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Öğe Analyzing the Some Biochemical Parameters of Diabetes Mellitus and Obese Patients who Applied to Siirt State Hospital Endocrine Polyclinic and Their Prevalence(Amer Inst Physics, 2016) Karagecili, Hasan; Yerlikaya, Emrah; Aydin, Ruken ZeynepObesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m(2). Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose p<0.003, age p<0.001. According to gender between women and men serum levels, ALT and AST levels; p<0.006 and cholesterol; p<0.04 were detected. According to participants education level blood biochemistry levels were observed statisticaly different p<0.001 with non-literacy group. In conclusion, obese and obese+diabetes patients blood serum values nearly close to each other. Obese subjects were been diabetic obese with age. In women obesity and diabetes mellitus prevalence were seen too much.Öğe Antioxidant, Antidiabetic, Anticholinergic, and Antiglaucoma Effects of Magnofluorine(Mdpi, 2022) Durmaz, Lokman; Kiziltas, Hatice; Guven, Leyla; Karagecili, Hasan; Alwasel, Saleh; Gulcin, IlhamiMagnofluorine, a secondary metabolite commonly found in various plants, has pharmacological potential; however, its antioxidant and enzyme inhibition effects have not been investigated. We investigated the antioxidant potential of Magnofluorine using bioanalytical assays with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)), N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD center dot+), and 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot) scavenging abilities and K-3[Fe(CN)(6)] and Cu2+ reduction abilities. Further, we compared the effects of Magnofluorine and butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), alpha-Tocopherol, and Trolox as positive antioxidant controls. According to the analysis results, Magnofluorine removed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals with an IC50 value of 10.58 mu g/mL. The IC50 values of BHA, BHT, Trolox, and alpha-Tocopherol were 10.10 mu g/mL, 25.95 mu g/mL, 7.059 mu g/mL, and 11.31 mu g/mL, respectively. Our results indicated that the DPPH center dot scavenging effect of Magnofluorine was similar to that of BHA, close to that of Trolox, and better than that of BHT and alpha-tocopherol. The inhibition effect of Magnofluorine was examined against enzymes, such as acetylcholinesterase (AChE), alpha-glycosidase, butyrylcholinesterase (BChE), and human carbonic anhydrase II (hCA II), which are linked to global disorders, such as diabetes, Alzheimer's disease (AD), and glaucoma. Magnofluorine inhibited these metabolic enzymes with Ki values of 10.251.94, 5.991.79, 25.411.10, and 30.563.36 nM, respectively. Thus, Magnofluorine, which has been proven to be an antioxidant, antidiabetic, and anticholinergic in our study, can treat glaucoma. In addition, molecular docking was performed to understand the interactions between Magnofluorine and target enzymes BChE (D: 6T9P), hCA II (A:3HS4), AChE (B:4EY7), and alpha-glycosidase (C:5NN8). The results suggest that Magnofluorine may be an important compound in the transition from natural sources to industrial applications, especially new drugs.Öğe Antioxidant, antidiabetic, antiglaucoma, and anticholinergic effects of Tayfi grape (Vitis vinifera): A phytochemical screening by LC-MS/MS analysis(De Gruyter Poland Sp Z O O, 2023) Karagecili, Hasan; Izol, Ebubekir; Kirecci, Ekrem; Gulcin, IlhamiGrapes (Vitis vinifera), grape extracts, and grape products are known to possess beneficial effects. Antioxidant activity and enzyme inhibition activities of Tayfi grape (Vitis vinifera) extracts were studied and compared to standards. The IC50 values of the ethanol extract of the Tayfi grape's scavenging abilities for ABTS(center dot+) and DPPH. radicals were found to be 5.9 and 16.1 mu g/mL, respectively, higher than those of positive controls. Also, the phenolic and flavonoid ingredients of the Tayfi grape seed ethanol extract were measured to be 82.8 mg GAE/g and 91 mg QE/g. The Tayfi grape seed water and ethanol extracts exhibited IC50 values of 5.3 and 5.8 mu g/mL toward a-glycosidase, respectively; 385.2 and 567.6 mu g/mL toward a-amylase; 27.1 and 13.8 mu g/mL toward acetylcholinesterase (AChE); and 54.7 and 12.6 mu g/mL toward CA II. Twenty-two biomolecules were detected by LC- MS/MS. The four types of conventional antibiotics utilized by hospitals proved ineffective against Staphylococcus aureus and Escherichia coli bacteria. The Tayfi grape ethanol and water extracts had high AChE, alpha-glycosidase, and CA II inhibitions in addition to having antioxidant activity. The use of Tayfi grape extracts for pharmacological purposes in individuals with the diseases mentioned above can be sustained by clinical pharmacology studies.Öğe Antioxidant, Antiglaucoma, Anticholinergic, and Antidiabetic Effects of Kiwifruit (Actinidia deliciosa) Oil: Metabolite Profile Analysis Using LC-HR/MS, GC/MS and GC-FID(Mdpi, 2023) Ozden, Eda Mehtap; Bingol, Zeynebe; Mutlu, Muzaffer; Karagecili, Hasan; Koksal, Ekrem; Goren, Ahmet C.; Alwasel, Saleh H.Determining the antioxidant abilities and enzyme inhibition profiles of medicinally important plants and their oils is of great importance for a healthy life and the treatment of some common global diseases. Kiwifruit (Actinidia deliciosa) oil was examined and researched using several bioanalytical methods comprehensively for the first time in this research to determine its antioxidant, antiglaucoma, antidiabetic and anti-Alzheimer's capabilities. Additionally, the kiwifruit oil inhibitory effects on acetylcholinesterase (AChE), carbonic anhydrase II (CA II), and alpha-amylase, which are linked to a number of metabolic illnesses, were established. Furthermore, LC-HRMS analysis was used to assess the phenolic content of kiwifruit oil. It came to light that kiwifruit oil contained 26 different phenolic compounds. According to the LC-HRMS findings, kiwifruit oil is abundant in apigenin (74.24 mg/L oil), epigallocatechin (12.89 mg/L oil), caryophyllene oxide (12.89 mg/L oil), and luteolin (5.49 mg/L oil). In addition, GC-MS and GC-FID studies were used to ascertain the quantity and chemical composition of the essential oils contained in kiwifruit oil. Squalene (53.04%), linoleoyl chloride (20.28%), linoleic acid (2.67%), and palmitic acid (1.54%) were the most abundant compounds in kiwifruit oil. For radical scavenging activities of kiwifruit oil, 1,1-diphenyl-2-picryl-hydrazil (DPPH center dot) and 2,2 '-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(center dot+)) radicals scavenging techniques were examined. These methods effectively demonstrated the potent radical scavenging properties of kiwifruit oil (IC50: 48.55 mu g/mL for DPPH center dot, and IC50: 77.00 mu g/mL for ABTS(center dot+) scavenging). Also, for reducing capabilities, iron (Fe3+), copper (Cu2+), and Fe3+-2,4,6-tri(2-pyridyl)-S-triazine (TPTZ) reducing abilities were studied. Moreover, kiwifruit oil showed a considerable inhibition effect towards hCA II (IC50: 505.83 mu g/mL), AChE (IC50: 12.80 mu g/mL), and alpha-amylase (IC50: 421.02 mu g/mL). The results revealed that the use of kiwifruit oil in a pharmaceutical procedure has very important effects due to its antioxidant, anti-Alzheimer, antidiabetic, and antiglaucoma effects.Öğe CARBONIC ANHYDRASE ENZYME FROM THE Alectoris chukar LIVER PURIFICATION CHARACTERIZATION AND ASSESSMENT OF ENZYME KINETICS AGAINST SOME PESTICIDES AND METAL TOXICITY(Parlar Scientific Publications (P S P), 2021) Karagecili, Hasan; Demirdag, Ramazan; Yerlikaya, EmrahCarbonic anhydrases (CAs) take major roles in many pathological situations associated with convulsions, carcinoma, glaucoma, weight problems, etc. In former researches; using tissues and erythrocytes of a wide range of organisms like; dogs, swine, sheep, chickens, bees, fish, goats, bovines, bacteria, and humans, CA enzymes have been purified and characterized. With this research, the CA enzyme has purified from Alectoris chukar liver tissue with 1.23 EU x mg(-1) of specific activity, 55.5% purification yield, and 460.7 purification folds. The purity of the purified enzyme has been approved by SDS-PAGE. The molecular weight was estimated to be about 28.4 kDa. As the characterization of CA enzyme's in the Alectoris chukar liver has been done; the optimum ionic strength-50 mM Tris-SO4. the optimum pH-8.0, and the optimum temperature-35 degrees C have been decided. K-M and V-max values were calculated by Lineweaver Burk graph. K-M constants were estimated as 1.035 mM, V-max, values as 0.02 molxmin(-1) for 4-nitrophenyl acetate. Inhibitory consequences of some pesticides (Spinosad and Dimethoate) and metal ions (Al3+, Cu2+, Ba2+, Fe2+, Mn2+, Se2+) have been analyzed on the purified CA enzyme. Inhibition graphics were drawn to find the IC50 values of metals and pesticides displaying inhibition.Öğe Carbonic Anyhdrase Enzyme From The Siirt Mohair Goat Liver :Purification, Characterization and Assessment of Enzyme Kinetics Against Metal Toxicity(2017) Yerlikaya, Emrah; Karagecili, Hasan; Demirdag, Ramazan; Kaya, Mustafa OguzhanBecause of their physiological and clinical roles, carbonic anhydrases (CAs) are the most studied enzymes. In earlier studies; CA enzymes have been purified and characterized from the tissues and erythrocytes of many organisms such as; dog, swine, sheep, chicken, bee, fish, bovine, bacteria and human. In this study, the CA enzyme has purified from Siirt Mohair Goat liver tissue with 1930.84 EU x mg-1 of specific activity, 57.28% of purification yield and 80.55 of purification folds. The purity of the purified enzyme has confirmed by SDS-PAGE. As the characterization of CA enzyme’s in Siirt Mohair Goat liver has been done; the optimum ionic strength=25 mM, the optimum pH= 8.0, the optimum temperature= 40ºC and the stable pH= 7.0 has been determined. Inhibitory effects of some metal ions have been examined on the purified CA enzyme. IC50 values of inhibiting metal ions were found as 2.24, 2.76, 2.36, 3.20, 2.55, 2.25, 3.28, 2.13, 3.10, 1.75, 2.16 and 3.50 mM for Al3+, Ni2+, Cd2+, Cu2+, Pb2+, Ba2+, Zn2+, B3+, Fe3+, Se2+, Ag+ and Co2+ respectively. As a result, CA enzyme was first purified from theÖğe Comprehensive Metabolite Profiling of Berdav Propolis Using LC-MS/MS: Determination of Antioxidant, Anticholinergic, Antiglaucoma, and Antidiabetic Effects(Mdpi, 2023) Karagecili, Hasan; Yilmaz, Mustafa Abdullah; Erturk, Adem; Kiziltas, Hatice; Guven, Leyla; Alwasel, Saleh H.; Gulcin, IlhamiPropolis is a complex natural compound that honeybees obtain from plants and contributes to hive safety. It is rich in phenolic and flavonoid compounds, which contain antioxidant, antimicrobial, and anticancer properties. In this study, the chemical composition and antioxidant activities of propolis were investigated; ABTS(center dot+), DPPH center dot and DMPD center dot+ were prepared using radical scavenging antioxidant methods. The phenolic and flavonoid contents of propolis were 53 mg of gallic acid equivalent (GAE)/g and 170.164 mg of quercetin equivalent (QE)/g, respectively. The ferric ion (Fe3+) reduction, CUPRAC and FRAP reduction capacities were also studied. The antioxidant and reducing capacities of propolis were compared with those of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol and Trolox reference standards. The half maximal inhibition concentration (IC50) values of propolis for ABTS(center dot+), DPPH center dot and DMPD center dot+ scavenging activities were found to be 8.15, 20.55 and 86.64 mu g/mL, respectively. Propolis extract demonstrated IC50 values of 3.7, 3.4 and 19.6 mu g/mL against alpha-glycosidase, acetylcholinesterase (AChE) and carbonic anhydrase II (hCA II) enzyme, respectively. These enzymes' inhibition was associated with diabetes, Alzheimer's disease (AD) and glaucoma. The reducing power, antioxidant activity and enzyme inhibition capacity of propolis extract were comparable to those demonstrated by the standards. Twenty-eight phenolic compounds, including acacetin, caffeic acid, p-coumaric acid, naringenin, chrysin, quinic acid, quercetin, and ferulic acid, were determined by LC-MS/MS to be major organic compounds in propolis. The polyphenolic antioxidant-rich content of the ethanol extract of propolis appears to be a natural product that can be used in the treatment of diabetes, AD, glaucoma, epilepsy, and cancerous diseases.Öğe Comprehensively Revealing the Profile of Pistacia vera L. cv. Siirt Turpentine - Antioxidant, Antidiabetic, Anti-Alzheimer, and Antiglaucoma Effects(Acg Publications, 2023) Karagecili, Hasan; Yilmaz, Mustafa Abdullah; Alwasel, Saleh H.; Arik, Mustafa; Gulcin, IlhamiPistacia species containing turpentine which has protective effects and used as a medicine against diabetes, and infections for a long time. To interpret the antioxidant capacities of turpentine from Pistacia vera L. cv. Siirt, 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS(center dot+)) and 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH center dot), Fe3+- 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) and Cu2+ reducing ability were studied and compared to reference antioxidants of BHT, BHA, alpha-Tocopherol, and Trolox. The IC50 of turpentine for ABTS(center dot+) and DPPH center dot scavenging capacities were determined to be lower than reference antioxidant standards as 231.1 mu g/mL and 346.6 mu g/mL, consequently. The total flavonoid and phenolic contents in turpentine were measured as 12.5 mg GAE/g and 38.852 mg QE/g, respectively. For determination of antidiabetic, anti-Alzheimer, and antiglaucoma effects of turpentine, acetylcholinesterase (AChE), alpha-glycosidase and carbonic anhydrase II (hCA II) enzymes inhibition abilities of turpentine were performed. The IC50 values of turpentine extract against alpha-glycosidase, hCA II, and AChE enzymes were found as 2.04, 3.59 and 14.6 mu g/mL, respectively. The quantity of protocatechuic acid and naringenin were determined using LC-MS/MS against 53 standards. The results clearly demonstrated polyphenolics in P. vera L. cv. Siirt turpentine is a natural product has potential usage in amelioration of glaucoma, AD, diabetes, and epilepsy.Öğe Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)-A Chemical Profiling by LC-MS/MS(Mdpi, 2023) Karagecili, Hasan; Izol, Ebubekir; Kirecci, Ekrem; Gulcin, IlhamiZivzik pomegranate (Punica granatum) has recently sparked considerable interest due to its nutritional and antioxidant properties. To evaluate the antioxidant capacities of P. granatum juice, ethanol (EEZP), and water (WEZP) extracts from peel and seed, the antioxidant methods of 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS(center dot)+) scavenging, 1,1-diphenyl-2picrylhydrazyl free radical (DPPH center dot) scavenging, Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing, Fe3+ reducing, and Cu2+ reducing methods were used. The antioxidant capacities of samples were compared with the most commonly used synthetic antioxidants, i.e., BHA, BHT, ff-tocopherol, and Trolox. In terms of setting an example, the IC50 values of EEZP for ABTS(center dot)+ and DPPH center dot scavenging activities were found to be lower than standards, at 5.9 and 16.1 mu g/mL, respectively. The phenolic and flavonoid contents in EEZP peel were 59.7 mg GAE/g and 88.0 mg QE/g, respectively. Inhibition of ff-glycosidase, ff-amylase, acetylcholinesterase, and human carbonic anhydrase II (hCA II) enzymes was also investigated. EEZP demonstrated IC50 values of 7.3 mu g/mL against ff-glycosidase, 317.7 mu g/mL against ff-amylase, 19.7 mu g/mL against acetylcholinesterase (AChE), and 106.3 mu g/mL against CA II enzymes. A total of 53 phenolic compounds were scanned, and 30 compounds were determined using LC-MS/MS. E. coli and S. aureus bacteria were resistant to all four antibiotics used as standards in hospitals.Öğe Evaluation of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and ?-Glycosidase Inhibition Effects and Antioxidant Activity of Baicalin Hydrate(Mdpi, 2023) Durmaz, Lokman; Karagecili, Hasan; Gulcin, IlhamiBaicalin is the foremost prevalent flavonoid found in Scutellaria baicalensis. It also frequently occurs in many multi-herbal preparations utilized in Eastern countries. The current research has assessed and compared the antioxidant, antidiabetic, anticholinergic, and antiglaucoma properties of baicalin hydrate. Baicalin hydrate was tested for its antioxidant capacity using a variety of techniques, including N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD center dot+) scavenging activity, 2,2 '-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS(center dot+)) scavenging activity, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot) scavenging activity, potassium ferric cyanide reduction ability, and cupric ions (Cu2+) reducing activities. Also, for comparative purposes, reference antioxidants, such as butylated hydroxyanisole (BHA), Trolox, alpha-Tocopherol, and butylated hydroxytoluene (BHT) were employed. Baicalin hydrate had an IC50 value of 13.40 mu g/mL (r(2): 0.9940) for DPPH radical scavenging, whereas BHA, BHT, Trolox, and alpha-Tocopherol had IC50 values of 10.10, 25.95, 7.059, and 11.31 mu g/mL for DPPH center dot scavenging, respectively. These findings showed that baicalin hydrate had comparably close and similar DPPH center dot scavenging capability to BHA, alpha-tocopherol, and Trolox, but it performed better than BHT. Additionally, apart from these studies, baicalin hydrate was tested for its ability to inhibit a number of metabolic enzymes, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II), and alpha-glycosidase, which have been linked to several serious illnesses, such as Alzheimer's disease (AD), glaucoma, and diabetes, where the K-i values of baicalin hydrate toward the aforementioned enzymes were 10.01 +/- 2.86, 3.50 +/- 0.68, 19.25 +/- 1.79, and 26.98 +/- 9.91 nM, respectively.Öğe Evaluation of Infection Factor and Antibiotic Resistance Distributions in Palliative Care Patients Developed Urinary Tract Infection(Gazi Univ, Fac Med, 2023) Yildirim, Filiz; Ozturk, Reyhan; Kaynar, Pinar Mursaloglu; Yildirim, Zuhal; Karagecili, HasanObjectives: In this study, it was aimed to identify the causative bacteria of urinary tract infections, to determine antibiotic susceptibility, and to examine acute phase markers in patients hospitalized in the palliative care service of Ankara Polatli Duatepe State Hospital between January 2019 and December 2020. Materials and Methods: These sex, age and, detected diseases of a total of 72 palliative care patients included in our study were analyzed retrospectively. To determine the causative agents of urinary tract infections in these patients, Gram staining was performed on the bacterial cultures that developed in the urine samples, and the Vitek-2 (bioMerieux, France) automatic test device was used to identify these cultures and determine their antibiotic susceptibility. In the blood sample staken from these patients, leukocyte (WBC), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) measurements were also made. Results: The causative agents of urinary tract infections of these patients are Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumannii, Citrobacter koseri, Morganella morganii ssp. morganii, Enterobacter cloacae complex, and Gram-positive bacteria Enterococcus faecium, Enterococcus spp., and Staphylococcus epidermidis were found. Various antibiotics were determined to have different levels of sensitivity. Conclusions: It is important to determine the causative agents of urinary tract infections, which are among the most frequently detected infections in palliative care patients, and to determine antibiotic susceptibility and acute phase response markers fo these agents. Mortality and morbidity will be reduced by determining the causative agents of these infections and applying appropriate antibiotic treatments.Öğe Hamamelitannin's Antioxidant Effect and Its Inhibition Capability on ?-Glycosidase, Carbonic Anhydrase, Acetylcholinesterase, and Butyrylcholinesterase Enzymes(Mdpi, 2024) Durmaz, Lokman; Karagecili, Hasan; Erturk, Adem; Ozden, Eda Mehtap; Taslimi, Parham; Alwasel, Saleh; Gulcin, IlhamiHamamelitannin (2 ',5-di-O-galloyl-hamamelose) bears two-gallate moieties in its structure, and is a natural phenolic product in the leaves and the bark of Hamamelis virginiana. The antioxidant capacity of hamamelitannin was evaluated by a range of methods, with the following findings: the ability to reduce potassium ferric cyanide; the scavenging of N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD center dot+); the scavenging of 2,2 '-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS center dot+); the scavenging of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot); and the ability to reduce cupric ions (Cu2+). Additionally, reference antioxidants of alpha-Tocopherol, butylated hydroxyanisole (BHA), Trolox, and butylated hydroxytoluene (BHT) were used for comparison. For DPPH radical scavenging, hamamelitannin had an IC50 value of 19.31 mu g/mL, while the IC50 values for BHA, BHT, Trolox, and alpha-Tocopherol were 10.10, 25.95, 7.05, and 11.31 mu g/mL, respectively. The study found that hamamelitannin functioned similarly to BHA, alpha-tocopherol, and Trolox in terms of DPPH center dot scavenging, but better than BHT. Additionally, as a polyphenolic secondary metabolite, the hamamelitannin inhibition capability of several metabolic enzymes was demonstrated, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase I (CA I), carbonic anhydrase II (CA II) and alpha-glycosidase. The Ki values of hamamelitannin exhibited 7.40, 1.99, 10.18, 18.26, and 25.79 nM toward AChE, BChE, hCA I, hCA II, and alpha-glycosidase, respectively.Öğe Potential antioxidant, anticholinergic, antidiabetic and antiglaucoma activities and molecular docking of spiraeoside as a secondary metabolite of onion (Allium cepa)(Elsevier, 2023) Durmaz, Lokman; Kiziltas, Hatice; Karagecili, Hasan; Alwasel, Saleh; Gulcin, LhamiOnion contains many dietary and bioactive components including phenolics and flavonoids. Spiraeoside (quercetin-4-O-b-D-glucoside) is one of the most putative flavonoids in onion. Several antioxidant techniques were used in this investigation to assess the antioxidant capabilities of spiraeoside, including 1,1diphenyl-2-picrylhydrazyl radical (DPPHGreek ano teleia) scavenging, N,N-dimethyl-p-phenylenediamine radical (DMPD'') scavenging, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS'') scavenging activities, cupric ions (Cu2') reducing and potassium ferric cyanide reduction abilities. In contrast, the water-soluble a-tocopherol analogue trolox and the conventional antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and a-tocopherol were utilized as the standards for evaluation. Spiraeoside scavenged the DPPH radicals an IC50 of 28.51 mu g/mL (r2: 0.9705) meanwhile BHA, BHT, trolox, and a-tocopherol displayed IC50 of 10.10 mu g/mL (r2: 0.9015), 25.95 mu g/mL (r2: 0.9221), 7.059 mu g/mL (r2: 0.9614) and 11.31 mu g/mL (r2: 0.9642), accordingly. The results exhibited that spiraeoside had effects similar to BHT, but less potent than a-tocopherol, trolox and BHA. Also, inhibitory effects of spiraeoside were evaluated toward some metabolic enzymes including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II) and a-glycosidase, which are related to a number of illnesses, such as Alzheimer's disease (AD), diabetes mellitus and glaucoma disorder. Spiraeoside exhibited IC50 values of 4.44 nM (r2: 0.9610), 7.88 nM (r2: 0.9784), 19.42 nM (r2: 0.9673) and 29.17 mM (r2: 0.9209), respectively against these enzymes. Enzyme inhibition abilities were compared to clinical used inhibitors including acetazolamide (for CA II), tacrine (for AChE and BChE) and acarbose (for a-glycosidase). Spiraeoside demonstrated effective antioxidant, anticholinergic, antidiabetic and antiglaucoma activities. With these properties, it has shown that Spiraeoside has the potential to be a medicine for some metabolic diseases. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Öğe Siirt Tiftik Keçisi Böbrek Dokusundan Karbonik Anhidraz Enziminin Saflaştırılması, Karakterizasyonu ve Bazı Biyokimyasal Özelliklerinin Araştırılması(2016) Yerlikaya, Emrah; Karagecili, Hasan; Demirdag, Ramazan; Kaya, Mustafa OguzhanCarbonic anhydrase (CA) (E.C:4.2.1.1) is a group of metalloenzyme containing zinc widely distributed from the simplest organisms such as bacteria to the most complex organisms such as plants and animals. CA inhibitors are some of the principal drugs used in the management of canine and feline glaucoma. In earlier studies; CA enzymes successfully have been purified and characterized from many living things such as; sheep, chicken, fish, bovine and human. In this study, the CA enzyme has purified from Siirt Mohair Goat kidney tissue with 902.9 EU x mg-1 of specific activity, 50.19% of purification yield and 83.54 of purification folds by using Sepharose-4B-L-tyrosine-sulfonamide affinity column. The purity of the purified enzyme has confirmed by SDS-PAGE. As the characterization of CA enzyme’s in Siirt Mohair Goat kidney has been done; the optimum ionic strength=25mM, the optimum pH=8.5, the optimum temperature=45ºC and the stable pH=7.0 has been determined. Inhibitory effects of Al3+, Ni2+, Cd2+, Pb2+, Ba2+, Zn2+, B3+, Fe3+, Se2+, Ag+ and Co2+ metal ions have been examined on the purified CA enzyme. Inhibition graphics have been drawn in order to find the IC50 values of metals showing inhibition.Öğe The Investigation of the Some Body Parameters of Obese and (Obese plus Diabetes) Patients with Using Bioelectrical Impedance Analysis Techniques(Amer Inst Physics, 2016) Yerlikaya, Emrah; Karagecili, Hasan; Aydin, Ruken ZeynepObesity is a key risk for the development of hyperglycemia, hypertension, hyperlipidemia, insulin resistance and is totally referred to as the metabolic disorders. Diabetes mellitus, a metabolic disorder, is related with hyperglycemia, altered metabolism of lipids, carbohydrates and proteins. The minimum defining characteristic feature to identify diabetes mellitus is chronic and substantiated elevation of circulating glucose concentration. In this study, it is aimed to determine the body composition analyze of obese and (obese+diabetes) patients. We studied the datas taken from three independent groups with the body composition analyzer instrument. The body composition analyzer calculates body parameters, such as body fat ratio, body fat mass, fat free mass, estimated muscle mass, and base metabolic rate on the basis of data obtained by Dual Energy X-ray Absorptiometry using Bioelectrical Impedance Analysis. All patients and healthy subjects applied to Siirt University Medico and their datas were taken. The Statistical Package for Social Sciences version 21 was used for descriptive data analysis. When we compared and analyzed three groups datas, we found statistically significant difference between obese, (obese+diabetes) and control groups values. Anova test and tukey test are used to analyze the difference between groups and to do multiple comparisons. T test is also used to analyze the difference between genders. We observed the statistically significant difference in age and mineral amount p<0.00 between (diabetes+obese) and obese groups. Besides, when these patient groups and control group were analyzed, there were significant difference between most parameters. In terms of education level among the illiterate and university graduates; fat mass kg, fat percentage, internal lubrication, body mass index, water percentage, protein mass percentage, mineral percentage p<0.05, significant statistically difference were observed. This difference especially may result of a sedentary lifestyle.
