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Öğe Application of magnetized fungal solid phase extractor with Fe2O3 nanoparticle for determination and preconcentration of Co(II) and Hg(II) from natural water samples(Elsevier Science Bv, 2018) Ozdemir, Sadin; Mohamedsaid, Siham Abdullah; Kilinc, Ersin; Yildirim, Aydil; Soylak, MustafaPleurolus eryngii loaded Fe2O3 magnetic nanoparticles were developed as novel magnetic solid phase bio-extractors for the preconcentrations and extractions of Co(II) and Hg(II) from real samples. The metal binding surface groups of the prepared magnetic solid phase bio-extractors were investigated by FT-IR spectrometry, SEM and EDX. Several parameters influencing the magnetic extractions and preconcentrations of Co(II) and Hg (II) were examined and optimized, including pH, flow rate, quantity of P. eryngii, quantity of Fe2O3 magnetic nanoparticle, eluent type, concentration and volume, sample volume, and foreign ions. A good reusage (35 times) was obtained with the relative standard deviation (RSD) of < 5.0% for both magnetic solid phase bio-extractors. The biosorption capacities were determined for Co(II) and Hg(II) as 25.4 mg g(-1) and 30.3 mg g(-1), respectively, using P. eryngii loaded Fe2O3 magnetic nanoparticle column. The preconcentration factors for Co(II) and Hg(II) were found as 80 and 100, respectively, when using P. eryngii loaded Fe2O3 magnetic nanoparticle column. The developed procedures were validated through analysis of the certified reference materials and successfully utilized for the determinations of Co(II) and Hg(II) in real samples by inductively coupled plasma optical emission spectrometry (ICP-OES) with satisfactory results.Öğe Comparative solid phase extraction study on the U(VI) preconcentration by using immobilized thermotolerant Bacillus vallismortis and Bacillus mojavensis(Springer, 2018) Ozdemir, Sadin; Oduncu, M. Kadir; Kilinc, Ersin; Soylak, MustafaBacillus vallismortis and Bacillus mojavensis were loaded onto Amberlite XAD-4 resin and used for solid phase extraction (SPE) of uranium(VI). A quick and simple UV-Vis spectrophotometric method was used to determine U(VI) ion. The best experimental conditions were determined as being a pH of 5.0; a sample flow rate of 2.0 mL min(-1); 200.0 mg of biosorbent; 800 mg of Amberlite XAD-4, and 5.0 mL of 1 mol L-1 HCl as desorption solution for both immobilized bacteria. The preconcentration factors were achieved as 80 for both solid phase extractor. The developed methods were validated by applying to reference water and tea samples.Öğe Magnetic solid phase extractions of Co(II) and Hg(II) by using magnetized C-micaceus from water and food samples(Elsevier Sci Ltd, 2019) Ozdemir, Sadin; Mohamedsaid, Siham Abdullah; Kilinc, Ersin; Soylak, MustafaA new bio-MSPE sorbent based on the use of C. micaceus and gamma-Fe2O3 magnetic nanoparticle was prepared for the preconcentrations of Co(II) and Hg(II). Critical parameters including pH, flow rate, quantity of C. micaceus, quantity of gamma-Fe2O3 magnetic nanoparticle, eluent (type, concentration and volume), sample volume, and foreign ions were examined. Surface structure and variations after interaction with Co(II) and Hg(II) of bio-MSPE sorbent were investigated by FT-IR, SEM, and EDX. The impact of bio-MSPE column reusage was also tested. The biosorption capacities were determined as 24.7 mg g(-1) and 26.2 mg g(-1), respectively for Co(II) and Hg(II). Certified reference materials were utilized to find out the accuracy of the prepared bio-MSPE method. This novel bio-MSPE method was accomplished by being applied to real food and water samples. In particular, it will be possible to make use of C. micaceus as new alternatives, in environmental biotechnology applications.Öğe Phallus impudicus loaded with ?-Fe2O3 as solid phase bioextractor for the preconcentrations of Zn(II) and Cr(III) from water and food samples(Elsevier Sci Ltd, 2020) Yalcin, M. Serkan; Kilinc, Ersin; Ozdemir, Sadin; Yuksel, Uyan; Soylak, MustafaWe investigated the application of fungus Phallus impudicus loaded gamma-Fe2O3 nanoparticles as a biosorbent for magnetic solid phase extractions of trace levels of Zn(II) and Cr(III) ions from natural samples before their measurements by inductively coupled plasma optical emission spectrometry. The characterization of magnetized P. impudicus was performed using the scanning electron microscope, the energy dispersive X-ray and Fourier transform infrared spectroscopy. Important experimental factors were investigated. The experimental results fitted well to the Langmuir adsorption model and pseudo-second order kinetic model. Limit of detections of targeted ions by magnetic solid phase extraction method based on use of P. impudicus were found as 10.5 ngL(-1) and 12.6 ngL(-1) respectively for Cr(III) and Zn(II). The sorption capacities of the biosorbent were 22.8 mgg(-1) for Cr(III) and 25.6 mgg(-1) for Zn(II). The preconcentration factors were achieved as 100 for both of ions. RSDs for inter- and intraday precisions were found as lower than 2.0% and 2.1% respectively for both of targeted ions. The accuracy of the recommended process was tested by recovery measurements on the certificated reference materials and successfully applied for quantification recoveries of Cr(III) and Zn(II) ions from water and food samples.Öğe Preconcentrations of Zn(II) and Hg(II) in Environmental and Food Samples by SPE on B. licheniformis Loaded Amberlite XAD-4(Springernature, 2022) Ozdemir, Sadin; Kilinc, Ersin; Acer, Omer; Soylak, MustafaIn this work, the separations and preconcentrations of Zn(II) and Hg(II) ions on Bacillus lichenifoemis loaded onto Amberlite XAD-4 resin by solid-phase extraction has been performed. The biosorbent was characterized by using FT-IR, SEM, and EDX. pH, sample flow rate, eluent type and concentration, amount of B. licheniformis and XAD-4 resin, sample volume, and possible interfering ions effect were investigated in details as experimental variables in the SPE procedure. Limit of detection values for Zn(II) and Hg(II) were detected as 0.03 and 0.06 ng-mL(-1), respectively. 0.2-15 ng-mL(-1) linear range values were achieved for Zn(II) and Hg(II), respectively. Relative standard deviation values were found to be lower than 5%. For validation of the procedure, the certified standard reference materials (CWW-TM-D, EU-L-2, NCS ZC73O14, NCS ZC73350) were analyzed. The concentrations of Zn(II) and Hg( II) in water and food samples were measured by ICP-OES. Consequently, it can be inferred that the immobilized B. licheniformis microcolumn has ideal selectivity for Zn(II) and Hg(II) biosorption.Öğe Resistance, bioaccumulation and solid phase extraction of uranium (VI) by Bacillus vallismortis and its UV-vis spectrophotometric determination(Elsevier Sci Ltd, 2017) Ozdemir, Sadin; Oduncu, M. Kadir; Kilinc, Ersin; Soylak, MustafaBioaccumulation, resistance and preconcentration of uranium(VI) by thermotolerant Bacillus vallismortis were investigated in details. The minimum inhibition concentration of (MIC) value of U(VI) was found as 85 mg/L and 15 mg/L in liquid and solid medium, respectively. Furthermore, the effect of various U(VI) concentrations on the growth of bacteria and bioaccumulation on B. vallismortis was examined in the liquid culture media. The growth was not significantly affected in the presence of 1.0, 2.5 and 5.0 mg/L U(VI) up to 72 h. The highest bioaccumulation value at 1 mg/L U(VI) concentration was detected at the 72nd hour (10 mg/g metal/dry bacteria), while the maximum bioaccumulation value at 5 mg/L U(VI) concentration was determined at the 48th hour (50 mg metal/dry bacteria). In addition to these, various concentration of U(VI) on alpha-amylase production was studied. The alpha-amylase activities at 0, 1, 2.5 and 5 mg/L U(VI) were found as 3313.2, 3845.2, 3687.1 and 3060.8 U/mg, respectively at 48th. Besides, uranium (VI) ions were preconcentrated with immobilized B. vallismortis onto multiwalled carbon nanotube (MWCNT) and were determined by UV-vis spectrophotometry. The surface macro structure and functionalities of B. vallismortis immobilized onto multiwalled carbon nanotube with and without U(VI) were examined by FT-IR and SEM. The optimum pH and flow rate for the biosorption of U(VI) were 4.0-5.0 and 1.0 mL/min, respectively. The quantitative elution occurred with 5.0 mL of 1 mol/L HCI. The loading capacity of immobilized B. vallismortis was determined as 23.6 mg/g. The certified reference sample was employed for the validation of developed solid phase extraction method. The new validated method was applied to the determination of U(VI) in water samples from Van Lake-Turkey. (C) 2017 Elsevier Ltd. All rights reserved.Öğe Simultaneous preconcentrations of co2+, cr6+, hg2+ and pb2+ ions by bacillus altitudinis immobilized nanodiamond prior to their determinations in food samples by ıcp-oes.(Food Chemistry, 2017) Özdemir, Sadin; Kılınç, Ersin; Okumuş, Veysi; Çelik, Kadir; Soylak, MustafaCo2+, Cr6+, Hg2+ and Pb2+ ions prior to their determinations in food samples by ICP-OES. Thermophilic Bacillus altitudinis immobilized nanodiamond was used as a new biosorbent. SEM and FT-IR analysis were studied to characterize the biosorbent. The optimum pH values of quantitative biosorption for Co2+, Cr6+, Hg2+ and Pb2+ were found to be 5.0, 6.0, 6.0 and 6.0, respectively. A flow rate of 3.0 mL min_1 was selected as optimum for all metal ions. 5 mL of 1 mol/L HCl was used as eluent. Preconcentration factor was achieved as 80. LODs were calculated as 0.071, 0.023, 0.016 and 0.034 ng mL_1, respectively for Hg2+, Co2+, Cr6+ and Pb2+. The biosorption capacities were calculated for Co2+, Cr6+, Hg2+ and Pb2+ as 26.4, 30.4, 19.5, and 35.2 mg/g, respectively. The developed method was successfully applied to food samples to determine analyte concentrations.Öğe Simultaneous preconcentrations of Cu(II), Ni(II), and Pb(II) by SPE using E. profundum loaded onto Amberlite XAD-4(Elsevier, 2021) Ozdemir, Sadin; Kilinc, Ersin; Acer, Omer; Soylak, MustafaIn the present study, we used E. profundum loaded onto Amberlite XAD-4 resin for solid-phase extraction (SPE) of Cu(II), Ni(II), and Pb(II). Some conditions of the SPE procedure, for instance, sample flow rate, pH, eluent type and concentration, the sample volume, amount of E. profundum XAD-4 resin, and the effects of foreign in-terferences ions are discussed in detail. Surface microstructures of the biosorbent were characterized by FTIR, FE-SEM/EDX before and after loading of metals. The biosorption capacities of E. profundum were determined as 49.83, 57.12, and 64.35 mg g(-1) for Pb(II), Cu(II), and Ni(II), respectively. The LOD values were calculated as 0.031 ng mL(-1), 0.042 ng mL(-1), and 0.043 ng mL(-1) for Cu(II), Ni(II), and Pb(II), respectively. The linear range was detected as 0.25-25 ng mL(-1). RSD values were lower than 5%. The concentrations of metal ions in water and food samples were measured by ICP-OES.Öğe Tolerance and bioaccumulation of U(VI) by Bacillus mojavensis and its solid phase preconcentration by Bacillus mojavensis immobilized multiwalled carbon nanotube(Academic Press Ltd- Elsevier Science Ltd, 2017) Ozdemir, Sadin; Oduncu, M. Kadir; Kilinc, Ersin; Soylak, MustafaIn this study, uranium(VI) tolerance and bioaccumulation were investigated by using thermo-tolerant Bacillus mojavensis. The level of U(VI) was measured by UV-VIS spectrophotometry. The minimum inhibition concentration (MIC) value of U(VI) was experimented. Bacterial growth was not affected in the presence of 1.0 and 2.5 mg/L U(VI) at 36 h and the growth was partially affected in the presence of 5 mg/L U(VI) at 24 h. What was obtained from this study is that there was diversity in the various periods of the growth phases of metal bioaccumulation capacity, which was shown by B. mojavensis. The maximum bioaccumulation capacities were found to be 12.8, 22.7, and 48.2 mg/g dried bacteria, at 24th hours at concentration of 1.0, 2.5 and 5 mg/L U(VI), respectively. In addition to these, U(VI) has been pre-concentrated on B. mojavensis immobilized MWCNT. Several factors such as pH, flow rate of solution, amount of biosorbent and support materials, eluent type, concentration and volume, the matrix interference effect on retention have been studied, and extraction conditions were optimized. Preconcentration factor was achieved as 60. Under the optimized conditions, the limit of detection (LOD) and quantification (LOQ) were calculated as 0.74 and 2.47 mu g/L. The biosorption capacity of immobilized B. mojavensis was calculated for U(VI) as 25.8 mg/g. The results demonstrated that the immobilized biosorbent column could be reused at least 30 cycles of biosorption and desorption with the higher than 95% recovery. FT-IR and SEM analysis were performed to understand the surface properties of B. mojavensis. (C) 2016 Elsevier Ltd. All rights reserved.
