Yazar "Sen, Fatih" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • [ X ]
    Öğe
    Antioxidant, antimicrobial, cytotoxic and protective effects of truffles
    (Academic Press Inc Elsevier Science, 2022) Fidan, Mehmet; Ali, Muhammad Muddassir; Erez, Mehmet Emre; Cigerci, Ibrahim Hakki; Ozdemir, Sadin; Sen, Fatih
    Fungi can be used as a potent chemotherapeutic agent to treat various cancers. In current study acetone and methanol extracts of Terfezia claveryi, Terfezia boudieri, Terfezia olbiensis, Picoa lefebvrei, Picoa juniperi were used to assess total phenolic contents, antioxidant activity, ion-chelating impact, antimicrobial activity, the cytotoxic and protective effects. Both methanol and acetone extracts of T. boudieri had the highest FRAP and DPPH scavenging abilities. Dose-dependent increased ion-chelating impact of all tested truffles species was found. Extracts of T. boudieri, T. claveryi, and T. albiensis exhibited higher antimicrobial activities. T. claveryi and T. boudieri showed the highest protective effects against H2O2-induced genotoxicity (P < 0.05), in S. cerevisiae BY4741. The least protective effect was showed by the acetone extracts of T. olbiensis (144 +/- 8); methanol ex-tracts of P. lefebvrei (140 +/- 8) and P. juniperi (140 +/- 10). MCF 7 cells showed more sensitivity against to methanol extracts of T. boudieri at 10-100 mu g/mL concentrations. HepG2 cells showed more sensitivity against the methanolic extracts of T. boudieri at both doses. Overall, P. lefebvrei and P. juniperi extracts had the least cytotoxic effects. The species of Terfezia exhibit significant protective effects against DNA damage and also have the potential of cytotoxicity effects.
  • [ X ]
    Öğe
    Impact of Postharvest Putrescine Treatments on Phenolic Compounds, Antioxidant Capacity, Organic Acid Contents and Some Quality Characteristics of Fresh Fig Fruits during Cold Storage
    (Mdpi, 2023) Kucuker, Emine; Aglar, Erdal; Sakaldas, Mustafa; Sen, Fatih; Gundogdu, Muttalip
    The storage and shelf life of the fig, which has a sensitive fruit structure, is short, and this results in excessive economic losses. In a study carried out to contribute to the solution of this problem, the effect of postharvest putrescine application at different doses (0, 0.5, 1.0, 2.0, and 4.0 mM) on fruit quality characteristics and biochemical content during cold storage in figs was determined. At the end of the cold storage, the decay rate and weight loss in the fruit were in the ranges of 1.0-1.6% and 1.0-5.0 %, respectively. The decay rate and weight loss were lower in putrescine-applied fruit during cold storage. Putrescine application had a positive effect on the changes in fruit flesh firmness values. The SSC rate of fruit varied between 14 and 20%, while significant differences in the SSC rate occurred depending on storage time and putrescine application dose. With putrescine application, the decrease in the acidity rate of the fig fruit during cold storage was smaller. At the end of the cold storage, the acidity rate was between 1.5-2.5% and 1.0-5.0. Putrescine treatments affected total antioxidant activity values and changes occurred in total antioxidant activity depending on the application dose. In the study, it was observed that the amount of phenolic acid in fig fruit decreased during storage and putrescine doses prevented this decrease. Putrescine treatment affected the changes in the quantity of organic acids during cold storage, and this effect varied depending on the type of organic acid and the length of the cold storage period. As a result, it was revealed that putrescine treatments can be used as an effective method to maintain postharvest fruit quality in figs.
  • [ X ]
    Öğe
    Investigation of Some Physicochemical Traits and Changes After Storage of Fruits Belonging to 'Zivzik' Pomegranate Genotypes Grown in Siirt, Turkey
    (Springer, 2022) Ozrenk, Koray; Turk, Bilge; Sen, Fatih; Keskin, Nurhan; Kaya, Ozkan
    Pomegranate genotypes called 'Zivzik' are widely grown in Siirt, Turkey, and these genotypes have sweet, tart, and sour fruits. This study was carried out to determine the physicochemical traits of fruits belonging to 'Zivzik' pomegranate genotypes, as well as changes in fruit characteristics after storage. Sweet, tart, and sour 'Zivzik' pomegranate genotypes were harvested in Sirvan and Pervari districts of Siirt. The fruits were placed in modified atmosphere packages and kept at 6 degrees C and 90% relative humidity for 3 months. Some physicochemical traits were determined in both harvested and stored pomegranate fruits. Fruit weight (280.8 g), berry weight (0.41 g), fruit width (84.5 mm), and fruit length (8.57 mm) of the fruits of the sweet pomegranate genotype were found to be higher than in the other genotype fruits. However, the total soluble solids content (17.57%), titratable acid content (3.02 g/100 ml), total phenol content (163.43 mg GAE/100 ml), and antioxidant activity (49.95 mu mol TE/ml) of the fruits of the sour pomegranate genotype were higher than in fruits of other genotypes. There were differences between pomegranate genotypes in fruit peel color and berry size. After storage, there was a significant decrease in the titratable acid content of fruits in all genotypes, while their total soluble solids, total phenol content, and antioxidant activities increased. However, no significant change was found in fruit peel color in any genotype. Consequently, there were differences between the physicochemical traits of the fruits belonging to the sweet, tart, and sour 'Zivzik' pomegranate genotypes, but there were no significant changes in these traits after storage.
  • [ X ]
    Öğe
    Purification and characterization of thermostable a-amylase produced from Bacillus licheniformis So-B3 and its potential in hydrolyzing raw starch
    (Pergamon-Elsevier Science Ltd, 2021) Fincan, Sema Aguloglu; Ozdemir, Sadin; Karakaya, Adem; Enez, Baris; Mustafov, Sibel Demiroglu; Ulutas, Mehmet Sefa; Sen, Fatih
    Aims: This work was achieved to obtain the optimum culture conditions of the thermostable alpha-amylase produced by thermophilic Bacillus licheniformis SO-B3. Furthermore, the alpha-amylase was purified and then characterized, and also its kinetic parameters were determined. Materials and methods: A new thermotolerant bacteria called Bacillus licheniformis SO-B3 employed in this work was isolated from a sample of thermal spring mud in Sirnak (Meyremderesi). Several parameters such as the impact of temperature, time, and pH on enzyme production were examined. Thin-Layer Chromatography (TLC) was employed to analyze the end-products of soluble starch hydrolysis, and the utilization of purified alpha-amylase in the clarification of unripe apple juices was studied. Key findings: The highest enzyme production conditions were determined as 35 degrees C, 36th hour, and pH 7.0. Thermostable alpha-amylase was purified by 70% ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and dialysis, with a 51-purification fold and 30% yield recovery. The K-m and V-max values for this enzyme were 0.004 mM and 3.07 mu mol min(-1) at 70 degrees C, respectively. The alpha-amylase's molecular weight was found as 74 kDa. In addition, alpha-amylase showed a good degradation rate for raw starch. Significance: It was hypothesized that Bacillus licheniformis SO-B3 could be used as an alpha-amylase source. These findings displayed that purified enzyme could be utilized in fruit juice industries for clarification of apple juice and raw starch hydrolyzing.
  • [ X ]
    Öğe
    Resistance, removal, and bioaccumulation of Ni (II) and Co (II) and their impacts on antioxidant enzymes of Anoxybacillus mongoliensis
    (Elsevier Science Inc, 2020) Akkoyun, Mahire Bayramoglu; Ozdemir, Sadin; Kilinc, Ersin; Birhanli, Emre; Aygun, Aysenur; Sen, Fatih
    In this study, it was hypothesis that A. mongoliensis could be used as bioindicator for Ni (II) and Co (II). Thus, Ni (II) and Co (II) resistance, removal, bioaccumulation, and the impacts of them on antioxidant enzyme systems of thermophilic Anoxybacillus mongoliensis were investigated in details. The bioaccumulation of Ni (II) and Co (II) on the cell membrane of thermophilic A. mongoliensis, variations on surface macrostructure and functionality by FT-IR and SEM, and determination of antioxidant enzyme activities were also tested. The highest bioaccumulation values of Co (II) and Ni (II) were detected as 102.0 mg metal/g of dry bacteria at 10 mg/L for the 12th h and 90.4 mg metal/g of dry bacteria for the 24th h, respectively, and the highest Ni (II) and Co (II) cell membrane bioaccumulation capacities of A. mongoliensis were determined as 268.5 and 274.9 mg metal/g wet membrane, respectively at the 24th h. In addition, increasing on SOD and CAT activities were observed on depend of concentration of Ni (II) and Co (II) with respect to control. The antioxidant enzyme activity results also indicated that A. mongoliensis might be used as a bioindicator for Ni (II) and Co (II) pollution in environmental water specimens.