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    Antioxidant, Antiglaucoma, Anticholinergic, and Antidiabetic Effects of Kiwifruit (Actinidia deliciosa) Oil: Metabolite Profile Analysis Using LC-HR/MS, GC/MS and GC-FID
    (Mdpi, 2023) Ozden, Eda Mehtap; Bingol, Zeynebe; Mutlu, Muzaffer; Karagecili, Hasan; Koksal, Ekrem; Goren, Ahmet C.; Alwasel, Saleh H.
    Determining the antioxidant abilities and enzyme inhibition profiles of medicinally important plants and their oils is of great importance for a healthy life and the treatment of some common global diseases. Kiwifruit (Actinidia deliciosa) oil was examined and researched using several bioanalytical methods comprehensively for the first time in this research to determine its antioxidant, antiglaucoma, antidiabetic and anti-Alzheimer's capabilities. Additionally, the kiwifruit oil inhibitory effects on acetylcholinesterase (AChE), carbonic anhydrase II (CA II), and alpha-amylase, which are linked to a number of metabolic illnesses, were established. Furthermore, LC-HRMS analysis was used to assess the phenolic content of kiwifruit oil. It came to light that kiwifruit oil contained 26 different phenolic compounds. According to the LC-HRMS findings, kiwifruit oil is abundant in apigenin (74.24 mg/L oil), epigallocatechin (12.89 mg/L oil), caryophyllene oxide (12.89 mg/L oil), and luteolin (5.49 mg/L oil). In addition, GC-MS and GC-FID studies were used to ascertain the quantity and chemical composition of the essential oils contained in kiwifruit oil. Squalene (53.04%), linoleoyl chloride (20.28%), linoleic acid (2.67%), and palmitic acid (1.54%) were the most abundant compounds in kiwifruit oil. For radical scavenging activities of kiwifruit oil, 1,1-diphenyl-2-picryl-hydrazil (DPPH center dot) and 2,2 '-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(center dot+)) radicals scavenging techniques were examined. These methods effectively demonstrated the potent radical scavenging properties of kiwifruit oil (IC50: 48.55 mu g/mL for DPPH center dot, and IC50: 77.00 mu g/mL for ABTS(center dot+) scavenging). Also, for reducing capabilities, iron (Fe3+), copper (Cu2+), and Fe3+-2,4,6-tri(2-pyridyl)-S-triazine (TPTZ) reducing abilities were studied. Moreover, kiwifruit oil showed a considerable inhibition effect towards hCA II (IC50: 505.83 mu g/mL), AChE (IC50: 12.80 mu g/mL), and alpha-amylase (IC50: 421.02 mu g/mL). The results revealed that the use of kiwifruit oil in a pharmaceutical procedure has very important effects due to its antioxidant, anti-Alzheimer, antidiabetic, and antiglaucoma effects.
  • [ X ]
    Öğe
    Hamamelitannin's Antioxidant Effect and Its Inhibition Capability on ?-Glycosidase, Carbonic Anhydrase, Acetylcholinesterase, and Butyrylcholinesterase Enzymes
    (Mdpi, 2024) Durmaz, Lokman; Karagecili, Hasan; Erturk, Adem; Ozden, Eda Mehtap; Taslimi, Parham; Alwasel, Saleh; Gulcin, Ilhami
    Hamamelitannin (2 ',5-di-O-galloyl-hamamelose) bears two-gallate moieties in its structure, and is a natural phenolic product in the leaves and the bark of Hamamelis virginiana. The antioxidant capacity of hamamelitannin was evaluated by a range of methods, with the following findings: the ability to reduce potassium ferric cyanide; the scavenging of N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD center dot+); the scavenging of 2,2 '-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS center dot+); the scavenging of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot); and the ability to reduce cupric ions (Cu2+). Additionally, reference antioxidants of alpha-Tocopherol, butylated hydroxyanisole (BHA), Trolox, and butylated hydroxytoluene (BHT) were used for comparison. For DPPH radical scavenging, hamamelitannin had an IC50 value of 19.31 mu g/mL, while the IC50 values for BHA, BHT, Trolox, and alpha-Tocopherol were 10.10, 25.95, 7.05, and 11.31 mu g/mL, respectively. The study found that hamamelitannin functioned similarly to BHA, alpha-tocopherol, and Trolox in terms of DPPH center dot scavenging, but better than BHT. Additionally, as a polyphenolic secondary metabolite, the hamamelitannin inhibition capability of several metabolic enzymes was demonstrated, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase I (CA I), carbonic anhydrase II (CA II) and alpha-glycosidase. The Ki values of hamamelitannin exhibited 7.40, 1.99, 10.18, 18.26, and 25.79 nM toward AChE, BChE, hCA I, hCA II, and alpha-glycosidase, respectively.