Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes
Yükleniyor...
Tarih
2012-07-12
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
International Journal of Nanomedicine
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical properties of the cell culture environments were evaluated using contact angle and surface roughness measurements and compared. Astrocyte morphological responses, including filopodia, lamellipodia, and stress fiber formation, and stellation were imaged using atomic force microscopy and phalloidin staining for F-actin. Activation of the corresponding Rho GTPase regulators was investigated using immunolabeling with Cdc42, Rac1, and RhoA. Astrocytes cultured on the nanofibrillar scaffolds showed a unique response that included stellation, cell–cell interactions by stellate processes, and evidence of depression of RhoA. The results support the hypothesis that the extracellular environment can trigger preferential activation of members of the Rho GTPase family, with demonstrable morphological consequences for cerebral cortical astrocytes.
Açıklama
Anahtar Kelimeler
stellation, nanofiber, RhoA, atomic force microscopy
Kaynak
WoS Q DeÄŸeri
Scopus Q DeÄŸeri
Cilt
Sayı
Künye
Tiryaki, VM, Ayres, VM, Khan, AA, Ahmed, I, Shreiber, DI, Meiners, S. Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes. Int. J. Nanomedicine. 2012 7:3891-3905 (DOI: 10.2147/IJN.S32681) ISSN: 1178-2013.
