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Öğe Identification of hub genes and key pathways targeted by miRNAs in pancreatic ductal adenocarcinoma: MAPK3/8/9 and TGFBR1/2(Elsevier, 2024) Gungormez, CigdemDuctal adenocarcinoma of the pancreas(PDAC) is one of the malignancies with the worst prognosis still among solid tumors. Local and distant spread is observed at the time of diagnosis in a very significant part of patients. In recent years, significant improvements have been made in pancreatic cancer surgery, and serious decreases in morbidity and mortality rates have been detected, especially in specific centers. Studies on target gene therapy in all types of cancer, including pancreatic cancer, are very laborious and costly. For this reason, it provides convenience for research aimed at determining clinical markers by using bioinformatics software. In this study, it was aimed to determine the target gene and pathway by performing transcriptome analysis of 14 control and 28 PDAC data belonging to GSE123377 and GSE 163031. As a result of the analysis, it was found that 49 genes were in direct interaction with PDAC by determining the targets of 46 miRNAs with DIANA Path, whose expression differences were determined as a result of the analysis. Based on the PPI topological analysis, it was determined that 46 miRNAs of prostate cancer directly target 12 hub proteins (BRAF, STAT3,TGFBR1,SMAD2,SMAD3, TP53, TGFBR2, KRAS, MAPK1,MAPK3, MAPK8 and MAPK9). Functional Enrichment Analysis and biological process; cell communication with 59.18% and signal transmission with 73.47%; It was observed that protein serine/ threonine kinase activity in molecular function was associated with a 22.45% effect on pathways. Thus, it is planned to support research by providing a system-level view by processing data networks for potential diagnostic biomarkers and target gene therapy for early diagnosis of PDAC.Öğe Immune plasma therapy in COVID-19 infection: the experience of Siirt(2021) Özüdoğru, Osman; Gungormez, Cigdem; Üyüklü, MehmetObjective: COVID-19 pandemic which is rapidly spreading in the world affects people in Turkey significantly. Various drug-methods for treatment are being tried and one of these applications is immune plasma transfusion. Materials and Methods: In this study, 400cc immune plasma treatment was evaluated retrospectively to show the effect of treatment applied to 23 patients with COVID-19 diagnosis in Siirt Training and Research Hospital. Results: It was observed that the use of mechanical ventilation was 47.82% before the immune plasma treatment, while this need decreased to 30.43% after 3 days. Nine out of 23 patients who received immune plasma therapy recovered by completing their treatment. Conclusion: Although the immune plasma applied on patients contributes to the recovery of patients, the evidence is yet insufficient. Therefore, more COVID-19 patients need to be observed to document the efficiency of immune plasma transfusion therapy.Öğe miRNA Profile and Bioinformatic Analysis for Diagnosis in Patients with Stage IIIA Colon Cancer(Springer/Plenum Publishers, 2022) Gungormez, Cigdem; Teker, Emine; Atmanoglu, Sema; Borazan, ErsinEarly diagnosis is a critical factor in deciding the outcome of colon cancer, as is the case with other types of cancers. Recent scientific developments have enabled the use of biomarkers for diagnosis and for designing treatment strategies for various cancer types. Further, identification of potential targets of these biomarkers will facilitate a better understanding of molecular processes. The aim of this study is to analyze microRNA expression profile, and through bioinformatic analyses determine the cellular processes of potential target genes and understand their molecular mechanism in stage IIIA colon cancer patients. The microRNA expression profiles of both normal and tumor tissues of seven patients were analyzed using the Affymetrix microarray system. The target genes were identified by performing a KEGG pathway analysis on eight miRNAs (hsa-miR-362-3p, hsa-miR-34c-5p, hsa-miR-34c-3p, hsa-miR-34a-3p, hsa-miR-19b-1-3p, hsa-miR-371a-5p, hsa-miR-941 ad hsa-miR-7-5p), which were selected through an array scan by using DIANA-miRPath v.3 bioinformatic analysis tool. Biological pathway and cellular component analyses were performed on 30 genes targeted by miRNAs using FunRich Gene Enrichment tool. These analyses indicated that the genes targeted by these eight miRNAs played a role in either cell communication (53%), signal transduction (60%) or apoptosis (20%) in stage IIIA colon cancer. Taken together, these data suggest that these miRNAs can be used as biomarkers in Stage IIIA colon cancer.Öğe miRNA Profile and Bioinformatic Analysis for Diagnosis in Patients with Stage IIIA Colon Cancer (Jun, 10.1007/s10528-021-10096-1, 2021)(Springer/Plenum Publishers, 2022) Gungormez, Cigdem; Teker, Emine; Atmanoglu, Sema; Borazan, Ersin[Abstract Not Available]
