Katı Faz Fermantasyon Tekniği ile Bacillus circulans ATCC 4516’dan Ekstrasellüler β- Galaktosidaz Üretimi
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Dosyalar
Tarih
2019
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info:eu-repo/semantics/openAccess
Özet
Although most microbial enzymes are produced by submerged
fermentation, the use of agricultural wastes as substrates in solid
state fermentation (SSF) makes the production of enzymes more
economical. The use of economic substrates is of great interest for
making the enzyme production process significantly cheaper for SFF.
The aim of this study was to investigate the effect of some
parameters on the production of β-galactosidase from Bacillus
circulans ATCC 4516 using rice bran as substrate in solid state
fermentation (SFF) method. Certain fermentation parameters
involving incubation time, incubation temperature, inoculum level
and initial pH were studied separately. Maximal amount of β-
galactosidase production was obtained at 35% inoculum level, at
initial pH of 7.5, at 37ºC over 48 h. In addition, various carbon and
nitrogen sources were added to fermentation medium and the effect
on β-galactosidase production was investigated. According to the
results, carbon and nitrogen sources which added to the environment
suppressed the enzyme production.
Açıklama
Anahtar Kelimeler
Bacillus circulans ATCC 4516 β-galactosidase Solid state fermentation Optimization
Kaynak
KSÜ Tarım ve Doğa Derg
WoS Q Değeri
Scopus Q Değeri
Cilt
22
Sayı
3
