Yazar "Gülendağ, Erman" seçeneğine göre listele

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    Chemical Composition and Physical Properties of Milk in Norduz Sheep
    (2023) Koca, Davut; Turgut, Ali Osman; Çetin, Nebi; Üner, Sefa; Gülendağ, Erman; Karagülle, Bahtiyar
    The objective of this study was to uncover the milk composition traits of Norduz sheep, which are bred in the Gürpınar district of the Van province, during the mid-term lactation period. The chosen ewes exhibited no signs of clinical or subclinical mastitis. A total of 104 sheep milk samples were meticulously collected to undergo comprehensive milk composition analysis. The chemical and physical attributes of Norduz sheep milk were methodically determined utilizing a milk autoanalyzer. The mean values for various milk constituents were as follows: milk fat (%), 2.48±0.11; solids-not-fat (SNF) (%), 10.76±0.08; milk protein (%), 5.09±0.04; lactose (%), 4.79±0.04; pH, 6.93±0.02; conductivity (mS/cm), 4.41±0.04; freezing point (°C), -0.602±0.80; salt (%), 0.8035±0.66; and density (kg/m3), 1039.73±0.39. Noteworthy negative and statistically significant correlations were observed between milk fat and protein (r=-0.36, p<0.001), milk fat and SNF (r=-0.32, p<0.001), as well as milk fat and lactose (r=-0.36, p<0.001). Conversely, positive and significant correlations emerged between SNF and milk protein (r=0.90, p<0.001), SNF and salt (r=0.87, p<0.001), and SNF and lactose (r=0.90, p<0.001). In summation, the protein content of Norduz sheep's milk exceeded that of the majority of sheep breeds reared in Türkiye. These findings carry the potential to make a valuable contribution to enhancing the milk composition of Norduz sheep.
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    Comparison of Antimicrobial Activities of Ethanol- and Water-Based Propolis Extracts on Various Foodborne Pathogens by Agar-Well Diffusion Method
    (2023) Acar, Bahar Onaran; Gülendağ, Erman
    Although ethanolic-based propolis extracts have been shown to have various biological activities, studies on the differences in the antimicrobial effects of water- and ethanol-based extracts, are lacking. Propolis preparations are now also sold as water-based products due to a number of factors, such as being safer for babies, being biocompatible, and some religious prohibitions on alcohol consumption. This study aimed to compare the antimicrobial effects of commercially available propolis preparations in the form of ethanol- and water-based extracts against various pathogenic microorganisms that cause foodborne infections. A total of 36 Gram-positive and Gram-negative bacterial strains from 8 different species were used in the study. As a result of the statistical analysis, only ethanol-based propolis from Turkey was effective against enterococci, Staphylococcus aureus, Bacillus cereus, Bacillus subtilis subsp. spizizenii, Salmonella spp., Listeria monocytogenes, Acinetobacter baumannii, and Pseudomonas aeroginosa, except Escherichia coli. It was concluded that the water-based extract did not have a significant inhibitory effect on the bacteria tested. It was determined that both ethanol- and water-based propolis tested in the study showed the most inhibitory effect on Acinetobacter baumannii. In addition, we can also conclude that ethanol-based propolis extract is more effective against Gram-positive bacteria than Gram-negative bacteria.
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    Evaluation of CBC, Lipid Profile, Oxidative Stress Biomarkers, Total Thiol, Native Thiol, and Disulfide Levels in Dogs Diagnosed with Parvoviral Enteritis Without Clinical Findings
    (2022) Kurtdede, Efe; Börkü, Mehmet Kazım; Gür, Berna; Doğan, Yalçın; Sevim, Kadir; Gülendağ, Erman
    In this study, whole blood cell count, metabolic and inflammatory biomarkers, and oxidative stress parameters were evaluated in dogs diagnosed with canine parvoviral enteritis by stool antigen testing performed 24-48 hours before any clinical findings arose. This study aimed to conduct effective evaluations of the status of the disease, the selection of treatment methods, and the prognosis of the disease. Thirty dogs with canine parvoviral enteritis positivity according to stool antigen test results 24-48 hours before the appearance of any clinical signs associated with parvo- viral enteritis (lethargy, vomiting, diarrhea, dehydration) were evaluated together with 10 healthy dogs. In analyses of the dogs with canine parvoviral enteritis, significant decreases were found for white blood cell (P < .001), neutrophil (P < .001), and monocyte (P = .011) counts and significant increases were found for alanine aminotransferase and aspartate aminotransferase (P < .001 for both). Significant increases (P < .001) were also determined for high-density lipoprotein, choles- terol, and triglyceride levels among the studied lipid metabolites and C-reactive protein, malondi- aldehyde, and paraoxonase-1 among inflammatory biomarkers. Significant decreases were found for total thiol (P = .002) and native thiol (P < .001), with which antioxidant levels were evaluated, and a significant increase was found for disulfide (P < .001), with which oxidation was evaluated. It was concluded that early changes in blood samples taken 24-48 hours before the onset of clinical symptoms in dogs with CPV, as reflected by values of CBC and metabolic and inflammatory bio- markers, may provide information about the severity of the disease, the choice of medical treat- ments to be applied, and prognosis.
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    Milk Composition Traits of Hamdani Crossbreed Sheep Raised Under Extensive Management
    (2023) Turgut, Ali Osman; Gülendağ, Erman; Koca, Davut; Üner, Sefa
    This study aimed to reveal the milk composition traits of Hamdani crossbred sheep (Hcs) raised under extensive conditions. A total of 96 sheep milk samples were collected during the early lactation stage. Selected ewes did not have clinical mastitis or subclinical mastitis. Milk composition analyses were performed using milk autoanalyzer. Mean values for milk fat (%), solid non-fat (SNF) (%), protein (%), lactose (%), salt (%) and pH, conductivity (mS/cm), freezing point (?C) and density (kg/m3) in Hcs were determined as 7.49±0.15, 8.69±0.08, 4.13±0.04, 3.89±0.04, 0.62±0.01, 6.93±0.04, 4.05±0.07, -0.5±0.01, and 1.027.84±0.38 respectively. In correlation analysis, negative and significant correlations were detected between milk fat and protein (r=- 0.368, p<0.001), milk fat and SNF (r=-0375, p<0.001) milk fat and lactose (r=-0.380, p<0.001). On the other hand, positive and significant correlations were detected between SNF and milk protein (r=0.999, p<0.001), SNF and salt (r=0.963, p<0.001), and SNF and lactose (r=0.976, p<0.001). As a result, the milk fat percentage of Hcs was higher compared to other native sheep breeds of Turkey. In addition, phenotypic variation for milk fat percentage was found to be high among individuals in the same flock. These findings may contribute to new studies to improve milk composition traits in Hamdani crossbred sheep.
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    PAEP gene restriction fragment length polymorphism and its effects on milk composition in cross-bred Hamdani sheep
    (2024) Turgut, Ali Osman; Gülendağ, Erman; Koca, Davut; Üner, Sefa
    ?-lactoglobulin (?LG) stands as the primary whey protein in ruminant milk, synthesized by mammary gland cells during lactation and encoded by the ?LG gene. This study aimed to assess the impact of ?LG gene exon II polymorphism on milk composition traits in crossbreed Hamdani sheep. Sheep were examined for clinical diseases and mastitis. Milk and blood samples were only collected from healthy ewes. The composition and physical properties of milk were analyzed using milk autoanalyzer. The ?LG gene exon II region's 452 bp PCR products were subjected to RFLP analysis using the RsaI restriction enzyme. Two genotypes, AA and AB, were identified for the ?LG gene exon II region, with A and B allele frequencies of 0.7 and 0.3, respectively. Statistical analysis, conducted with Minitab® (Version: 19.2020.2.0), revealed that the AA genotype is associated with a higher milk fat percentage p<0.05). However, no significant genotype effect was observed for other milk composition traits in cross-bred Hamdani sheep. These results suggest that PAEP genotypes could serve as valuable indicators for enhancing milk composition in cross-bred Hamdani sheep through breeding programs.
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    Relationships between the Postpartum Body Condition Score, Colostral IgG Content, Liver Lipid Metabolism and Oxidative Stress in Dairy Cows
    (2022) Kurtdede, Efe; Kurtdede, Arif; Kara, Erdal; Er, Mehmet Ba; Gülendağ, Erman
    His study aimed to evaluate the relationships between the postpartum body condition score (BCS)\rand colostral IgG, lipid metabolism, liver functions and oxidative stress parameters in dairy cows\rwith BCSs of ?3.5 and >3.5.\rThis investigation included 60 dairy cows of the same breed, that were 3-5 years old and had given\rtwo or more births in a dairy farm. Care was taken to ensure that the BCS changes of the cows\rwere <0.5 from the dry period to calving.\rIn the 30 cows with a BCS of ?3.5 within 24 hours of birth, the mean BCS level was 3.24±0.24 and\rthe mean serum ?-hydroxybutyric acid (BHBA) level was 0.36±0.07. In the 30 cows with a BCS of\r>3.5 within 24 hours of birth, the mean BCS level was 3.96±0.14 and the mean serum BHBA level\rwas 0.38±0.07.\rCompared to the values determined in cows with a BCS >3.5, serum non-esterified fatty acids\r(NEFA), high-density lipoprotein (HDL), paraoxonase-1 (PON-1) and malondialdehyde (MDA)\rlevels were significantly lower, and triglyceride, albumin, SOD and colostral IgG levels were\rsignificantly higher in cows with a BCS of ?3.5.\rIt was concluded that a BCS change of <0.5 from the drying period to parturition and a postpartum\rBCS of ?3.5 in dairy cows would be beneficial for better management of the metabolic process,\roxidative stress and high colostral IgG level.