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    Öğe
    Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources
    (Galenos Publ House, 2022) Karatas, Merve Dilek; Hazrati, Nahid; Oguz, Ezgi; Ozmen, Canan Yuksel; Altintas, Serdar; Akcay, M. Emin; Ergul, Ali
    Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes (alleles). Pear (Pyrus communis L.) belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Ataturk Horticultural Central Research Institute-Yalova collection is considered as genes bank. Identification of the different features of this collection (fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.) is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR (Polymerase Chain Reaction)-based amplification of the S-allele regions (S-1, S-6, S-7, S-8) causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S-6 allele was the most prevalent one with 63% frequency, while the S-8 allele was the least common allele with a rate of 4%. In allele combinations, the SI-So allele combination was the most common allele combination with a rate of 18%. and trilateral allele combinations (S-1-S-6-S-7 and S-1-S-6-S-8) were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.
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    Öğe
    The Comparison of Regeneration from Root Node Explants in Solanaceae
    (Hard, 2021) Oguz, Muhammad Cagri; Karata, Merve Dilek; Oguz, Ezgi; Mujtaba, Muhammad; Altinta, Serdar; Ergul, Ali
    Tissue culture techniques in tomato, pepper and eggplant are important for the development of disease-resistant and high yielding varieties, which require a suitable regeneration protocol. Although shoot regeneration has been achieved by using different explants and cytokinin doses in Solanaceae species, very few studies have reported in vitro regeneration using root tissues. The current study is the first report to compare direct shoot regeneration capabilities using root node explants in three Solanaceae species (tomato, pepper, and eggplant) under three cytokinins (BAP, TDZ, and GA3) hormone. Plantlets were regenerated from the root node explants of tomato, pepper and eggplant in the media containing 0, 0.5, 1, 1.5 and 2mg/L of BAP, TDZ and GA3. Results revealed that the shoot regeneration of root node explants varied according to the species, cytokinins (BAP, TDZ and GA3) and doses of hormones. Among the species, the best shoot regeneration was observed in tomato followed by eggplant and pepper plants. While the shoot length was statistically significant in tomato, it was observed to be insignificant in pepper and eggplant. The highest number of root regeneration and root length was observed in tomatoes. The results obtained from the study will contribute to the development of successful/reproducible tissue culture protocols from roots node explants.