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    Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources
    (Galenos Publ House, 2022) Karatas, Merve Dilek; Hazrati, Nahid; Oguz, Ezgi; Ozmen, Canan Yuksel; Altintas, Serdar; Akcay, M. Emin; Ergul, Ali
    Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes (alleles). Pear (Pyrus communis L.) belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Ataturk Horticultural Central Research Institute-Yalova collection is considered as genes bank. Identification of the different features of this collection (fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.) is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR (Polymerase Chain Reaction)-based amplification of the S-allele regions (S-1, S-6, S-7, S-8) causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S-6 allele was the most prevalent one with 63% frequency, while the S-8 allele was the least common allele with a rate of 4%. In allele combinations, the SI-So allele combination was the most common allele combination with a rate of 18%. and trilateral allele combinations (S-1-S-6-S-7 and S-1-S-6-S-8) were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.
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    Öğe
    Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR
    (Galenos Publ House, 2023) Karatas, Merve Dilek; Hazrati, Nahid; Yuksel Ozmen, Canan; Hasanzadeh, Mohammad; Altintas, Serdar; Akcay, Mehmet Emin; Ergul, Ali
    Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Huryemez, Sah elmasi, Tokat, Demir elmasi etc.) obtained from the National Collection of Ataturk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as 'Partially Incompatible' and 12 genotypes involving 4 S-alleles were assigned 'Totally Incompatible'. No S-alleles were observed in 2 genotypes (Pancarlik and Huryemez) which exhibited 'Compatibility' status.