Yazar "Kaya, Mustafa Oğuzhan" seçeneğine göre listele

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  • Küçük Resim
    Öğe
    Bazı yem ve gübre sanayi kimyasallarının hyalüronidaz üzerine in vitro etkileri
    (2016) Kaya, Mustafa Oğuzhan; Yerlikaya, Emrah; Özyazıc, Mehmet Arif; İrak, Kıvanç
    ABSTRACT This study investigates in vitro effects of some chemicals, used as a spreading factor on therapeutic and various medical fields and applied frequently in forage and fertilizer industry on bovine testicular hyaluronidase (BTH) which is substantially effective in glucotechnological applications and delivery of artificial insemination. BTH was purified through ammonium sulfate precipitation method and affinity gel (Sepharose-4B-L- tyrosine -m-anisidine). The inhibiton effects of Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O and indole-butyric acid were determined on purified BTH. IC50 values of these chemicals were found as 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM respectively and at the end of research, indole-butyric acid (IBA) was found as the strongest inhibitory effect compound by the value of IC50 with 4.265×10-2 mM.
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    Öğe
    Carbonic Anyhdrase Enzyme From The Siirt Mohair Goat Liver : Purification, Characterization and Assessment of Enzyme Kinetics Against Metal Toxicity
    (2017) Yerlikaya, Emrah; Karageçili, Hasan; Demirdağ, Ramazan; Kaya, Mustafa Oğuzhan
    Because of their physiological and clinical roles, carbonic anhydrases (CAs) are the most studied enzymes. In earlier studies; CA enzymes have been purified and characterized from the tissues and erythrocytes of many organisms such as; dog, swine, sheep, chicken, bee, fish, bovine, bacteria and human. In this study, the CA enzyme has purified from Siirt Mohair Goat liver tissue with 1930.84 EU.mg-1 of specific activity, 57.28% of purification yield and 80.55 of purification folds. The purity of the purified enzyme has confirmed by SDS-PAGE. As the characterization of CA enzyme’s in Siirt Mohair Goat liver has been done; the optimum ionic strength: 25 mM, the optimum pH: 8.0, the optimum temperature: 40ºC and the stable pH: 7.0 has been determined. Inhibitory effects of some metal ions have been examined on the purified CA enzyme. IC50 values of inhibiting metal ions were found as 2.24, 2.76, 2.36, 3.20, 2.55, 2.25, 3.28, 2.13, 3.10, 1.75, 2.16 and 3.50 mM for Al3+, Ni2+, Cd2+, Cu2+, Pb2+, Ba2+, Zn2+, B3+, Fe3+, Se2+, Ag+ and Co2+ respectively. As a result, CA enzyme was first purified from the Siirt Mohair Goat liver and the characteristics of the enzyme were investigated in this study.
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    Comparison of biochemical parameters of prevalent hemoglobinopathies with healthy individuals
    (2020) Yerlikaya, Emrah; Karageçili, Hasan; Kaya, Mustafa Oğuzhan
    Thalassemia is the most frequently seen monogenetic disorders around the world that is inherited as a recessive single-gene disease, resulting from mutations in ?-or?-globin gene clusters. In this study Beta Thalassemia Intermedia, Beta Thalassemia Minor, Sickle Cell Disease patients and the healthy control individuals both incidence and serum parameters were examined and analyzed retrospectively between 2015-2017 from Public Health information system. Beta Thalassemia Intermedia, BetaThalassemia Major, Beta Thalassemia Minor and Sickle Cell Disease patient groups incidences were evaluated on the basis of their HPLC analysis. Patients and controlgroups serum parameters levels were analyzed from their autoanalyzer results. These serum biochemical tests datas were controlled and taken from Siirt Public HealthCentre information system. In each year, there were recorded approximately 12 Beta Talassemia patients, 2 Beta Thallassemia Major patients, 340 Beta ThalassemiaMinor patients and 33 Sickle Cell Disease patients.Beta Thalassemia Intermedia patients serum parameters levels were compared with Beta Thalassemia Minor Patientsserum parameters and control group serum levels. There were seen statisticaly significant difference between Beta Thalassemia Intermedia patient’s serum ast, albumin,creatine, amylase, phosphor, iron, iron binding capacity, total protein, sodium, bilirubin direct, bilirubin total, LDH level and control serum parameters levels, p<0.05. Butthere were not seen statistically significant differences between Beta Thalassemia Minor patients serum levels and control serum levels. Studies like this should be donenationwide, in each city to determine the current state of abnormal hemoglobinopathies in Turkey. Thus, a broader, comprehensive national screening program is needed.
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    Öğe
    Determination of Antimicrobial and Antioxidant Activities of Lavandula angustifolia Volatile Oil
    (2022) Özdemir, Oğuzhan; Yılmaz, Nurten; Gök, Mesut; Kaya, Mustafa Oğuzhan
    This study aimed to evaluate the antimicrobial effect of the oil extracted from Lavandula angustifolia (Province of Denizli/Türkiye) on some gram-negative/positive pathogenic microorganisms and lactic acid bacteria (by agar well diffusion, MIC test), and antioxidant capacity (DPPH, metal chelating, FRAP). As a result of the analysis, it was determined by headspace/GC-MS analysis that eucalyptol was the highest with a value of 22.58%, ?-linalool was the second with a value of 19.44% and linalyl acetate was 14.61%. Lavandulol, an essential component of Lavender, was determined by 1.39%. The highest antimicrobial effect of L. angustifolia oil was found on Bacillus subtilus and Methicillin-resistant Staph. aureus (MRSA) with zone diameters of 24 to 22 mm, respectively), followed by Staph. aureus. Antibacterial effects of L. angustifolia oil on Staph. aureus, E. coli, Campliobacter jejuni, Salmonella poona, Lactobacillus reuteri and Lactobacillus plantarum were detected as 17, 16, 14, 14, 12, 12, and 11 mm zone diameters, respectively. Therewithal, a significant antioxidative effect was determined by DPPH, FRAP, and metal chelating analyzes.
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    Romanov Koyunları Amniyon Sıvısı ile Hyalüronidaz Aktivitesinin Araştırılması
    (2018) Kaya, Mustafa Oğuzhan; İrak, Kıvanç; Mersin, Seyrani; Yerlikaya, Emrah; Karageçili, Hasan
    Sığır testis hyalüronidazı (Bovine Testicular Hyaluronidase, BTH) endüstriyel alanda, kanser çalışmalarında ve birçokhayvan uygulamalarında oldukça önemli bir enzimdir. Bu çalışmada, Romanov ırkı koyunların amniyon sıvıları BTH enzimiiçin alternatif yeni bir substrat kaynağı olarak kullanılıp kullanılamayacağı araştırılmıştır. Araştırma sonucunda, ticari olaraksatın alınan hyalüronik asit (HA) ile tespit edilen BTH aktivitesi 9.187 U mL -1 bulunurken, 35 farklı amniyotik kesedenalınan sıvılar kullanılarak BTH aktivitesi 1.604-18.659 U mL -1 aralığında bulunmuştur. Romanov ırkı koyunların amniyonsıvısının HA içeriğinden dolayı BTH enzimi için bir substrat kaynağı olup olmadığına dair rapor edilen herhangi bir bilgiyerastlanmamıştır. Çalışma sonunda ticari HA substrat olarak kullanıldığında hesaplanan BTH aktivitesi ile amniyon sıvılarıkullanılarak elde edilen BTH aktiviteleri oldukça yakın olduğundan, Romanov ırkı koyunların amniyon sıvılarının yeni birHA kaynağı olabileceği düşünülmüştür.
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    Öğe
    The Prevalence of Neoplasm Diseases and Investigation of Some Biochemical Serum Parameters
    (2021) Karageçili, Hasan; Yerlikaya, Emrah; Kaya, Mustafa Oğuzhan
    Aim: The aim of this work, investigating the skin, lung, and bone marrow-related cancer prevalence and biochemical serum parameters of all these cancer patients. Materials and Methods: The skin, lung, and bone marrow cancer patient's biochemical serum data were examined retrospectively using a hospital information system. Results: Basal cell carcinoma patients number were recorded as 155 with 63%, squamous cell carcinoma patients number were enrolled in73 with 30% and malign melanoma patients number were noted as 10 with 4%. Skin cancers were recorded to be the most seen cancer type in 246 patients with 22% between 2013-2017 years. All types of cancer patient's numbers were calculated as 1134 between these years. The very common incidences of basal cell carcinoma and squamous cell carcinoma cancers were observed on the upper part of the body, respectively on the face-cheek, nose, ear, eyelid, and lip. In this study, there was seen a statistically significant difference between skin cancer patient’s serum glucose, aspartate aminotransferase, alanine aminotransferase, sodium, bilirubin direct, bilirubin total, creatinine, urea level, and control group serum parameters level, p<0.05. The lung cancer patient numbers were recorded as 119 with 10.4 %, bone marrow patients numbers were enrolled in 113 with 10%. Two cancer groups were statistically different in terms of 5-years survival. Log Rank X2 = 8.68, p = 0.003, p<0.05. Conclusions: We recorded that skin cancer types and regions on the upper parts of the body were more because of exposure to the sun. The lung cancer survival rate was lower than bone marrow cancer. Moreover, we strongly emphasized that measuring the biochemical serum parameters was statistically significant in the diagnosis of cancer patients.