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Öğe Genome-wide discovery of underlying genetic factors associated with fresh and frozen-thawed semen traits in composite ram breeds exhibiting different cryotolerance.(Elsevier BV, 2025-01-08) Bülbül, Bülent; Doğan, Şükrü; Dayanikli, Cemal; Kirbaş, Mesut; Şengül, Ebru; Kal, Yavuz; Yaman, YalçınFewer studies investigate the effects of underlying genetic factors related to semen characteristics, significantly affecting sheep farm profitability. This study aimed to identify single nucleotide polymorphisms (SNP) and genomic regions associated with fresh and frozen-thawed semen traits in rams with low (Hasak) and high (Hasmer) cryotolerance. Semen collected from 11 (5 Hasak with low and 6 Hasmer with high cryotolerance) rams cryopreserved in 0.25 ml straws in the breeding season. Quality characteristics were determined in fresh, equilibrated, and frozen-thawed semen. A Genome-Wide Association Study (GWAS) was conducted to unveil the genetic structure that might be attributed to cryotolerance in low and high cryotoleranced rams. Fresh (regarding total and progressive motility) and equilibrated semen quality were similar in Hasak and Hasmer rams (p > 0.6). However, the freeze-thawing process had a more pronounced negative effect on ram semen traits in Hasak than in Hasmer (p < 0.05). GWAS revealed 27 SNPs correlated with post-thaw semen parameters. Moreover, network analyses revealed pathways related to sperm ion channels and their activities, providing insights into the intricate molecular mechanisms underlying sperm physiology and emphasizing their role in potentially impacting sperm cryotolerance. The functional significance of detected SNPs and the associated pathways require further exploration.Öğe Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Middle Anatolian Merino(Springer Science and Business Media LLC, 2024-12-19) Yalçın Yaman; Şükrü DOĞAN; Mesut KIRBAŞ; A. Taner ÖNALDI; Yavuz KALThis study explored the genetic basis of growth traits in Middle Anatolian Merino lambs using multi-locus genome-wide association study (GWAS) analyses. Descriptive statistics indicated moderate heritability (h² = 0.363) for birth weight (BW) and (h² = 0.309) for weaning weight (WW), both statistically significant (p < 0.001). Strong genetic correlations were observed between WW and BW (rG = 0.922) and WW and Kleiber ratio (KR, rG = 0.896), implying that simultaneous improvements may be possible through targeted selection. Five multi-locus methods (mrMLM, FASTmrMLM, pLARmEB, FASTmrEMMA, and ISIS EM-BLASSO) were used to identify the polygenic basis of the traits. For BW and WW, 20 and 18 significant SNPs (LOD ≥ 5) were detected, respectively, with some SNPs co-detected by multiple methods. In contrast, only 10 significant SNPs were identified for KR, all exclusively by the ISIS EM-BLASSO approach. Pathway analyses within ± 100 Kb of associated SNPs revealed genes and pathways influencing these traits, which could be leveraged in future breeding programs for enhanced growth performance. The identified SNPs, particularly those associated with BW and WW traits, could facilitate genomic selection if validated in larger and more diverse populations.Öğe Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Middle Anatolian Merino(Springer Science and Business Media LLC, 2024-12-19) Yalçın Yaman; Şükrü DOĞAN; Mesut KIRBAŞ; A. Taner ÖNALDI; Yavuz KALThis study explored the genetic basis of growth traits in Middle Anatolian Merino lambs using multi-locus genome-wide association study (GWAS) analyses. Descriptive statistics indicated moderate heritability (h² = 0.363) for birth weight (BW) and (h² = 0.309) for weaning weight (WW), both statistically significant (p < 0.001). Strong genetic correlations were observed between WW and BW (rG = 0.922) and WW and Kleiber ratio (KR, rG = 0.896), implying that simultaneous improvements may be possible through targeted selection. Five multi-locus methods (mrMLM, FASTmrMLM, pLARmEB, FASTmrEMMA, and ISIS EM-BLASSO) were used to identify the polygenic basis of the traits. For BW and WW, 20 and 18 significant SNPs (LOD ≥ 5) were detected, respectively, with some SNPs co-detected by multiple methods. In contrast, only 10 significant SNPs were identified for KR, all exclusively by the ISIS EM-BLASSO approach. Pathway analyses within ± 100 Kb of associated SNPs revealed genes and pathways influencing these traits, which could be leveraged in future breeding programs for enhanced growth performance. The identified SNPs, particularly those associated with BW and WW traits, could facilitate genomic selection if validated in larger and more diverse populations.Öğe Effects of feeding by hay, grass silage and corn silage on growth performance, rumen fluid and blood serum parameters in beef cattle(Revue de Medecıne Veterınaıre, 2016) Tufan, Tuncay; Arslan, Cavit; Önk, Kadir; Sarı, Mehmet; Tilki, MuammerThe study was conducted to determine the use of hay, grass silage, corn silage and grass silage+corn silage as roughage sources on growth performance, some rumen and blood serum parameters in beef cattle. A total of 24 Simmental beef cattle (12 male, 12 female), 9 or 10 months age, which were initially 142.4 kg live weight, were used in this study. The animals were divided into four treatment groups each consisting of 6 cattle (3 male, 3 female). All animals in the groups were fed with 2888 g for 0-21 d, 3813 g for 22-42 d and 5177 g for 43-63 d concentrated feed. In addition to the concentrated feed, one of the following roughage source hay, grass silage, corn silage or grass silage+corn silage (50:50) were given ad libitum to animals in each group. Animals were fed by group basis. At the 63 d of the study, rumen fluid and blood serum samples were taken from the animals. Final live weights of the animals did not affect. Average daily weight gains of the cattle in the overall study were no statistical differences among the groups. The roughage sources did not affect rumen fluid pH, NH3 N, acetic-, propionic- and butyric acid concentrations of beef cattle. The roughage sources did not also affect serum glucose, total protein and albumin concentration of animals. Consequently, it is concluded that mentioned roughage sources could be preferred as alone or grass silage+corn silage combination in feeding of beef cattle after economic analyses of these feedstuffs.Öğe Effects of Different Fattening Systems on Fattening Performance, Slaughter and Carcass Characteristics of Male Tuj Lambs [(Kafkas Universitesi Veteriner Fakultesi Dergisi, 2017) Önk, Kadir; Sarı, Mehmet; Aksoy, Yüksel; Tilki, Muammer; Tufan, Tuncay; Yılmaz, İsaThe purpose of this study were to determine effects of fattening systems on fattening performance (n=39) and slaughter and carcass characteristics (n=24) in male Tuj lambs. Three different fattening groups were formed as extensive (n=13), semi-intensive (n=13) and intensive (n=13). Lambs in the extensive group were grazed in pasture. In addition to pasture, concentrated feed was given to lambs in the semi-intensive group. High quality fodder and concentrated feed were given to those in the intensive group. The study was completed in 90 d. Final live weights of extensive, semi-intensive and intensive groups were 31.19, 41.22, and 40.56 kg (P<0.001), respectively, and for daily live weight gain were (DLWG) 117.52, 229.66, and 221.11 g (P<0.001), respectively. Feed conversion rates (FCR) were 3.05 and 5.16 respectively in the semi-intensive and intensive fattening. It was found that hot carcass weight was 13.41, 19.51, and 19.89 kg (P<0.001) in extensive, semi-intensive, and intensive fattening groups respectively. Hot carcass yield was 43.11, 46.95, and 49.77% (P<0.001) in extensive, semi-intensive, and intensive fattening groups respectively. Consequently; leg, foreleg, shoulder, neck, flank, and kidney percentages, and spleen and full stomach weights of lambs in the extensive group were higher than in semi-extensive and entensive groups. Although there was no statistical difference between semi-extensive and entensive groups in respect to fattening performance, slaugher weight, hot and cold carcass weights, entensive group was higher than the other groups in respect to hot and cold carcass yield.Öğe GDO’ların üretim amaçları ve hayvan beslemede kullanımı(Turkiye Klinikleri Animal Nutrition and Nutritional Diseases-Special Topics, 5(2), 20-31., 2019) Arslan, Cavit; Tufan, TuncayBu derlemede genetik olarak değiştirilmiş organizma (GDO)’lı ürünlerin yetiştirilme amaçları, besinsel içeriği, yemlerdeki DNA’nın stabilitesi, hayvan beslemede kullanımı, organizmadaki akibeti ve geleceğe yönelik değerlendirmeler hakkında bilgi verilmiştir.Öğe Effects of Terebinth (Pistacia terebinthus L.) Fruit Oil Supplementation to Diets on Fattening Performance, Carcass Characteristics, Blood Parameters and Breast Meat Fatty Acid Composition in Japanese Quails (Coturnix coturnix Japonica)(Kafkas Universitesi Veteriner Fakultesi Dergisi, 2017) Tufan, Tuncay; Arslan, Cavit; Daş, AydınThe objective of this study was to determine effects of terebinth fruit oil (TFO) supplementation to diet on growth performance, carcass characteristics, some blood parameters and composition of breast meat fatty acids in Japanese quails. Totally 240 unsexed daily Japanese quail chicks were assigned randomly to three treatment groups. Each group divided into 4 replicates, each containing 20 birds. A group was fed with basal starter diet for 1-21th days and grower diet for 22-42th days (Control). Treatment groups were also fed same diets additionally their ration added by 130 (Group A) or 260 mg/kg TFO (Group B) during the study. At 42 d of study, 20 quails (5 quails from each subgroup) from each group slaughtered for determination of carcass traits, blood parameters and fatty acid composition of breast meat. As a result of this study, final live weight of quails in Group B was found higher than Control and Group A (P<0.05). There were no differences on the average live weight gains of the birds among the groups. Average feed intake in Group A was found lower than Control and Group B on basis of whole study period (P<0.05). Average feed conversion ratio in Group A was found better than Control on basis of whole study period (P<0.05). Carcass weight in Group B was found higher than Group A (P<0.05). There were no differences on carcass yield and breast-, leg-, wing-, heart-, liver- and gizzard ratio to carcass weights of the birds in all groups. Serum total cholesterol, high density lipoprotein, aspartate amino transferase, alkaline phosphatase, total protein, albumin and globulin did not differ among the groups. There were no differences on composition of breast meat fatty acid profiles. In conclusion, due to supplementation of 260 mg/kg TFO enhanced live weight and carcassÖğe Effects of dietary addition of synbiotic on the performance, carcass traits, and serum parameters of Japanese quails(Revista Brasileira de Zootecnia, 2017) Tufan, Tuncay; Bolacalı, MemişThis study aimed to evaluate the effects of different levels of synbiotic (Syn) on the growth performance, carcass traits, and serum parameters of Japanese quails. A total of 1,500 (750 male and 750 female) Japanese quails were assigned to five treatments, including the control (basal diet) and basal diets with the addition of 0.1, 0.2, 0.3, and 0.4% Syn according to a completely randomized design. Each treatment in the male and female experiment groups consisted of five replicates, with 30 chicks for each replicate. The addition of Syn had no effect on body weight (BW), while the BW of female quails was higher than that of male quails at day 42. The addition of Syn reduced feed intake (FI) and improved the feed conversion ratio (FCR) (P<0.000). The daily body weight gain and FI were significantly higher in female than in male quails and Syn also improved the FCR in the overall experiment. The effect of the addition of Syn on carcass parameters (except for abdominal fat) was not determined. The addition of Syn decreased abdominal fat weight and percentage when compared with the control group. Male quails were found to have higher carcass yield, wing, neck, and other percentages than female quails. However, female quails were found to have higher breast, breast skin, abdominal fat, and visceral organ percentages. The addition of 0.1% Syn to quail ration reduced serum aspartate aminotransferase, high-density lipoprotein (HDL), total protien (TP), and albumin (ALB) levels at day 42 of the trial. The addition of Syn decreased the serum total cholesterol level. Gender was found to have an effect in that female quails were found to have higher total cholesterol, HDL, TP, and ALB levels than male quails. In both genders, the addition of Syn improved the BWG, FC, and FCR in the overall experiment, reduced abdominal fat, decreased the total cholesterol levels, improved the HDL levels, and increased the TP and ALB levels at day 42. The addition of 0.3 and/or 0.4% synbiotic has a positive effect on quails by improving performance and enhancing some serum lipids and protein parameters.Öğe Effect of management system and dietary yeast autolysate on the performance, slaughter and carcass characteristics of partridges (Alectoris chukar)(South African Journal of Animal Science, 2018) Bolacalı, Memiş; Küçük, Mürsel; Tufan, Tuncay; Aslan, LokmanThis study was conducted to determine the effects of management system (a floor housing (FH) versus a free-range housing (FRH) system) and dietary yeast autolysate (Saccharomyces cerevisiae) (1% versus 2% dietary supplement) on the performance, slaughter and carcass characteristics of partridges (Alectoris chukar). A total of 480 (240 for each management system) one-day-old male partridge chicks were randomly allocated to a control group and the two dietary groups per production system, each containing 80 chicks. Each dietary group was then divided into five replicate groups of 16 chicks. The study lasted 112 days. In terms of growth performance, partridges reared under FRH management system had a lower average daily live weight gain (ADG), a significantly higher feed intake (FI), and feed conversion ratio (FCR) compared to the control group. However, partridges fed diets with yeast autolysate had a significantly higher ADG, lower FI, and an improved FCR compared to the control group overall. The gizzard, leg, and wing percentages of the partridges reared in the FRH system were found to be significantly higher than those of partridges reared in the FH system, while the breast and abdominal fat percentages were found to be lower. Dietary supplementation with yeast autolysate significantly increased the cold carcass yield and breast percentage when compared to the control group, while wing and abdominal fat percentages were decreased. In general, best performance was observed in the dietary supplementation group of 1% yeast autolysate in terms of growth and carcass parameters. When considering the fact that partridges are gamebirds, that housing poultry in cage production systems has been prohibited in recent years and that there are positive effects associated with using yeast autolysate as a prebiotic, it is recommended that a free-range production system should be used for partridge breeding, with the addition of 1% yeast autolysate to the diet of the birds.Öğe Effects of chitosan oligosaccharides and L-carnitine individually or concurrent supplementation for diets on growth performance, carcass traits and serum composition of broiler chickens(Revue de Medecıne Veterınaıre, 2018) Arslan, Cavit; Tufan, TuncayThis study was conducted to determine effects of dietary indivudually or dually supplementation of chitosan oligosacchacarides (COS) and L-carnitine to diet on growth performance, carcass traits and serum compositions of broiler chickens. A total of 320-day old broiler chicks were used in the study, and the chicks were divided into the following 4 main groups: Basal diet (control); basal diet+100 mg/kg COS (COS); basal diet+100 mg/kg L-carnitine (carnitine); and basal diet+100 mg/kg COS+100 mg/kg L-carnitine (COS+carnitine). Additionally, each of the main groups was divided into 4 subgroups of 20 chickens each. The study lasted for d 42. There were no differences on body weight, feed intake and feed convertion of birds overall the study. There were no differences on slaughter body weight, carcass weight, carcass ratio and breast, leg and wing ratio to carcass weight. Abdominal fat percentage in COS, carnitine and COS+carnitine was lower than the control group. Liver relative weight in COS and control group was higher than carnitine and COS+carnitine group. Total cholesterol, triglycerides, HDL, LDL, VLDL, total protein and albumin concentrations were not differ among groups. In conclusion, COS, carnitine and COS+carnitine supplementation to broiler diets did not affect growth performance, carcass traits and serum parameters, while carnitine and COS+carnitine can be use due to decreased abdominal fat and liver ratio in broilers.Öğe Japon Bıldırcınlarının Rasyonlarına Çörek Otu (Nigella sativa L.) Tohumu veya Çörek Otu Yağı İlavesinin Besi Performansı, Karkas Özellikleri ve Bazı Kan Parametrelerine Etkisi(2015-01-22) TUFAN, TUNCAYThis study was conducted to determine effects of black cumin seeds or black cumin oil addition to quail rations on growth performance, carcass traits and some blood parameters. Totally 180 daily Japanese quail chicks were used. Chicks allocated four main groups each containing 45 chicks, and each main group divided into three subgroups each containing 15 chicks. A group was fed with basal starter diet for 1-21th days and grower diet for 22-35th days (Control). Other groups were fed same diets but their diet added by 0.1% oxytetracycline (Positive control: PC), 1% black cumin seed (BCS) or 0.1% black cumin oil (BCO). At last of study 15 chicks from each main group were slaughtered for determination of carcass traits and blood serum parameters. Final average live weight of quails in PC and BCS groups were significantly higher than C group. There were no differences on live weight gain, feed consumption and feed conversion ratio among the groups on basis of whole study period. Carcass weight was lower in C group than the other groups, while there were no differences on carcass yield, leg, breast and wing ratio among the groups. Serum cholesterol concentration in BCO group was significantly lower than C group but there were no differences on Ca, P, VLDL, HDL, LDL, ALT, ALP, albumin, globulin, total protein concentrations among the groups. In conclusion, it was established that, as being in antibiotic added groups, addition of 1% black cumin seed to quail diet enhanced live weight, and 0.1% black cumin oil had showed hypocholesterolemic affect.
