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Öğe Diallel Analysis and Selection of Hybrids for Nutritional Phytochemicals in Capsicum Annuum L.(Hard, 2024) Chakrabarty, Swapan; Ahamed, Tofayel; Ditta, Allah; Pandey, Saurabh; Cig, Arzu; Soufan, Walid; El Sabagh, AymanChili (Capsicum annuum L.) is an important functional food due to its main bioactive compound, capsaicin, and other nutritional phytochemicals. However, very few studies have been conducted to develop hybrids with a high content of nutritional phytochemicals. The present study involving six parents was conducted to identify superior hybrids with higher nutritional quality based on combining ability and heterosis following Griffing's diallel Method II Model I. A broad spectrum of genetic variation among the six parents and fifteen F1 hybrids was confirmed by analysis of variance. (H1/D)0.5 value indicated that partial dominance gene action controlled all the traits except capsaicin and total phenolic content. Based on general combining ability (GCA) results, parent P3 (PLP-2s) was the best general combiner for all the traits except K and Na, followed by the parents P6 (BU Capsicum 1), P5 (Morich-8), P4 (Chili Japan) and P1 (Red Chili). Specific combining ability (SCA), along with heterotic response, revealed that the F1 hybrid P3xP6 (PLP-2s x BU Capsicum 1) was the best hybrid, followed by the hybrids P4xP6 (Chili Japan x BU Capsicum 1) and P3xP4 (PLP-2s x Chili Japan), as they exhibited superiority for major nutritional components, such as capsaicin and ascorbic acid. Ultimately, the subsequent selection of the F1 hybridsÖğe Effect of NaCl on physiological, biochemical, and ionic parameters of naked oat (Avena nuda L.) line Bayou1(Frontiers Media Sa, 2024) Liu, Liyun; Assaha, Dekoum V. M.; Islam, Mohammad Sohidul; Rajendran, Karthika; Theivasigamani, Parthasarathi; Soufan, Walid; Ayman, El SabaghOat (Avena nuda L.) is a globally important cereal crop grown for its nutritious grains and is considered as moderately salt-tolerant. Studying salinity tolerant mechanisms of oats could assist breeders in increasing oat production and their economic income in salt-affected areas, as the total amount of saline land in the world is still increasing. The present study was carried out to better understand the salt tolerance mechanism of the naked oat line Bayou1. A soil experiment was conducted on 17 days-old Bayou1 seedlings treated with varying concentrations of NaCl for a period of 12 days. Bayou1 plants grew optimally when treated with 50 mM NaCl, demonstrating their salinity tolerance. Reduced water uptake, decreased Ca2+, Mg2+, K+, and guaiacol peroxidase activity, as well as increased Na+ concentration in leaves, all contributed to a reduction in shoot growth. However, the damage to ionic homeostasis caused by increased Na+ concentrations and decreased K+ concentrations in the roots of Bayou1 did not inhibit its root growth, indicating that the main salt-tolerant mechanism in Bayou1 existed in its roots. Further, a hydroponic experiment found that increasing Na+ concentration in root cell sap enhanced root growth, while maintaining the integrity of root cell membranes. The accumulated Na+ may have facilitated the root growth of Bayou1 exposed to NaCl by effectively adjusting cellular osmotic potential, thereby ensuring root cell turgor and expansion.Öğe Exploring the therapeutic potential of the oxygenated monoterpene linalool in alleviating saline stress effects on Allium cepa L.(Springer, 2024) de Lima Silva, Jailson Renato; dos Santos, Larisse Bernardino; Hassan, Waseem; Kamdem, Jean Paul; Duarte, Antonia Eliene; Soufan, Walid; El Sabagh, AymanSodium chloride (NaCl) can cause oxidative stress in plants, which represents a potential obstacle to the development of monocultures worldwide. Onion (Allium cepa L.) is a famous vegetable consumed and used in world cuisine. In the present study, we analyzed the influence of soil physicochemical profile and the remedial capacity of linalool on seed emergence, roots, and leaf growth in onions subjected to salt stress, as well as its in vivo and in vitro antioxidant potential, Fe2+chelating activity, and reducing power of Fe3+. The outcome of the soil analysis established the following order of abundance: sulfur (S) > calcium (Ca) > potassium (K) > magnesium (Mg) > sodium (Na). NaCl (150 mM) significantly reduced the emergence speed index (ESI), leaf and root length, while increasing the peroxidation content. The length of leaves and roots significantly increased after treatment with linalool (300 and 500 ?g/mL). Our data showed negative correlations between seed emergence and K+ concentration, which was reversed after treatments. Linalool (500 ?g/mL) significantly reduced oxidative stress, but increased Fe2+ concentration and did not show potential to reduce Fe3+. The in vivo antioxidant effect of linalool is thought to primarily result from an enzymatic activation process. This mechanism underscores its potential as a therapeutic agent for oxidative stress–related conditions. Further investigation into this process could unveil new avenues for antioxidant therapy. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.Öğe Modern Breeding Approaches for Climate Change(Springer International Publishing, 2023) Afzal, Sadia; Mubeen, Muhammad; Hussain, Sajjad; Ali, Mazhar; Rashad Javeed, Hafiz Muhammad; Al-Ashkar, Ibrahim; Soufan, WalidClimate-smart agriculture is the emerging and sustainable option to mitigate the adverse effects of climate change (on crop adaptability) before it significantly influences global crop production. Crop development through modern breeding techniques, effective agronomic practices and exploitation of natural variability in neglected and popular crops are all good ways to meet future food demands. However, the rapidly changing environment requires technological interventions to improve crop climate resilience. Technological advances such as genome-edited transgenic plants, high-throughput phenotyping technologies combined with next-generation sequencing techniques, big data analytics and advances in modern breeding techniques help modern agriculture progress towards robotics or digital conversion to face future environmental adversaries. For example, speed breeding in combination with genomic and phenomic methods can lead to quicker identification of genetic factors and, as a result, speed up crop development programmes. Furthermore, combining next-generation interdisciplinary breeding platforms might open up new opportunities for developing climate-ready crops. Several integrated modern breeding platforms were created in the last few decades and are now employed worldwide. Africa and Asia have adopted these most frequently used crop improvement platforms with advanced techniques like multitrait association studies using genome-wide association studies (GWASs). These have permitted precise exploration of the genetic make-up of agricultural attributes in most crops. This chapter explores various ways to increase crop output by developing climate-resilient superior genotypes. Further, we discussed how combinatorial advanced breeding technologies and biotechnological approaches would be used for managing climate change’s consequences to promote crops with climate resilience. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.Öğe Synergistic effects of melatonin and glycine betaine on seed germination, seedling growth, and biochemical attributes of maize under salinity stress(Wiley, 2024) Su, Wennan; Qiu, Jiaoqi; Soufan, Walid; El Sabagh, AymanSalinity stress represents a major threat to crop production by inhibiting seed germination, growth of seedlings, and final yield and, therefore, to the social and economic prosperity of developing countries. Recently, plant growth-promoting substances have been widely used as a chemical strategy for improving plant resilience towards abiotic stresses. This study aimed to determine whether melatonin (MT) and glycine betaine (GB) alone or in combination could alleviate the salinity-induced impacts on seed germination and growth of maize seedlings. Increasing NaCl concentration from 100 to 200 mM declined seed germination rate (4.6-37.7%), germination potential (24.5-46.7%), radical length (7.7-40.0%), plumule length (2.2-35.6%), seedling fresh (1.7-41.3%) and dry weight (23.0-56.1%) compared to control (CN) plants. However, MT and GB treatments lessened the adverse effects of 100 and 150 mM NaCl and enhanced germination comparable to control plants. In addition, results from the pot experiments show that 200 mM NaCl stress disrupted the osmotic balance and persuaded oxidative stress, presented by higher electrolyte leakage, hydrogen peroxide, superoxide radicals, and malondialdehyde compared to control plants. However, compared to the NaCl treatment, NaCl+MT+GB treatment decreased the accumulation of malondialdehyde (24.2-42.1%), hydrogen peroxide (36.2-44.0%), and superoxide radicals (20.1-50.9%) by up-regulating the activity of superoxide dismutase (28.4-51.2%), catalase (82.2-111.5%), ascorbate peroxidase (40.3-59.2%), and peroxidase (62.2-117.9%), and by enhancing osmolytes accumulation, thereby reducing NaCl-induced oxidative damages. Based on these findings, the application of MT+GB is an efficient chemical strategy for improving seed germination and growth of seedlings by improving the physiological and biochemical attributes of maize under 200 mM NaCl stress.Öğe ZINC BIOFORTIFICATION OF MUNGBEAN ( VIGNA RADIATA L.) CULTIVARS THROUGH ZINC CHEMO-PRIMING(Pakistan Botanical Soc, 2024) Awal, Abdul; Hossain, Alamgir; Iqbal, Muhammad aamir; Soufan, Walid; Erman, Murat; Ammar, Hajer; Elsabagh, AymanAmong micronutrients, zinc (Zn) is one of the most vital elements required for the growth and development of human and plants. The deficiency of Zn in Bangladesh's soil is causing a huge population of the country malnourished especially due to low Zn content of pulses like mungbean. A two year's research was conducted during pre -monsoon season of 2022 and 2023 in the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to evaluate the seed priming for Zn biofortification of recently released eight varieties of mungbean (BARI Mug -5, BARI Mug -6, BARI Mug -7 and BARI Mug -8 were grown during 2022 year, the other four varieties named BARI Mug -2, BARI Mug -3, BARI Mug -4 and BINA Mug -8 were grown during the growth season of 2023). Mungbean seeds were soaked for 6 hours before sowing in water (hydropriming), or aerated Zn solution (as osmopriming) of 0.01M or 0.05M with 1:5 seed to liquid ratio. The results showed that seed priming with 0.01M Zn remained unmatched by improving the yield attributes including plant height and number of primary branches per plant and grain yield of mungbean. In addition, seed priming with water and 0.01M Zn solution increased the grain yield by 7 and 15% and grain Zn concentrations by 9 and 14%, respectively compared to control. Overall, BARI Mug -3, BARI Mug -5 and BARI Mug -6 surpassed rest of varieties in terms of grains Zn content. Although Zn enrichment in mungbean grain was observed from the crops grown with primed seed through 0.05M Zn solution, but the performance of plant growth, yield traits and grain yield were noticed significantly poor. Based on recorded findings, it may be recommended that mungbean seeds might be primed with low concentrated zinc solution like 0.01M to attain better stand development and yield attributes, grain yield and grain Zn content.