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    A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey
    (Veterinarni A Farmaceuticka Univerzita Brno, 2022) Celik, Burcak Aslan; Celik, Ozgur Yasar; Ayan, Adnan; Yilmaz, Ali Bilgin; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
    This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.
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    MOLECULAR DETECTION OF MYCOPLASMA HAEMOCANIS AND CANDIDATUS MYCOPLASMA HAEMATOPARVUM IN RHIPICEPHALUS SANGUINE US TICK SPECIES COLLECTED FROM DOGS IN ADANA PROVINCE OF TURKEY
    (Parlar Scientific Publications (P S P), 2021) Sababoglu, Ezgi; Ayanz, Adnan; Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Tekindal, Mustafa Agah; Akkaya, Hayrettin; Celik, Burcak Aslan
    Mlycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) arc hemo-tropic mycoplasma species known to be specific to dogs. Rhipicephalus sanguineus (sensu lam) ticks have been suggested to bc the main vector in transmission; but a clear relationship between tick infestation and the disease has yet to be established under field conditions. This study aimed to investigate the presence of Mhc and CMhp in R. sanguineus (s.1.) s.ticks that arc considered to be a possible vector for hemoplasma infections, Using the species -specific polymerase chain reaction (PCR) test, the presence of Mhc. and CMhp was investigated in total of 312 archived adult tick samples collected from 63 pet dogs kept in the municipal dog shelter of Adana province located in the Mediterranean region of Turkey. Although R. sanguineus (s.1.) ticks tested negative for Mhc, 2.56% (n = 8) were positive for CMhp. There was a statistically significant difference in the Mhc and CMhp detection rates in R. sanguineus (s.1.) ticks (2.56%; 95% CI: 0.81 - 4.31; P < 0.05). To our knowledge, this is the first study to reveal the presence of CAlhp in R. sanguineus (s.l.) ticks collected from dogs in Turkey.
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    MOLECULAR IDENTIFICATION OF EHRLICHIA CANIS IN RHIPICEPHALUS SANGUINEUS TICKS FROM SIIRT PROVINCE
    (Sciendo, 2021) celik, Burcak Aslan; Ayan, Adnan; Yilmaz, Ali Bilgin; celik, Ozgur Yasar; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
    This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.
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    Molecular Identification of Hepatozoon canis in Ticks from Dogs in Siirt, Turkey
    (Natl Information Documentation Centre, 2022) Celik, Burcak Aslan; Ayan, Adnan; Yilmaz, Ali Bilgin; Celik, Ozgur Yasar; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
    HEPATAZOON species are tick borne protozoan parasites classified in the Heptazoidae family, and they are closely related to hemosporinids and piroplasms. In this work, the 18S rRNA genetic section of Hepatozoon canispositive ticks removed from dogs was sequenced using the PCR technique. Ticks were collected from a total of 80 dogs in Siirt, Turkey.A total of 300 collected ticks were morphologically identified to the species level and all ticks identified as Rhipicephalussanguineus (s.l.). H. canis DNA was detected in 12 (%4) out of 300 in R. sanguineusticks by PCR. The phylogenetic tree created via comparison of amplified 18S rRNA region sequences of H. caniswith MT107097.1, MH595911.1, KT215377.1, KT 215376.1, KC 584780.1, KC 584777.1, KC 584775.1, and KC 584774.1. The results obtained will provide important reference material for both veterinary cliniciansand dog owners in terms of managing canine hepatozoonosis.
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    MOLECULAR INVESTIGATION OF SOME BACTERIA (COXİELLA BURNETİİ, MYCOPLASMA HAEMOCANİS, CANDİDATUS MYCOPLASMA HAEMATOPARVUM, WOLBACHİA) IN RHIPICEPHALUS SANGUINEUS TICKS IN SIIRT PROVINCE, TURKEY
    (Assiut University, Faculty of Veterinary Medicine, 2022) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Yilmaz, Ali Bilgin; Kilinç, Özlem Orunç; Şababoğlu, Ezgi; Ayan, Özge Oktay
    Ticks harbor the largest diversity of microorganisms, ranging from viruses, prokaryotes, and eukaryotes. Rhipicephalus sanguineus ticks are the most common ticks worldwide. Although dogs are the main host of this tick species, it has been reported that it also infests humans in various parts of the world. This study aimed to examine some bacteria (Coxiella burnetii, Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Wolbachia) in Rhipicephalus sanguineus ticks sampled from dogs. In this study, 350 tick samples collected from 85 dogs in Siirt province were determined to be Rhipicephalus sanguineus ticks. Coxiella DNA was detected in 3 (0.85%) out of 350 ticks using Nested PCR (687 base pairs). None of the samples were found to contain Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, and Wolbachia DNA. A partial sequence of the IS1111 gene region was registered in GenBank with OM472143 accession numbers. Considering the zoonotic nature of the Q disease, it is very important for dog owners and related institutions to periodically spray animals against ticks, and to take any other necessary precautions. More samples are needed to determine the Mhc, CMhp, and Wolbachia prevalence. © 2022, Assiut University, Faculty of Veterinary Medicine. All rights reserved.
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    Prevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of Blastocystis spp. in Anatolian Water Buffalo (Bubalus bubalis) in Van, Turkiye
    (Wiley, 2024) Ayan, Adnan; Celik, Burcak Aslan; Celik, Ozgur Yasar; Yilmaz, Ali Bilgin; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
    Background: Blastocystis spp. is one of the most common protozoa worldwide, living in the gastrointestinal tract of humans and many other animals. On the basis of the genetic heterogeneity of small subunit ribosomal RNA, at least 28 subtypes (ST1-ST17, ST21 and ST23-ST32) are reported to exist in mammals and birds. ObjectivesThis study was carried out to determine the prevalence and subtypes of Blastocystis spp. in Anatolian buffaloes (Bubalus bubalis) in Van province in the Eastern Anatolia Region of Turkey. Methods: DNA was extracted using commercial GeneMATRIX Stool DNA Purification Kit and then stored at -20 degrees C until PCR amplification. After PCR amplification of the SSU rRNA gene region positive Blastocystis spp., amplicons from buffalo faeces were sequenced and then deposited in GenBank (OR576949.1, OR576950.1, OR576970.1, OR576971.1, OR577019.1, PP837943.1, PP837940.1, PP837939.1, PP837604.1, PP837937.1, PP837934.1, PP837601.1, PP837936.1 and PP837603.1). Results: PCR analysis of 120 faecal samples showed a total prevalence of 11.67% (14/120). The prevalence was higher in females and young animals (p > 0.05). Sequence analysis revealed Blastocystis spp., ST10, ST14, ST25 and ST26 subtypes. To our knowledge, Blastocystis subtypes ST25 and ST26 in buffaloes were reported for the first time in this study. Conclusion: It is thought that more large-scale studies should be carried out to determine the zoonotic subtype potential of this protozoan in the region.