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Öğe Biochar in conjunction with reduced doses of mineral fertilizers increased yield attributes and yield of rice (cv. BRRI dhan29)(Pakistan Journal of Botany, 2024-08-30) Md. Rayhanul Hoque; S.M Rahman; S.A. Ruba; J. Ferdous; Kamrunahar K.V.; Md. Rafiqul Islam; Zeki Erden; Muhammad Aamir Iqbal; Walid Soufan; Samer Abo Dawaba; Mohammad Sohidul IslamRecently, biochar (BC) applied in optimized quantities has emerged as an effective organic amendment for improving the physico-chemical features of the soil along with boosting the yield attributes of cereals. In the research field of Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur a field experiment was directed to assess the impact of BC implication united with reduced doses of recommended chemical fertilizers (RDF including N, P, K and S) on the growth and yield of rice (cv. BRRI dhan29). It was comprised of five treatments viz. T1= Recommended doses of RDF, T2 = BC 10 t ha-1, T3 = BC 10 t ha-1 + half RDF, T4= Biochar 7.5 t ha-1 + half RDF and T5 = BC 5 t ha-1 + half RDF. The experimental design was the regular arrangement of Randomized Complete Block Design (RCBD) along with four replications. The T1 treatment recorded the maximum plant height (103.00 cm) and the number of tillers hill-1 (26.10) which remained statistically identical to T3. Likewise, T3 out performed rest of regimes by recording the highest values of panicle length (24.89 cm), grains number panicle-1 (195.40), filled grains number panicles-1 (191.10), and 1000-grain weight (25.53 g). Moreover, the same treatment recorded for grain yield (7.82 tha-1) and straw yield (8.76 t ha-1) which was statistically at par to T1 (7.46 and 8.72 t ha-1, respectively). Furthermore, T3 also remained superior as for as biological yield (16.58 t ha-1) of rice was concerned. The outcomes of this trial reveal that BC (10 t ha-1) application have potential to reduce CF dose up to 50% for improving the yield attributes and grain output of rice. (cv. BRRI dhan29).Öğe Mitigation of salinity stress in sunflower plants (Helianthus annuus L.) through topical application of salicylic acid and silver nanoparticles(Springer Science and Business Media LLC, 2024) Muhammad Shahbaz; Tauseef Anwar; Sammer Fatima; Nilgün Onursal; Huma Qureshi; Waseem Akhtar Qureshi; Naimat Ullah; Walid Soufan; Wajid ZamanSalinity stress poses a significant threat to sunflower (Helianthus annuus L.) by impairing water and nutrient uptake, disrupting cellular functions, and increasing oxidative damage. This study investigates the impact of Salicylic acid (SA) and silver nanoparticles (AgNPs) on growth, biochemical parameters, and oxidative stress markers in salt-stressed sunflower plants. Experiments were conducted in a controlled greenhouse environment at the Islamia University of Bahawalpur, Pakistan, using sunflower seeds (Orisun 701). AgNPs were synthesized using neem leaf extract and characterized through SEM, FTIR, zeta potential analysis, and XRD. Treatments included foliar application of SA (10 mM) and AgNPs (40 ppm) under 100 mM sodium chloride-induced salt stress. Growth metrics, antioxidant enzyme activities, chlorophyll content, and oxidative stress markers (H2O2 and MDA levels) were measured to evaluate treatment effects. The SA and AgNP treatments improved sunflower growth under salt stress, with AgNPs showing a greater impact. SA increased shoot fresh weight by 16.4%, root fresh weight by 6.9%, and chlorophyll content by 12.7%, while AgNPs enhanced shoot fresh weight by 30.5%, root fresh weight by 11.6%, and total chlorophyll by 80%. AgNPs also significantly reduced H2O2 by 42.7% and MDA by 34.6%, indicating reduced oxidative damage. Cluster analysis further demonstrated the distinct physiological responses elicited by AgNPs compared to SA. SA and AgNPs enhance sunflower resilience to salinity, with AgNPs showing a particularly strong effect on chlorophyll content and oxidative stress markers. These findings highlight the potential of SA and AgNPs as effective treatments for salt stress, suggesting further research across different crops and environments.Öğe Wheat genotypes respond differently under polyethylene glycol (PEG) induced drought stress during germination and early seedling growth stages(Pakistan Journal of Botany, 2024-08-01) Mst Masuma Akhter; M.A. Hasan; M.M. Bahadur; M.R. Islam; Muhammad Aamir Iqbal; Walid Soufan; Khandakar Aurib; Tanjila Akhter; Sipan Soysal; Ayman Elsabagh; Elsabagh Heniesh; Mohammad Sohidul IslamWater scarcity has emerged as one of a critical environmental constriction that negatively affect wheat growth, development and yield in Bangladesh. Identification of drought tolerant genotypes is crucial at drought sensitive initial stages of plant growth especially germination and seedling growth stages. To cater this issue, 30 wheat genotypes were subjected to polyehelene glycol (PEG 6000) induced drought stress levels of 0,-2.0 and-4.0 Mpa (osmotic stresses) and their response was tested at the germination and seedling growth stages. The experimental set up was Completely Ramdomized Design (CRD) with five replications. The results revealed that the rate of germination (RG) among the genotypes varied significantly with the different water potential levels, and the RG decreased with the advancement of stress. The stress tolerance index (STI) values based on RG were > 0.900 in wheat cultivars of Shatabdi, BARI Gom 25, BAW 1118, BAW 1151, BAW 1161, E 2, E 18, E 30, E 34 and E 38under severe water deficit stress, while the values were < 0.800 in wheat genotypes BARI Gom 26, BARI Gom 27, BARI Gom 28, BAW 1130, BAW 1140, BAW 1143, BAW 1168, E 28 and E 42. The co-efficient of germination (COG) was significantly decreased with increasing water potential stresses (-2 &-4 bars), and the genotypes Sourav, BARI Gom 25, BAW 1118, BAW 1135, BAW 1151, BAW 1157, BAW 1161, BAW 1163, BAW 1170, BAW 1171, E 2, E 18, E 23, E 29, E 34 and E 38showed > 0.980 STI values whereas, BARI Gom 26, BARI Gom 27, E 24, E 28, E 42, BAW 1130, BAW 1140 and BAW 1168 produced < 0.970 STI values under higher water potential. Higher STI values (> 0.920) regarding the germination vigour index (GVI) under higher water stress were observed in BARI Gom 25, E 18, E 23, E 34, E 38, BAW 1118, BAW 1161 and BAW 1170, and very lower STI values (< 0.800) were obtained in the Shatabdi, BARI Gom 26, BARI Gom 27, BAW 1130, BAW 1140, BAW 1168, E 3, E 24, E 28 and E 42 genotypes. The genotypes Shatabdi, BARI Gom 25, E 18, E 38, BAW 1118, BARI Gom 27, E 24, E 34, BAW 1143 and BAW 1170 showed higher STI values (> 0.550) indicating tolerant genotypes and the genotypes Sourav, BARI Gom 26, BARI Gom 28, BAW 1130, BAW 1151, BAW 1168, E 2, E 3, E 28 and E 29demonstrated lower STI values (< 0.450) designating susceptible genotypes. As water deficit stress increased, the shoot weight (g) of wheat genotypes decreased. Notably, genotypes Shatabdi, BARI Gom 25, E 18, E 34, E 38, and BAW 1118 exhibited Stress Tolerance Index (STI) values greater than 0.700. In contrast, genotypes BARI Gom 26, E 2, E 23, E 29, BAW 1130, BAW 1140, BAW 1151, BAW 1157, and BAW 1161 displayed STI values lower than 0.550. The genotypes showing higher and lower STI values denoted water stress tolerant and susceptible genotypes, respectively.