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    A SURVEY OF TOXOCARA VITULORUM IN ANATOLIAN WATER BUFFALOES (BUBALIS BUBALIS) IN DIYARBAKIR, TURKEY
    (Assiut University, Faculty of Veterinary Medicine, 2022) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Kilinç, Özlem Orunç; Ayan, Özge Oktay; Görmez, Gül
    Toxocara vitulorum is a pathogenic gastrointestinal nematode found in cattle and buffaloes all over the world, especially in tropical and subtropical regions with humid climates. Buffalo farming is a sector whose value has increased in recent years in Turkey and is intensively performed to obtain various products such as milk, cream, meat, and horns. This study aims to determine the prevalence of T. vitulorum in Anatolian Water Buffaloes in Diyarbakır. Fresh stool samples from animals were placed in individual stool containers. The sex and age of the animal were recorded for each sample collected. The samples were evaluated under the light microscope after the application of the Fulleborn saturated salt solution method and under Scanning Electron Microscope. Positivity was detected in 5 (3.01%) of the 166 samples. Regarding prevalence by age groups, T. vitulorum was detected in 10% of the 0-6 month group, 4.17% of the 6-12 month group, and 0.89% of the older than 12 months group. The prevalence by sex was determined as 3.85% for females and as 1.61% for males. To determine the epidemiology of the disease in the region, it was concluded that larger herds should be studied and serological and molecular methods should be used to confirm and support the findings of microscopic methods. © 2022, Assiut University, Faculty of Veterinary Medicine. All rights reserved.
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    MOLECULAR INVESTIGATION OF SOME BACTERIA (COXİELLA BURNETİİ, MYCOPLASMA HAEMOCANİS, CANDİDATUS MYCOPLASMA HAEMATOPARVUM, WOLBACHİA) IN RHIPICEPHALUS SANGUINEUS TICKS IN SIIRT PROVINCE, TURKEY
    (Assiut University, Faculty of Veterinary Medicine, 2022) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Yilmaz, Ali Bilgin; Kilinç, Özlem Orunç; Şababoğlu, Ezgi; Ayan, Özge Oktay
    Ticks harbor the largest diversity of microorganisms, ranging from viruses, prokaryotes, and eukaryotes. Rhipicephalus sanguineus ticks are the most common ticks worldwide. Although dogs are the main host of this tick species, it has been reported that it also infests humans in various parts of the world. This study aimed to examine some bacteria (Coxiella burnetii, Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Wolbachia) in Rhipicephalus sanguineus ticks sampled from dogs. In this study, 350 tick samples collected from 85 dogs in Siirt province were determined to be Rhipicephalus sanguineus ticks. Coxiella DNA was detected in 3 (0.85%) out of 350 ticks using Nested PCR (687 base pairs). None of the samples were found to contain Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, and Wolbachia DNA. A partial sequence of the IS1111 gene region was registered in GenBank with OM472143 accession numbers. Considering the zoonotic nature of the Q disease, it is very important for dog owners and related institutions to periodically spray animals against ticks, and to take any other necessary precautions. More samples are needed to determine the Mhc, CMhp, and Wolbachia prevalence. © 2022, Assiut University, Faculty of Veterinary Medicine. All rights reserved.