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    Microscopic and Molecular Prevalence of Cryptosporidium spp. in Lambs in Siirt, Turkey
    (Natl Information Documentation Centre, 2023) Celik, Ozguer Yasar; Celik, Burcak Aslan; Ayan, Adnan; Kilinc, Oezlem Orunc; Ercan, Kerem; Selcuk, Muhammed Ahmed; Baldaz, Vedat
    CRYPTOSPORIDIOSIS is a zoonotic disease that occurs acutely or chronically in young or immune-compromised animals and humans, caused by Cryptosporidium species. Cryptosporidium is recognized as one of the major enteropathogens associated with neonatal diarrhea in ruminants. The aim of this study is to determine the prevalence of Cryptosporidium spp. in lambs in Siirt province using microscopic and molecular methods. The fecal materials of the study collected from 194 randomly selected lambs of different sexes, up to 4 weeks of age, in various farms. As a result of microscopic examination, Cryptosporidium spp. oocysts were found in 54 (27.84%) of 194 samples, while specific bands were obtained in 63 (32.47%) samples as a result of Nested PCR analysis. A statistically significant relationship was found between lambs with and without diarrhea (P<0.001) while no statistically significant relationship was found between age groups, gender, and locations (P>0.05). In conclusion, the data obtained from this study revealed that Cryptosporidium infection is present in Siirt province, and Cryptosporidium spp. should be considered as one of the agents in the etiology of neonatal diarrhea in lambs.
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    Molecular Prevalence of Giardia duodenalis and Subtype Distribution (Assemblage E and B) in Calves in Siirt, Turkey
    (Natl Information Documentation Centre, 2023) Celik, Burcak Aslan; Celik, Ozguer Yasar; Ayan, Adnan; Akyildiz, Guerkan; Kilinc, Oezlem Orunc; Ayan, Oezge Oktay; Ercan, Kerem
    GIARDIA duodenalis is a common intestinal protozoan parasite that infects domestic and wild mammals, birds, and humans, especially livestock, worldwide. Studies on the prevalence of Giardia duodenalis in calves in Turkey are very limited. The aim of this study was to determine the molecular prevalence and subtype distribution of Giardia duodenalis in calves in Siirt. The animal material of the study consisted of 100 calves of different breeds and sexes in Siirt province. Using a disposable latex glove, feces samples were taken from the rectum of each calf and put in separate fecal containers. Sex and age information was recorded for each sample collected. As a result, 5% and 9% positivity was detected by microscopic and nested-PCR methods, respectively. The prevalence was 9.68% in females and 7.89% in males (P>0.05). Prevalence by age groups was as follows: 11.32% in the 1-6 month group, 7.41% in the 7-12 month group, and 5% in the older than 12 months group (P>0.05). As a result of the sequence analysis of the five PCR-positive samples, 80% Assemblage E and 20% Assemblage B were detected. As a result of this study, in addition to Assemblage E, Assemblage B was detected as well, which also has zoonotic properties. This situation may pose a risk for breeders. To better understand the distribution of G. duodenalis assemblages in calves in Siirt, studies in larger herds are needed.