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    Myelosuppression and Oxidative Stress Induced by Cyclophosphamide in Rats: The Protective Role of Selenium
    (2019) Ayhancı, Adnan; Heybeli, Nilhan; Sahin, İlknur Kulcanay; Cengiz, Mustafa
    Aim of this study is to detect the protective role of selenium (Se) against bone marrow and blood toxicity in CP-induced hematoxicity model. We categorized the rats into 6 groups of 7 animals in each group (control; 150 mg/kg CP; 0.5 mg/kg Se; 1 mg/kg Se; 150+0.5 mg/kg CP+Se; 150+1 mg/kg CP+Se). Se injections started 5 days before the CP injections and carried on until the end of the experiment (6th day) for the groups to which CP was injected together with Se. CP was applied as a single dose before anesthesia. For that reason, on the 7th day, blood was taken with cardiac puncture and bone marrow was taken by flushing the femur. Peripheral blood cells and bone marrow nucleated cells were counted on a cell counter. Intraperitoneal CP injection was found to reduce the number of leukocytes by 317%, thrombocyte by 36% and bone marrow nucleated cells by 481% compared to the control group. In the groups where CP was given after 0.5 and 1 mg/kg Se, numbers of leukocyte, thrombocyte and bone marrow nucleated cells were considerably improved compared to the group to which CP was given only (p<0.001). Results show that 1 mg/kg Se has a better protection than 0.5 mg/kg against CP associated hematoxicity and myelosuppression. Our results also imply that the doses of Se could be adjusted according to enhance in CP dose so as to gain a stronger protective effect. We believe there is a need of further studies in which different doses of Se will be used against CP induced hematoxicity. Se can provide protection against CP-induced myelosupression and lipid peroxidation.
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    Öğe
    The protective effects of Selenium and Boron against Cyclophosphamide-induced bone marrow and blood toxicity: An in vivo study
    (2022) Ayhancı, Adnan; Lafcı, Nilüfer; Musmul, Ahmet; Gür, Fatma Özabacıgil; Sezer, Canan; Sahin, İlknur Kulcanay; Gür, Bahri
    Thanks to their antioxidant, anti-apoptotic, anti-lipid peroxidative, and immune-boosting properties, Boron (B) and Selenium (Se) are essential trace elements for the human body. This study aims to compare the myeloid protective potentials of Se and B in Cyclophosphamide (CP)-induced bone-marrow and haematological toxicity in experimental rats considering that the myelotoxic property of this anti-cancer drug limits its use. We hypothesized that selenium has a better protective effect than boron in preventing the toxic effects of CP on bone marrow and blood cells. 1.5 mg/kg of Se and 20 mg/kg of B, which are the most frequently used optimal doses of these trace elements, were given to the animals intraperitoneally throughout the experiment. 200 mg/kg of CP was administered only on the 4th day. The animals were sacrificed to take the blood and bone marrow samples to be stored for hematological evaluations. The CP administration significantly decreased leukocyte (WBC), thrombocyte (PLT), erythrocytes (RBC), and bone marrow nucleated cell counts. On the other hand, they increased in significant amounts in the groups given Se and B along with CP when compared to those given only CP. However, Se proved to be more protective than B in preventing CP-induced bone marrow and hematologic toxicity despite not achieving statistical significance. It was, therefore, concluded that the doses used in this experiment were successful in protecting against CP-induced damage to the bone marrow and CP-related hematological toxicity.