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    Öğe
    In silico analysis of drought responsive transposons and transcription factors in Solanum tuberosum L.
    (2019) Tüfekçi, Ebru Derelli; İnal, Behçet
    Potato (Solanum tuberosum L.) is the most important non-grainfood crop and is essential forglobal food security. Drought is one of the major abiotic stress factors resulting in huge yieldloss in the production of crops and similarly, it negativelly affects the tuberization, tuberyield and tuber quality of potato. Transposable elements (TEs) account for a large portion ofthe genome in many eukaryotic species. Several studies have identified the molecularmechanism that cause the activation of TEs under stress. Stresses and environmentalchallenges, in particular, are known to alter the expression or stimulate the transposition ofmobile elements in plants. Transcription of these stress-responsive genes is largelycontrolled by transcription factors (TFs). A number of transcription factors playing anessential role in drought tolerance of plants have been identified in the past few years. Inthis work, a subset of drought responsive TE families and TFs in potato at tuber bulking stagewas defined, based on genome-wide transposon homology and annotation. Our resultsindicated that, the read numbers for TE subfamilies yielded different distributions betweenDNA and RNA transposons. In addition, many TFs such as bHLH, WRKY, NAC, AP2/ERF mayhave important functions in regulation of drought tolerance in potato. Identification of TEsand TFs which are taking part in stress can offer useful information for functional genomicsand designing novel breeding strategies for developing stress tolerant plants.
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    Öğe
    The Effects of Salicylic Acid Applications on miRNA Expression in Wheat Cultivars under Drought Stress
    (2020) Tüfekçi, Ebru Derelli; İnal, Behçet
    Wheat cultivation in Turkey, is highly affected by environmental factors such as drought stress, which reduces yields significantly.Applying chemicals that stimulate plant growth is an easy, low-cost, low-risk and effective approach to increase stress tolerance, andsalicylic acid is prominent in adapting plants to adverse environmental conditions such as drought stress, salinity, and improving plants'tolerance to stress. In the study, Gün 91 and Ankara 98 cultivars were used as drought tolerant and Ankara 98the sensitive plant material,respectively. Two different doses (0.1 mM, 0.5mM) of salicylic acid (SA) were applied to these plants. Drought stress teratment wasperformed one week after SA application. Total RNAs were isolated from leaf samples collected from application and control pots. Theexpression levels of miR156, miR169, miR172, miR319, mir398 and the genes targeted by these miRNAs were determined. Accordingto the results, in Gün 91, the expression levels of miR156 and miR172 was found higher in leaf tissues treated with SA under droughtstress conditions. On the other hand, the level of miR169 was higher in Ankara 98. In Gün 91, miR319 expression level was higher in0.5 mM salicylic acid + drought treatment, whereas in Ankara 98 was higher in 0.1 mM SA + drought treatment.According to the control group, mir398 expression level decreased significantly in both 91 and Ankara 98 plant groups in which SA +drought stress was applied together. As a result of this study, it was emphasized that salicylic acid is an important signal molecule inresponse to stress and it mitigates the effect in stress conditions, and the tolerance mechanism of drought tolerant and sensitive cultivarswith salicylic acid application were different.