Yazar "Oto, Gokhan" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • [ X ]
    Öğe
    Antioxidant properties of Ferulago angulata and its hepatoprotective effect against N-nitrosodimethylamine-induced oxidative stress in rats
    (Taylor & Francis Ltd, 2017) Kiziltas, Hatice; Ekin, Suat; Bayramoglu, Mahire; Akbas, Esvet; Oto, Gokhan; Yildirim, Serkan; Ozgokce, Fevzi
    Context: Ferulago angulata (Schlecht.) Boiss. (Apiaceae) (FASB) is used to treat liver diseases and has been used both as food and therapeutics by many cultures for thousands of years because of the natural antioxidant compounds. Objective: This study determines antioxidant properties of FASB flowers, the levels of minerals and vitamins, and also, evaluates the hepatoprotective effect of flowers against N-nitrosodimethylamine (NDMA) induced on liver tissue by assessing antioxidant enzymes and histopathological parameters in Wistar albino rats. Materials and methods: In the study, the rats were divided into six groups of ten. Control, untreated animals were given 0.9% NaCl. Rats were intraperitoneally given NDMA (10 mg/kg) for the first 7 days. FASB methanol extract (150 and 300 mg/kg) was administered orally for 21 days. Results: a-Tocopherol, retinol, ascorbic acid, total antioxidant activity, phenolic and flavonoid contents of FASB were 0.70 +/- 0.13, 0.29 +/- 0.03 mu g/g, 139.32 +/- 7.06 mu g/100 g, 171.61 +/- 6.05 mM ascorbic acid/g, 90.47 +/- 4.11 mg GA/g and 37.39 +/- 2.85 mg QE/g. DPPH and hydroxyl radical scavenging activity was obtained IC 50 67.34 +/- 4.14 and 64.87 +/- 4.68 mu g/mL, respectively. Discussion and conclusion: The results of the study indicated that FASB flowers contain high levels of vitamins, minerals, total antioxidant activity, phenolics and flavonoids. Due to the positive effect on significant changes in antioxidant enzymes of liver tissue and histopathological examination, it is thought that the plant could be used as a hepatoprotective.
  • [ X ]
    Öğe
    Antioxidant properties of Rosa pisiformis and its protective effect against isoproterenol-induced oxidative stress in rats
    (Walter De Gruyter Gmbh, 2016) Bayramoglu, Mahire; Ekin, Suat; Kiziltas, Hatice; Oto, Gokhan; Susen, Ebru Altindal; Ozgokce, Fevzi
    Objective: In this study, Rosa pisiformis was evaluated for its antioxidant activity, vitamin (A, E, C), trace element (Fe, Cu, Zn, Mn, Cr, Se) and mineral (Ca, K, Mg, Na) levels, in addition to its cardioprotective effects on cardiac tissue antioxidant enzymes (GSH-Px, SOD, CAT) during isoproterenol (ISO)-induced oxidative stress. Methods: In the study, vitamin and mineral analysis was carried out using HPLC and ICP-MS method, R. P. antioxidant and antiradical properties were determined using spectrophotometer. Forty wistar albino rats were divided into four groups: control (0.9% NaCl), ISO (100 mg/kg), ISO (100 mg/kg) + R. P. (300 mg/kg) and R. P. (300 mg/kg). ISO was injected subcutaneously into rats twice at an interval of 24 h for two consecutive days (on 28(th) and 29(th) day). R. P. fruit administered daily. The experimental period was continued for 30 days. Results: The results of the study indicated that R. P. fruit and leaves contain high levels of minerals and vitamins and possess antioxidant and antiradical activity and its fruits have positive effects on cardiac tissues. The results are supported with significant changes in antioxidant enzyme levels and histopathological examination. Conclusion: This study demonstrates that R. P. fruits in particular can be used to treat and prevent cardiac diseases.
  • [ X ]
    Öğe
    Nephroprotective effect of Ferulago angulata flowers on N-nitrosodimethylamine-induced nephrotoxicity in rats and its phytochemical profile
    (Wiley, 2019) Ekin, Suat; Kiziltas, Hatice; Akkoyun, Mahire Bayramoglu; Ekin, Hasya Nazli; Yildirim, Serkan; Oto, Gokhan; Akbas, Esvet
    The present study was designed to assess the phytochemical content of Ferulago angulata (FA) and possible in vivo nephroprotective effect of FA administration on trace elements, minerals, MDA and GSH in kidney and liver tissue samples, serum vitamin (alpha-tocopherol, retinol, cholecalciferol, phylloquinone), TSA, and LSA in a rat model of DMN-induced nephrotoxicity. In the study, Wistar albino rats were assigned to six groups: Control (0.9% NaCl), (DMN 10 mg/kg), (FA 150 mg/kg), (DMN + FA 150 mg/kg), (FA 300 mg/kg), and (DMN + FA 300 mg/kg). Rats were intraperitoneally given DMN for the first 7 days. Renal injury caused by DMN was proved by the histopathological alterations. The FA (300 mg/kg) treatment significantly normalized Se, Cr, Ca levels in liver and Co level in kidney tissue samples. These observed positive effects are due to the phytochemical content of the plant. The flower extract of FA (300 mg/kg) can be used for the prevention of kidney damage. Practical applications Ferulago angulata flowers are used in traditional medicine for treat kidney and liver digestive system diseases. This species is endemic taxa of the family Apiaceae, which has been used both as food and therapeutics because of their phytochemical composition. In this study, the phenolic characterization of FA flower was used to a new RP-HPLC method, as well as the biological activity of FA flower and possible in vivo nephroprotective effect of FA flowers on trace elements, minerals, MDA and GSH in kidney and liver tissue samples and vitamins, TSA, and LSA in serum samples a rat model of DMN-induced nephrotoxicity. It was found that high level of phenolic compounds (chlorogenic acid, vanillic acid, 2-hydroxycinnamic acid) present in the flower extract of F. angulata has positive effects and antioxidant properties. Due to its phenolic content, FA flower extract could protect for kidney damage and can be used as antioxidants in the food additive and pharmaceutical industry.
  • [ X ]
    Öğe
    Phenolic Contents, Enzyme Inhibitory Activities, and Protective Effect of Aqueous Extract of Rosa Pisiformis Fruits
    (Springer, 2024) Ekin, Suat; Akkoyun, Mahire Bayramoglu; Kiziltas, Hatice; Gok, Hasya Nazli; Oto, Gokhan; Akkoyun, Turan; Ozgokce, Fevzi
    The present study was designed to assess the phytochemical contents as well as the in vitro biological activities fruit of Rosa pisiformis and possible in vivo cardioprotective and hepatoprotective properties of the aqueous extract of R. pisiformis fruit on trace elements (cadmium, selenium, nickel, zinc, iron, copper, cobalt, chromium, manganese, lead), minerals (sodium, calcium, potassium, magnesium), glutathione and malondialdehyde in heart and liver tissue samples, serum vitamin (retinol, cholecalciferol, phylloquinone, alpha-tocopherol), total sialic acid, and lipid-bound sialic acid in a rat model of isoproterenol-induced oxidative damage. In the study, 40 Wistar albino rats were divided into four groups of ten each: control, isoproterenol 100 mg/kg bodyweight; isoproterenol 100 mg/kg bodyweight; then R. pisiformis 300 mg/kg bodyweight, and R. pisiformis 300 mg/kg bodyweight. Rats were given isoproterenol twice at an interval of 24 h for two days (on days 28 and 29) subcutaneously. The experimental period was maintained at 30 days. According to analysis results, caffeic acid and p-coumaric acid were found to be the high contents of the fruit extracts at 6.01 +/- 0.0006 and 3.93 +/- 0.007 mg/100 g dry weight. It showed that R. pisiformis (300 mg/kg bodyweight) aqueous extract had a potent action on oxidative damage. The R. pisiformis (300 mg/kg bodyweight) treatment significantly alleviated toward normalcy on the zinc, manganese, cobalt, magnesium, and sodium values in the heart, and zinc and magnesium values in liver tissue samples. These positive effects may be related to the action of p-coumaric acid and caffeic acid present in the R. pisiformis 300 mg/kg and it has hypolipidemic and antioxidant properties that could protect from myocardial damage.
  • [ X ]
    Öğe
    Theophylline attenuates bleomycin-induced oxidative stress in rats: The role of IL-6, NF-KB, and antioxidant enzymes
    (Univ Sao Paulo, Conjunto Quimicas, 2022) Ekin, Suat; Yildirim, Serkan; Akkoyun, Mahire Bayramoglu; Gok, Hasya Nazli; Arihan, Okan; Oto, Gokhan; Akkoyun, Turan
    The purpose of this study was to evaluate the antifibrotic and antioxidant roles of theophylline (Theo), a bioactive compound, in bleomycin (BLM)-induced pulmonary fibrosis in Wistar albino rats. Assigned into 4 groups were 32 Wistar albino rats, comprising the control group (administered 0.9% isotonic saline), BLM group (treated with BLM at a dose of 2.5 mg/kg), BLM+Theo group (treated with Theo at a dose of 75 mg/kg + BLM at a dose of 2.5 mg/kg), and Theo group (treated with Theo at a dose of 75 mg/kg). In the BLM group, a significant decrease was observed in the catalase and glutathione peroxidase enzyme activities, and reduced glutathione (GSH) (p < 0.05, p < 0.05, p < 0.001, respectively), while the malondialdehyde (MDA) levels (p < 0.001) were significantly elevated when compared to the control group. However, the MDA levels in the BLM+Theo group were also significantly higher than in the control group (p < 0.01). Similarly, the GSH levels were significantly higher in the BLM+Theo group than in the BLM group (p < 0.05). The results indicated that Theo reduced the BLM-induced activation of nuclear factor-kappaB (NF-kappa B) and decreased interleukin-6 (IL-6) levels, together with significant amelioration of the immunohistochemical and histopathological architecture in the lung tissues. It was concluded that the administration of Theo had a positive effect on the GSH level, and activation of NF-kappa B and IL-6 expression, which were significant proinflammatory markers in the BLM-treated rats.