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    A novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis
    (Elsevier Sci Ltd, 2020) Dolak, Ibrahim; Canpolat, Gurbet; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, Arzu
    Purification and analyzing of proteins is an essential means for understanding their function and diseases associated with their lack or defect. In this research, a new lanthanide-chelate based molecularly imprinted polymer (MIP) was synthesized for selective separation of Hemoglobin (Hb) from human serum in the presence of various interference molecules. The Hb-imprinted polymer was prepared by using complex functional monomer N-methacryloylamido antipyrine (MAAP)-Ce(III) and 2-Hydroxyethyl methacrylate (HEMA) in accordance of cryopolymerization techniques. The nonimprinted cryogel (NIP) was also prepared at same polymerization conditions in the absence of template Hb molecule. The effects of pH, initial Hb concentration, flow rate, temperature and ionic strength on the binding capacity of both imprinted and nonimprinted cryogels was investigated. The maximum binding capacity for the MIP column was found to be as 79.41 mg g(-1) dry cryogel, that is four times higher than the NIP column under the optimum conditions (pH 5.0, flow rate: 1.0 mL min(-1), T: 25 degrees C). Moreover, selectivity experiments were performed by using two interference proteins as myoglobin (Mb) and cytochrome c (Cyt-c) and the relative selectivity coefficients (k') for Hb/Mb and Hb/Cyt-c pairs were determined as 36.59 and 37.22, respectively.
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    Conductive Polymer-Based Nanocomposites as Powerful Sorbents: Design, Preparation and Extraction Applications
    (Taylor & Francis Inc, 2022) Canpolat, Gurbet; Dolak, Ibrahim; Kecili, Rustem; Hussain, Chaudhery Ghazanfar; Amiri, Amirhassan; Hussain, Chaudhery Mustansar
    Conductive polymers as composite materials have been attracted tremendous attention due to their versatile and excellent features such as tunable conductivity, facile synthesis and fabrication, high chemical and thermal stability etc. These characteristics make them versatile and let them being used in numerous fields including microelectronics, optics and biosensors. Throughout the mentioned fields, conductive polymers particularly perform as effective sorbents. Although tremendous efforts have been put into this topic, to the best of our knowledge, a comprehensive up-to-date review on the applications of conductive polymers as efficient sorbents has not been reported. The main objective of this paper is to make a significant contribution to the recent literature toward the synthesis and extraction applications of conductive polymers as efficient sorbents.
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    Development of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator
    (Elsevier Sci Ltd, 2021) Canpolat, Gurbet; Dolak, Ibrahim; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, Arzu
    The recognition and detection of proteins has an important role in the fields of biochemistry and medicine in term of diagnosis and prognosis. In this study, molecularly imprinted cryogel (MIP) was synthesized for the selective recognition of Cytochrome-c (Cyt-c) by cryopolymerization techniques with the lanthanide-chelate approach using (MAAP)2-Ce(III) as complex functional monomer and Cyt-c as a template protein. The incorporation of template with monomer was characterized by near- infrared (Near-IR) spectroscopy. The binding capacity was optimized in accordance with numerous experimental parameters including pH, initial Cyt-c concentration, flow rate, temperature and ionic strength. As a result, the maximum binding capacity reached to 98.33 mg g-1 in a buffer system with a pH of 6.0. Beside of the excellent reusability performance of prepared MIP, it was successfully applied to separation of template Cyt-c in the existence of hemoglobin (Hb) and myoglobin (Mb) chosen as interfering proteins. The relative selectivity constants of the Cyt-imprinted polymer were determined as 13.84 for the Cyt/Mb pair and 16.05 for the Cyt/Hb pair. Consequently, a high selectivity, simple and cheap strategy for efficient separation of proteins was provided with this novel MIP cryogel.
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    Functionalized magnetic nanoparticles as powerful sorbents and stationary phases for the extraction and chromatographic applications
    (Elsevier Sci Ltd, 2021) Kecili, Rustem; Ghorbani-Bidkorbeh, Fatemeh; Dolak, Ibrahim; Canpolat, Gurbet; Karabork, Muharrem; Hussain, Chaudhery Mustansar
    Materials in nanodimensions such as magnetic nanoparticles exibit fascinating physico-chemical features (i.e. high surface area, very small size, superparamagnetism, active surface which can be successfully functionalized, low toxicity, etc). Because of these superior features, functionalized magnetic nanoparticles received increasing attention for tremendous amount of studies over the last years and a number of successful applications were published. This paper aims to present and highlight the latest progresses in the development of novel functionalized magnetic nanoparticles for the extraction and chromatographic applications. (C) 2021 Elsevier B.V. All rights reserved.