacrol (CAR), one of the main active compounds in thyme, on behavior and oxidative stress in sodium fluoride (NaF)exposure.In the current study, the Open Field Test (OFT), Elevated Plus Maze (EPM),and Barnes Maze testswere used to examine anxiety-like behaviors and spatial memory. Methods: Herein, 32 male Balb/C mice were divided into four groups:the Controlgroup, CARgroup:20 mg/kgCARintraperitoneally ( i.p.), NaF group: 50 ppm NaF (in tap water), and NaF+CAR group: 50 ppm NaF (in tap water) + CAR 20 mg/kg i.p. The treatments continued for 14 days. At the end of the protocol, the OFT, EPM test, and Barnes Maze test were conducted. Oxidative stress parameters[superoxide dismutase (SOD),catalase (CAT),malondialdehyde (MDA)]were examined in the brain, kidney, and liver tissues. Results: The oxidative stress data indicated significant changes caused by the NaF and CAR. In the liver and kidneys, the enzyme activity values of SOD and CAT decreased significantly in the NaF group compared to the control and CAR groups, while in the brain, the CAT enzyme activity significantly decreased in the NaF group compared to control and CAR groups (p<0.05). Moreover, the levels of MDA, an importantmarker of lipid peroxidation, were significantly higher in the liver and brain tissues of the NaF group compared to the control group (p<0.05).The behavioral study results indicated no significant difference in the EPMtest but showed an increase in the number of rearingbehaviorsin the NaF group compared to the NaF+CAR group in the OFT, and a decrease in the grooming time in the NaF group compared to the CAR group (p<0.05). Conclusions: In the brain, kidney, and liver tissues, NaF exerted oxidative stress,as seen via the examined parameters (CAT, MDA, and SOD), while the CAR seemed to have an ameliorative effect against NaF-induced oxidative stress. The administration of NaF did not induce significant anxiety;on the contrary,the concomitant administration of NaF and CAR causedincreased anxiety-like behaviors compared tothe lone NaF exposure.