An evaluation for the standardization of the Alliumcepa test as cytotoxicity and genotoxicity assay

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dc.contributor.authorBonciu, Elena
dc.contributor.authorFirbas, Peter
dc.contributor.authorFontanetti, Carmem
dc.contributor.authorWusheng, Jiang
dc.contributor.authorKaraismailoğlu, Mehmet Cengiz
dc.contributor.authorLiu, Donghua
dc.contributor.authorMenicucci, Felicia
dc.contributor.authorPesnya, Dimytry
dc.contributor.authorPopescu, Aurel
dc.contributor.authorRomanovsky, Anton
dc.contributor.authorSchiff, Silvia
dc.contributor.authorSlusarczyk, Joanna
dc.contributor.authorDe Souza, Cleiton
dc.contributor.authorSrivastava, Alka
dc.contributor.authorSutan, Anca
dc.contributor.authorPapini, Alessio
dc.date.accessioned2019-11-19T09:38:39Z
dc.date.available2019-11-19T09:38:39Z
dc.date.issued2018-09
dc.departmentBelirleneceken_US
dc.description.abstractA general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student’s t, and the nonparametric tests Kruskal–Wallis and Mann–Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.en_US
dc.description.provenanceSubmitted by Mehmet Cengiz KARAİSMAİLOĞLU (cengiz.karaismailoglu@siirt.edu.tr) on 2019-11-19T09:38:39Z No. of bitstreams: 1 A13.pdf: 2839232 bytes, checksum: cd78dd33dd0dd713002cdb7e76198bdc (MD5)en
dc.description.provenanceMade available in DSpace on 2019-11-19T09:38:39Z (GMT). No. of bitstreams: 1 A13.pdf: 2839232 bytes, checksum: cd78dd33dd0dd713002cdb7e76198bdc (MD5) Previous issue date: 2018-09en
dc.identifier.citationBonciu Elena,Firbas Peter,Fontanetti Carmem S,Wusheng Jiang,KARAİSMAİLOĞLU MEHMET CENGİZ,Liu Donghua,Menicucci Felicia,Pesnya Dmitry S,Popescu Aurel,Romanovsky Anton V,Schiff Silvia,?lusarczyk Joanna,de Souza Cleiton,Srivastava Alka,Sutan Anca,Papini Alessio (2018). An evaluation for the standardization of the Alliumcepa test as cytotoxicity and genotoxicity assay. Caryologia, 71(3), 191-209., Doi: 10.1080/00087114.2018.1503496en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1707
dc.language.isoenen_US
dc.publisherCaryologiaen_US
dc.relation.publicationcategoryUlusal Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz#KayıtKontrol#
dc.subjectResearch Subject Categories::NATURAL SCIENCESen_US
dc.titleAn evaluation for the standardization of the Alliumcepa test as cytotoxicity and genotoxicity assayen_US
dc.typeArticleen_US

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