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    Acinetobacter mesopotamicussp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey
    (Springer, 2020) Acer, Omer; Guven, Kemal; Poli, Annarita; Di Donato, Paola; Leone, Luigi; Buono, Lorena; Guven, Reyhan Gul
    A new petroleum-degrading bacterium, designated strain GC2(T), was isolated from Bozkus 1 petroleum station in Diyarbakir, located in the southeast of Turkey. Cells were Gram-negative staining, aerobic, coccoid-rods, non-motile, non-spore-forming. The bacterium was found to degrade 100% ofn-alkanes ranging from C11 to C34 presented in the 1% crude oil after incubation of 7 days. The membrane phospholipids were 1,2 diacylglycero-3-phosphorylethanolamine (PEA), phosphatidylglycerol (PG), dipalmitoyl-sn-glycerol 1- phosphocholine (PC1), 1,2 dipalmitoyl-sn-glycero-3-phosphocholine monohydrate (PC3), cardiolipin also called diphosphatidylglycerol (CL) and l-alpha- phosphatidic acid, dipalmitoyl (AP); predominant respiratory ubiquinone was Q-8 and C16:0, C18:1 omega 9c and C16:1 were the major cellular fatty acids. The 16S rRNA sequence analysis revealed that the strain GC2(T)was a member of genusAcinetobacterand was most closely related toAcinetobacter lwoffiiDSM 2403( T)(99.79%),Acinetobacter pseudolwoffiiANC 5318( T)(98.83%) andAcinetobacter harbinensisHITLi 7( T)(98.14%). TherpoBandgyrBgene sequence analysis confirmed that the strain GC2(T)was a member of genusAcinetobacterand that the closest relative wasAcinetobacter lwoffiiDSM 2403( T)(99.08% and 100% similarity, respectively). DNA-DNA hybridization values between GC2(T)and its closest relatives ranged from 65.6% (withA. lwoffii) to 5.1% (withA. venetianus). The whole genome sequence of strain GC2(T)was obtained. The DNA G + C content of this strain was determined to be 42.9 mol %. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain GC2(T)represents an independent genomospecies. On the basis of phenotypic characteristics, chemotaxonomic, phylogenetic data and DNA-DNA hybridization and whole genome analysis, we propose to assign strain GC2(T)as a new species of the genusAcinetobacter, for which the nameAcinetobacter mesopotamicussp. nov. is proposed. The type strain of this species is GC2(T)(DSM 26953 T = JCM 31073 T). The whole genome of strain GC2(T)has been deposited at DDBJ/ENA/GenBank under the accession JAALFF010000000.
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    Biosorption of Heavy Metals (Cd2+, Cu2+, Co2+, and Mn2+) by Thermophilic Bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus: Equilibrium and Kinetic Studies
    (Taylor & Francis Inc, 2013) Ozdemir, Sadin; Kilinc, Ersin; Poli, Annarita; Nicolaus, Barbara
    Two strains of thermophilic bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus, were employed to investigate the biosorption of heavy metals including Cd2+, Cu2+, Co2+, and Mn2+ ions. The effects of different biosorption parameters such as pH (2.010.0), initial metal concentrations (10.0300.0mg L-1), amount of biomass (0.2510g L-1), temperature (3080 degrees C), and contact time (15120min) were investigated. Concentrations of metal ions were determined by using an inductively coupled plasma optical emission spectrometry (ICP-OES). Optimum pHs for Cd2+, Cu2+, Co2+, and Mn2+ biosorption by Geobacillus thermantarcticus were found to be 4.0, 4.0, 5.0, and 6.0, respectively. For Anoxybacillus amylolyticus, the optimum pHs for Cd2+, Cu2+, Co2+, and Mn2+ biosorption were found to be 5.0, 4.0, 5.0, and 6.0, respectively. The Cd2+, Cu2+, Co2+, and Mn2+ removals at 50mg L-1 in 60min by 50mg dried cells of Geobacillus thermantarcticus were 85.4%, 46.3%, 43.6%, and 65.1%, respectively, whereas 74.1%, 39.8%, 35.1%, and 36.6%, respectively, for Anoxybacillus amylolyticus. The optimum temperatures for heavy metal biosorption were near the optimum growth temperatures for both strains. Scatchard plot analysis was employed to obtain more compact information about the interaction between metal ions and biosorbents. The plot results were further studied to determine if they fit Langmuir and Freundlich models.
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    Öğe
    Resistance and bioaccumulation of Cd2+, Cu2+, Co2+ and Mn2+ by thermophilic bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus
    (Springer, 2013) Ozdemir, Sadin; Kilinc, Ersin; Nicolaus, Barbara; Poli, Annarita
    In this study, bioaccumulation and heavy metal resistance of Cd2+, Cu2+, Co2+ and Mn2+ ions by thermophilic Geobacillus thermantarcticus and Anoxybacillus amylolyticus was investigated. The bacteria, in an order with respect to metal resistance from the most resistant to the most sensitive, was found to be Mn2+ > Co2+ > Cu2+ > Cd2+ for both G. thermantarcticus and A. amylolyticus. It was determined that the highest metal bioaccumulation was performed by A. amylolyticus in Mn2+ (28,566 mu g/g dry weight), and the lowest metal bioaccumulation was performed by A. amylolyticus in Co2+ (327.3 mu g/g dry weight). The highest Cd2+ capacities of dried cell membrane was found to be 36.07 and 39.55 mg/g membrane for G. thermantarticus and A. amylolyticus, respectively, and the highest Cd2+ capacities of wet cell membrane was found to be 14.36 and 12.39 mg/g membrane for G. thermantarcticus and A. amylolyticus, respectively.
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    Thermophilic Geobacillus galactosidasius sp nov loaded ?-Fe2O3 magnetic nanoparticle for the preconcentrations of Pb and Cd
    (Elsevier Sci Ltd, 2016) Ozdemir, Sadin; Kilinc, Ersin; Okumus, Veysi; Poli, Annarita; Nicolaus, Barbara; Romano, Ida
    Thermophilic bacteria, Geobacillus galactosidasius sp nov. was loaded on gamma-Fe2O3 magnetic nanoparticle for the preconcentrations of Pb and Cd by solid phase extraction before ICP-OES. pH and flow rate of the solution, amounts of biosorbent and magnetic nanoparticle, volume of sample solution, effects of the possible interferic ions were investigated in details. Linear calibration curves were constructed in the concentration ranges of 1.0-60 ng mL (1) for Pb and Cd. The RSDs of the method were lower than 2.8% for Pb and 3.8% for Cd. Certified and standard reference samples of fortified water, wastewater, poplar leaves, and simulated fresh water were used to accurate the method. LOD values were found as 0.07 and 0.06 ng mL (1) respectively for Pb and Cd. The biosorption capacities were found as 34.3 mg g (1) for Pb and 37.1 mg g (1) for Cd. Pb and Cd concentrations in foods were determined. Surface microstructure was investigated by SEM-EDX. (C) 2015 Elsevier Ltd. All rights reserved.
  • Küçük Resim
    Öğe
    Thermophilic geobacillus galactosidasius sp. nov. loaded fe2o3 magnetic nanoparticle for the preconcentrations of pb and cd.
    (Bioresource Technology, 2016) Özdemir, Sadin; Kılınç, Ersin; Okumuş, Veysi; Poli, Annarita; Nicolaus, Barbara; Romano, Ida
    Thermophilic bacteria, Geobacillus galactosidasius sp nov. was loaded on c-Fe2O3 magnetic nanoparticle for the preconcentrations of Pb and Cd by solid phase extraction before ICP-OES. pH and flow rate of the solution, amounts of biosorbent and magnetic nanoparticle, volume of sample solution, effects of the possible interferic ions were investigated in details. Linear calibration curves were constructed in the concentration ranges of 1.0–60 ng mL_1 for Pb and Cd. The RSDs of the method were lower than 2.8% for Pb and 3.8% for Cd. Certified and standard reference samples of fortified water, wastewater, poplar leaves, and simulated fresh water were used to accurate the method. LOD values were found as 0.07 and 0.06 ng mL_1 respectively for Pb and Cd. The biosorption capacities were found as 34.3 mg g_1 for Pb and 37.1 mg g_1 for Cd. Pb and Cd concentrations in foods were determined. Surface microstructure was investigated by SEM–EDX.