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Öğe Comparative Adaptation Responses of Melon (Cucumis melo L.) Genotypes to Salinity Stress(Tarbiat Modares Univ, 2021) Erdinc, C.; Inal, B.; Erez, E.; Ekincialp, A.; Sensoy, S.The objective of this work was to understand the mechanisms of physiological, biochemical, and molecular responses to salinity stress of three Turkish melon genotypes (YYU 1, YYU 4 and CU 196) and cv. Ananas. The study used Randomized Complete Block Design (RCBD) and pots were irrigated with Hoagland nutrient solution after two-leaf stage until harvesting by 50 and 75 mM NaCl concentrations. For evaluation of responses, chlorophyll and carotenoid content, total phenolic and flavonoid amount, proline variations, and nutrient elements were determined. Moreover, qRT-PCR analyses were performed to identify the expression level of six TF (Transcription Factors) genes (WRKY24, TCP15, CmHD-Zip, mTERF2, Dof3 and CmADH2). Increase in salt application led to increase in chlorophyll content in the melon genotypes, but decrease (about 55%) in cv. Ananas. Phenolic, flavonoid, and proline contents varied based on the melon genotypes, but generally increased in Ananas. Expression levels of TCP15 and WRKY24 showed more fold change at 75 mM NaCl treatment. On the other hand, the expression of CmADH2 and Dof3 showed more fold change at 50 mM NaCl treatment. Finally, according to adaptation mechanisms of melon genotypes, the study might help in selection and detection of the salt tolerant ones.Öğe EXPRESSION OF NITROGEN TRANSPORTER GENES IN WHEAT LEAVES ( Triticum aestivum L.) AND FIELD PERFORMANCES UNDER ORGANIC CONDITIONS(Pakistan Agricultural Scientists Forum, 2024) Bayhan, M.; Ozkan, R.; Yildirim, M.; Inal, B.This study aimed to investigate the expression patterns of nitrogen transporter genes in wheat (Triticum aestivum L.) grown in organic farming systems. Expression profiling of these genes will provide valuable insights into the molecular mechanisms involved in nitrogen uptake and transport in wheat plants. In addition, the agronomic responses of the wheat genotypes were evaluated under organic conditions. This will help in the creation of genotypes that support effective nitrogen use in organic farming systems. The study showed that the nitrate transporter gene family member TaNRT2.1 exhibited the highest expression level. The Alada cultivar performed well in terms of yield under field conditions and showed the highest expression levels of TaNRT2.1, NRT2.1, and TaGS1. The 6DZT-03-01 genotype had the highest expression levels of NPF6.3 and AMT2.1. Alada, YE-4, and YE-31 were identified as the most suitable genotypes for organic farming. Several genes, including TaNRT2.1, were more highly expressed in the high-yielding genotype, Alada, than in the other genotypes. This suggested that nitrate and ammonium transporters play an important role in determining how different genotypes of wheat take in nitrogen. Further research is needed to understand the complex mechanisms that contribute to genotypic variation in nitrogen uptake in wheat. To fully understand the genetic basis of nitrogen uptake in wheat and to develop ways to improve nutrient management in organic farming systems, the expression levels of other genes involved in nitrogen metabolism should also be examined.Öğe GENE EXPRESSION ANALYSES OF SOME TRANSPOSONS, PHYSIOLOGICAL AND MORPHOLOGICAL CHANGES IN MELON CULTIVARS EXPOSED TO DROUGHT STRESS(Pakistan Agricultural Scientists Forum, 2022) Cicek, S.; Altintas, S.; Inal, B.As sessile organisms, plants are exposed to a wide variety of abiotic and biotic stress. In this case, plants put very complex molecular mechanisms into action against these adverse environmental conditions. Especially at the gene level, it alarms many genes (transcription factors, promoters, miRNA, lncRNA, structural genes, transposons etc.). Transposons, which are among these genes, have recently been studied for their functions. However, it is not known exactly how the plants show the transposons activity, especially in abiotic stress situations. In this respect, expression situations of diverse transposons in plants are still very curious. In this study, various transposon genes belonging to different plant species (tobacco-specific Tto-1, Tto-5 and Tnt-1, Excoecaria agallocha-specific EARE1, rice-specific Tos17, barley-specific BARE1, melon-specific Reme1, Triticum-specific Ttd1a) were used to measure horizontal transfer activities and the expression levels of these transposons were measured by q-RT PCR in sensitive and resistant melon accessions treated with drought stress. Additionally, chlorophyll content, glutathione reductase (GR) activity and some morphometric data were measured in these melon accessions under drought stress. Sensitive and resistant accessions showed significant differences in terms of both gene expression and physiological parameters, indicating the possible role of transposons in drought conditions.Öğe In silico analysis of Phaseolus vulgaris L. metalloprotease FtsH gene: characterization and expression in drought and salt stress(Springer, 2024) Inal, B.; Muslu, S.; Yigider, E.; Kasapoglu, A. G.; Ilhan, E.; Ciltas, A.; Yildirim, E.Proteases help plants maintain protein quality and break down protein subsets in reply to environmental, developmental, biotic, and abiotic stressors. Filamentation temperature-sensitive H (FtsH) is an ATP-dependent metalloprotease detected in both prokaryotes and eukaryotes cells. The present research, the FtsH gene in the bean, which has an important place in the legume family and is an important agricultural product, was characterized for the first time using various bioinformatic tools, and qRT-PCR measured its expression level. In the study, effectively identified and characterized 17 FtsH genes present in genome the of Phaseolus vulgaris. The MW of FtsH proteins varied from 71.16 to 147.07 kDa, their amino acid lengths ranged from 642 to 1284, and their pI values varied from 5.39 to 9.60. Interestingly, the distribution of these 17 distinct PvFtsH genes across the 8 chromosomes was not uniform, exhibiting an uneven pattern throughout the genome. A pair of segmental duplication fragments were found, revealing probable processes of gene expansion and evolution. Collinearity with related genes in Arabidopsis and rice was thoroughly examined to determine the evolutionary conservation and differentiation of PvFtsH genes. Additionally, we used RNAseq and qRT-PCR to investigate the expression patterns of PvFtsH in leaf tissue under salt and drought conditions. Our data showed unique expression patterns, suggesting PvFtsH may respond to environmental and physiological stressors. Overall, this work makes major contributions to our understanding of PvFtsH genes and their roles in the context of gene evolution, chromosomal distribution, and expression patterns under various environmental situations.Öğe THE COMPARISON OF TOTAL PHENOLIC CONTENT, ANTIOXIDANT CAPACITY AND MOLECULAR ANALYSIS OF SOME SELECTED TURKISH APPLE (MALUS SPP.) GENOTYPES(Aloki Applied Ecological Research And Forensic Inst Ltd, 2017) Inal, B.; Ozrenk, K.; Erez, M. E.; Altintas, S.In the present study, eight apple genotypes were analyzed using internal cpDNA trnL intron sequence. Furthermore, these genotypes species were evaluated for their antioxidant activity, total phenolic and flavonoid contents. Genotypes in question show quite a variation in biochemical characteristics and molecular systematic analysis. One of the genotypes Helesan was the best graded genotype based on the high content of the total phenolic (12,21 +/- 1,25) and total flavonoid (7,98 +/- 0,26) content. As a result of the molecular analysis, which is one of the main aims of the study, genotypes are comprised of three main clades with robust bootstrap (95) value. It was concluded that these genotypes could be useful to improve genetic diversity among genotypes for breeding.
