Yazar "Mirzapour, Mohsen" seçeneğine göre listele

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    Bitki Gelişimini Tetikleyen Rizobakterilerin Uygulandığı Buğdayda (Triticum aestivum L.) Kuraklık Stresi ileİlişkili Bazı Genlerin İfade Seviyesinin Ölçülmesi
    (2021) İnal, Behçet; Bektaş, Harun; Mirzapour, Mohsen; Altıntaş, Serdar; Çığ, Fatih; Cengiz, Mustafa; Sonkurt, Mehmet
    Ekmeklik buğday (Triticum aestivum L.) temel besin kaynağı olması ve tüm dünyada üretimi yapılabilen bir ürünolması nedeniyle sürdürülebilir tarım açısından en önemli bitki türlerinden biridir. Buğday bitkisinin genetik yapı olarakmısır, çeltik ve diğer tüm tarımsal ürünlerden daha kompleks bir yapıya sahip olması bu türün ıslahını zor ve uzun zamanalan bir süreç haline getirmektedir. Diğer taraftan verim değerlerinin istenilen noktalara getirilebilmesi buğdayın çevreselfaktörlere verdiği tepkilerin anlaşılması ile mümkün olabilmektedir. Bu çalışmada da buğday ıslahında en sık karşılaşılansorunlardan kuraklık ve hastalıklara karşı direncin ACC deaminaz etkisi gösteren PGPB (Plant Growth Promoting Bacteria)ile ilişkisi incelenmiştir. Çalışmamızda ACC deaminaz sentezleyen bakterilerin iki farklı ekmeklik buğday çeşidinde (Gerek79, Bezostaja 1) ve kuraklık koşullarındaki etkisi incelenmiştir. Çalışma ile ACC deaminaz etkisi ile kuraklığa karşıdayanıklılık mekanizmasında rol alan bazı transkripsiyon faktörlerin ifade seviyeleri q-RT PCR ile ölçülmüştür. Ayrıca heriki buğday genotipinde glutatyon redüktaz seviyesi ölçülerek genler ile olan ilişkisi ortaya konulmuştur. Çalışma sonucundaelde edilen veriler değişen etkinlik derecesine sahip olmakla birlikte PGPB bakterilerinin kuraklık stresinin olumsuzetkilerini azaltıcı etkiye sahip olduğu bulunmuştur.
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    Öğe
    BREAKING SEED DORMANCY AND MICROPROPAGATION OF PERENNIAL VULNERARIA MILKVETCH (Astragalus vulnerariae DC.)
    (Wydawnictwo Akad Rolniczej W Lublinie, 2017) Dilaver, Zuhal; Mirzapour, Mohsen; Kendir, Hayrettin
    In this study, an efficient system to break seed dormancy and plant regeneration was established for perennial vulneraria milkvetch (Astragalus vulnerariae DC.). The seed coat dormancy could be easily released using 40% sulphuric acid treatment for 15 minutes, which made the seed coat permeable without damaging zygotic embryos. The tissue culture studies reported regeneration on 5 explants, which had variable effects on shoot regeneration, using MS medium containing variable concentrations of Kin-NAA and BAP-NAA. Maximum shoot regeneration of 86.67% with 4.47 shoots per explant was noted on MS medium containing 0.5 mg center dot l(-1) Kin -0.5 mg center dot l(-1) NAA on hypocotyl explants. No shoot regeneration was noted on cotyledon leaf and shoot nodes using MS medium containing any concentration of BAP-NAA. Maximum shoot regeneration of 50% with 3 shoots per explant was also observed on 1 mg center dot l(-1) BAP-0.5 mg center dot l(-1) NAA on epicotyl explants. MS medium containing Kin-NAA induced hypocotyl explant shoots had rooting percentage of 36.67% on 0.5 mg center dot l(-1) IBA, whereas, MS medium containing BAP-NAA induced hypocotyl shoots had rooting percentage of 46.67%. The in vitro cultured plants had an acclimatization rate of 78%. Aesthetically attractive, economical, easy to maintain and water efficient vulneraria milkvetch is currently not available in landscaping. The system of plant regeneration and adaptation suggested by this paper may help to dissipate it to a broad range of water scarce environments as a sustainable and inexpensive choice.
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    Öğe
    Drought-Induced miRNA Expression Correlated with Heavy Metal, Phenolic Acid, and Protein and Nitrogen Levels in Five Chickpea Genotypes
    (Amer Chemical Soc, 2023) Inal, Behcet; Mirzapour, Mohsen; Tufekci, Ebru Derelli; Rustemoglu, Mustafa; Kaba, Adem; Albalawi, Marzough Aziz; Alalawy, Adel I.
    Drought is a prime stress, drastically affecting plant growth, development, and yield. Plants have evolved various physiological, molecular, and biochemical mechanisms to cope with drought. Investigating specific biochemical pathways related to drought tolerance mechanisms of plants through biotechnology approaches is one of the quickest and most effective strategies for enhancing crop production. Among them, microRNAs (miRNAs) are the principal post-transcriptional regulators of gene expression in plants during plant growth under biotic and abiotic stresses. In this study, five different chickpea genotypes (I?nci, Hasan bey, Arda, Seckin, and Diyar 95) were grown under normal and drought stress. We recorded the expression levels of microRNAs in these genotypes and found differential expression (miRNA396, miR408, miRNA414, miRNA528, and miRNA1533) under contrasting conditions. Results revealed that miRNA414 and miRNA528 considerably increased in all genotypes under drought stress, and expression levels of miRNA418, miRNA1533, and miRNA396 (except for the Seckin genotype) were found to be higher under the watered conditions. These genotypes were also investigated for heavy metal, phenolic acid, protein, and nitrogen concentrations under normal and drought stress conditions. The Arda genotype showed a significant increase in nitrogen (5.46%) and protein contents (28.3%), while protein contents were decreased in the Hasan bey and Seckin genotypes subjected to drought stress. In the case of metals, iron was the most abundant element in all genotypes (I?nci = 15.4 ppm, Hasan bey = 29.6 ppm, Seckin = 37.8 ppm, Arda = 26.3 ppm, and Diyar 95 = 40.8 ppm) under normal conditions. Interestingly, these results were related to miRNA expression in the chickpea genotypes and hint at the regulation of multiple pathways under drought conditions. Overall, the present study will help us to understand the miRNA-mediated regulation of various pathways in chickpea genotypes.
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    Öğe
    Impact of Different Fertilizer Sources under Supplemental Irrigation and Rainfed Conditions on Eco-Physiological Responses and Yield Characteristics of Dragon's Head (Lallemantia iberica)
    (Mdpi, 2023) Heydarzadeh, Saeid; Arena, Carmen; Vitale, Ermenegilda; Rahimi, Amir; Mirzapour, Mohsen; Nasar, Jamal; Kisaka, Oscar
    The effects of the irrigation regime and different fertilizer sources on the eco-physiological responses and yield characteristics of dragon's head were explored in a factorial experiment based on a randomized complete block design with 12 treatments and 3 replications in the 2019 growing season. The treatments included six different fertilizer sources (animal manure, vermicompost, poultry manure, biofertilizer, chemical fertilizer, and control) and two irrigation regimes (rainfed and supplemental irrigation). The results indicated the positive effects of supplementary irrigation and the application of vermicompost, poultry manure, and animal manure by increasing the absorption of nutrients (phosphorus and potassium) and improving relative water contents, chlorophyll and carotenoid contents, and the fixed oil percentage of dragon's head. The activities of catalase, ascorbate peroxidase, and superoxide dismutase decreased in the rainfed plants, whereas organic fertilizer application increased the antioxidant enzyme activity. The highest grain yield (721 kg ha(-1)), biological yield (5858 kg ha(-1)), total flavonoids (1.47 mg g(-1) DW), total phenol (27.90 mg g(-1) DW), fixed oil yield (200.17 kg ha(-1)), and essential oil yield (1.18 kg ha(-1)) were noted in plants that were treated with vermicompost under supplemental irrigation. Therefore, it is recommended that organic fertilizers such as vermicompost and poultry manure be used to substitute chemical fertilizers. These practices can help popularize organic crops using rainfed and supplementary irrigation.
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    Mitigation of Drought Stress in Wheat by Bio-priming by PGPB Containing ACC Deaminase Activity
    (2022) Çığ, Fatih; Erman, Murat; İnal, Behçet; Bektaş, Harun; Sonkurt, Mehmet; Mirzapour, Mohsen; Ceritoğlu, Mustafa
    Out of stress management strategies used for drought, inoculation of plant growth-promoting bacteria holds a major position due to sustainable, low-cost, and versatile properties. The plant growth-promoting bacteria, particularly containing 1-aminocyclopropane-1-carboxylic acid deaminase activity, have a critical location since they restrict ethylene synthesis under stress conditions thereby improving stress tolerance index. In this experiment, seeds of two wheat cul- tivars were primed with three bacterial strains and seedlings were grown under stress and non- stress conditions. The study was laid out in completely randomized factorial design with three replications. While plant growth achieved top performance with synthetic fertilizer in 80% of field capacity, increasing drought stress restricted the efficiency of synthetic fertilizer. In contrast, plant growth-promoting bacteria-priming promoted plant growth and dry matter accumulation under optimum and drought conditions. Increase of dry matter accumulation in treatments as con- trol plants varied between 17.1% and 57.1% under 80% of field capacity while it changed between 0.2% and 35.1% under drought conditions. TV126C and TV24C induced stress tolerance index in sensitive and tolerant cultivars under drought and optimum conditions. In conclusion, it is con- sidered that bio-priming with plant growth-promoting bacteria involving 1-aminocyclopropane- 1-carboxylic acid deaminase enzyme activity might be an effective and sustainable management strategy to drought stress in wheat cultivation.