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    Evaluation of various biochemical parameters in the serum and tissues of lambs with white muscle disease
    (Agricultural Research Communication Centre, 2021) Irak, Kivanç; Mert, Handan; Mert, Nihat; Ayşin, Nesrullah; Söğütlü, İnci Doğan; Comba, Arzu
    Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined. Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups. © 2021 Agricultural Research Communication Centre. All rights reserved.
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    Nitrate and Nitrite Levels in Serum and Seminal Plasma in Horses
    (2018) Aslan, Leyla Mis; Mert, Handan; Söğütlü, İnci Doğan; Pala, Nihat Mert; İrak, Kıvanç
    Nowadays, the fertility of the growing horses is of great importance. The aim of our study is to determine and compare the level of nitric oxide oxidation products of these animals in serum and seminal plasma. Seven healthy British horses were used in the study. Blood samples were taken from the vena jugularis. The sera were removed by centrifugation. Seminal plasmas of ejaculates taken with artificial vagina were separated. Seminal plasma and serum were obtained and nitrate nitrite values were determined. Nitrate and nitrite levels of samples were measured by coupling reagent method. Nitrate levels in both semis and seminal plasma were 6.34 ± 2.7 and 4.95 ± 1.4, respectively. Nitrite levels were higher than seminal plasma in seminal plasma (p? 0.05), while nitrite levels in seminal plasma were higher than serum (p? 0.05). As a result, seminal plasma and serum nitrate-nitrite values in British stallions were used. Presented to the knowledge of clinical veterinary medicine.
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    Some Mineral Substance, Oxidative Stress and Total Antioxidant Levels in Norduz and Morkaraman Sheep
    (2018) Aslan, Leyla Mis; Mert, Handan; Comba, Bahat; Söğütlü, İnci Doğan; Irak, Kıvanç; Mert, Nihat
    The aim of the study was to compare some mineral substances and total antioxidant and totaloxidan levels of healthy Morkaraman and Norduz sheep breeds in the same age and care conditions. A total of 20 animals, 10 from each sheep breed, were included in the study. Serum total oxidant status (TOS) and total antioxidant status (TAS) were determined by a colorimetric method. OSI value was determined by calculating the TAS-TOS ratio. When the two sheep were compared, there was a difference between TAS, Mg, P levels. Antioxidant levels of Norduz sheep were higher than morkaraman sheep. The given data may be the reference value for studies of these sheep breeds.