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Öğe A broad assessment of Eremurus spectabilis M. Bieb: chemical and elemental composition, total phenolic and antimicrobial activity analysis, and quantum chemical calculations of radical scavenging potential(Taylor & Francis Ltd, 2024) Tegin, Ibrahim; Hallac, Bulent; Sabanci, Nazmiye; Sadik, Betul; Fidan, Mehmet; Yabalak, ErdalEremurus spectabilis M. Bieb was extensively investigated experimentally and theoretically, including the antioxidant properties of compounds such as flavonoids, hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, and organic acids. Antioxidant activity was investigated using the Density Functional Theory (DFT) method based on three known mechanisms: hydrogen atom transfer (HAT), single electron transfer followed by proton transfer (SET-PT), and sequential proton loss electron transfer (SPLET). Subcritical water extraction (SWE), soxhlet extraction (SE), and solvent extraction (SOE) techniques were applied in the extraction process. Malic acid was the major compound with an extract concentration of 38,532.84 +/- 1849.58 mu g analyte/kg, total phenolics, and free radical scavenging activity were 10.67 mg gallic acid/mL extract and 73.89% per mg/mL extract, respectively. P, Fe, Na, Mg, K, and Ca were the main elements. The antibacterial activity of E. spectabilis against seven bacteria was evaluated, and it was found to be higher than the commercial antibiotics P10 and AMC30.Öğe A New Record for The Flora of Türkiye: Cousinia mazu-shirinensis Rech.f(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Fidan, Mehmet; Pinar, Suleyman Mesut; Eroglu, Huseyin; Armagan, MetinCousinia mazu-shirinensis taxon was recorded for the first time in the Flora of T & uuml;rkiye from & Scedil;& imath;rnak province, located in the southeast of T & uuml;rkiye. The morphological characteristics of the C. mazu-shirinensis, the map of the distribution area, and the photographs of its natural habitat are also presented in the study.Öğe A new species of Gladiolus from Southeastern Turkiye, Gladiolus siirtensis (Iridaceae, Crocoideae)(Magnolia Press, 2023) Pinar, Suleyman Mesut; Eroglu, Huseyin; Fidan, MehmetGladiolus siirtensis (Iridaceae) is described as a new species from the Pervari district, Siirt province in the southeast of Turkiye. Gladiolus siirtensis is similar to G. humilis, but it is easily distinguished by its shorter stem, oblong or oblanceolate first leaves, dense spike with secund flower arrangement, absence of fusiform blotch on the lower segments of perigone, shorter anthers, style and stigma lobes and obovoid capsule. A detailed morphological study, including pollen and seed surface characteristics was undertaken for the new species. A complete morphological and palynological description is provided, including distribution data of the new species and related ones.Öğe An automatic identification method for the comparison of plant and honey pollen based on GLCM texture features and artificial neural network(Taylor & Francis As, 2013) Kaya, Yilmaz; Erez, Mehmet Emre; Karabacak, Osman; Kayci, Lokman; Fidan, MehmetPollen grains vary in colour and shape and can be detected in honey used as a way of identifying nectar sources. Accurate differentiation between pollen grains record is hampered by the combination of poor taxonomic resolution in pollen identification and the high species diversity of many families. Pollen identification determines the origin and the quality of the honey product, but this indefiniteness is also a big challenge for the beekeepers. This study aimed to develop effective, accurate, rapid and non-destructive analysis methods for pollen classification in honey. Ten different pollen grains of plant species were used for the estimation. GLCM (grey level co-occurrence matrix) texture features and ANN (artificial neural network) were used for the identification of pollen grains in honey by the reference of plant species pollen. GLCM has been calculated in four different angles and offsets for the pollen of the plant and the honey samples. Each angle and offset pair includes five features. At the final step, features were classified using the ANN method; the success of estimation with ANN was 88.00%. These findings suggest that the texture parameters can be useful in identification of the pollen types in honey products.Öğe Antioxidant capacity and phylogenetic analysis of twenty native grape cultivars in Siirt province, Turkey(2018) Fidan, Mehmet; Erez, Mehmet Emre; İnal, Behçet; Pınar, Süleyman Mesut; Altıntaş, SerdarThe quality of grape cultivars not only depends on the grape cultivar but also is influenced by the molecular concepts and agro-climatic factors. For this purpose, four different grape cultivars were collected from five different locations in Siirt province (Turkey). Totally twenty different grape cultivars were investigated. In the present study, the antioxidant activity (total phenolic, flavonoid, proanthocyanidin content, DPPH and FRAP activity) in seeds were indicated and phylogenetic analysis (cpDNA;trnL-F region) of twenty native grape cultivars were investigated to construct their phylogenetic tree. According to reported data on antioxidant activity and content of phytochemicals, all cultivars exhibited different values from each other, but Rutik and Gadüv cultivars were found as significantly higher in comparison to others. According to bioinformatics analysis, twenty grape cultivars were distributed into six different major groups. Rutik and Sevkeye cultivars exhibit significant distinction from other grape cultivars. The phylogenetic analysis was also associated and supported with the results of obtained data from bioactivity. The bioactivity and phylogenetic analysis were firstly identified and quantified in these grape cultivars, however, with regard to obtained data from the current study, the grape cultivars grown in Siirt province were indicated significant and valuable results and as a result, these cultivars have to be evaluated before extinction.Öğe The antioxidant capacity, total phenolic content and phenolic compounds of Plantago coronopus L. subsp. coronopus in naturally distributed in Akdoğmuş-Siirt(IEEE, 2018) Canpolat, Gurbet; Teğin, İbrahim; Fidan, MehmetName probably for Plantago coronopus L.; in reference to the shape of basal leaves [1]. Plantago coronopus, commonly known as buckshorn banana (“diabelha” in Portugal), is a medical halophyte belonging to the Plantago L., the largest of the Plantaginaceae family [2]. Plantago will be those the vast majority paramount variety of the Plantaginaceae crew, What's more, will be utilized within customary prescription around the reality for separate purposes. Plantago coronopus L., Plantago major L., Plantago media L. also Plantago lanceolata L. would a large portion regularly utilized species of Plantago for a customary solution to turkey [3]. There would 28 taxa of the class Plantago did turkey and two of them are endemic [4]. In this study, extracts of Plantago coronopus L. subsp. the coronopus taxon was prepared with methanol (%80) solvent. Total phenolic contents were assessed by Folin Ciocalteau's method and were found as 32.66 mg gallic acid /mL extract. The antioxidant activity was determined by using DPPH methods as 87.26%. And also, the chemical composition of the phenolic compounds was determined with LC-MS/MS instrument. As a result, 12 phenolic compounds were quantitatively detect, among the 27 phenolic compounds analyzed. These; Quinic acid (618±29.66 mg g -1 ), Malic acid (809.88±42.92 mgg -1 ), tr-Aconitic acid (4.49±0.22 mgg -1 ), Gallic acid (2.73±0.14 mgg -1 ), Protocatechuic acid (24.11±1.73 mgg -1 ), Tannic acid (33.01±1.68 mg g -l ), tr-Coffeic acid (11.06±0.58 mg g -l ), Vanilin (9.18±0.45 mgg -1 ), p-Coumaric acid (61.16±3.12 mgg -1 ), Kaempferol (43.64±2.27 mgg -1 ), Luteolin (5.35±0.37 mgg -1 ) and Apigenin (8.27±0.44 mgg -1 ).Öğe The antioxidant capacity, total phenolic content and phenolic compounds of Spergularia rubra (L.) J. Presl & C. Presl in naturally distributed five different saline areas in Siirt province(IEEE, 2018) Tegin, Ibrahim; Canpolat, Gurbet; Fidan, MehmetSpergularia rubra has a very wide spread in the world. Infusion of S. rubra is used for diuretic purposes. Although widely used, the antioxidant activity of this species has not been evaluated and its chemical composition is not known [1]. In this study, antioxidant activity (DPPH %), total phenolic content (Folin Ciocalteau method) and phenolic compounds profile (LC-MS/MS) of methanolic extract of Spergularia rubra plant that collected from five different localities in Siirt were analyzed. According to the results of the analysis, the total phenolic content range from 4.33 to 13.16 mg gallic acid/mL extract. The highest antioxidant activity value was found as 77.70 %, on the other hand, the lowest was found as 57.32 %. As a result of the LC- MS/MS analysis for Cöl (Bayraktepe), location, 18 phenolic compounds were quantitatively and qualitatively determined, among the 27 phenolic compounds. The all values obtained (μg analyte/kg extract) were as follows; Quinic acid (4788.53±229.85), Malic acid (9618.56±509.78), tr-Aconitic acid (96.76±4.74), Gallic acid (141.54±7.22), Protocatechuic acid (56.17±2.86), Tannic acid (6068.41±309.49), tr-Coffeic acid (2.55±0.13), Vanilin (30.02±1.47), p-Coumaric acid (48.64±2.48), Rutin (1.13±0.05), Hyperoside (4.47±0.2), 4-OH benzoic acid (9.26±0.4), Salicylic acid (13.3±0.6) Kaempferol (63.68±3.31), Naringenin (2.33±0.12), Luteolin (35.12±2.42) Apigenin (35.14±1.86) and Rhamnetin (29.28±1.78).Öğe Antioxidant, antimicrobial, cytotoxic and protective effects of truffles(Academic Press Inc Elsevier Science, 2022) Fidan, Mehmet; Ali, Muhammad Muddassir; Erez, Mehmet Emre; Cigerci, Ibrahim Hakki; Ozdemir, Sadin; Sen, FatihFungi can be used as a potent chemotherapeutic agent to treat various cancers. In current study acetone and methanol extracts of Terfezia claveryi, Terfezia boudieri, Terfezia olbiensis, Picoa lefebvrei, Picoa juniperi were used to assess total phenolic contents, antioxidant activity, ion-chelating impact, antimicrobial activity, the cytotoxic and protective effects. Both methanol and acetone extracts of T. boudieri had the highest FRAP and DPPH scavenging abilities. Dose-dependent increased ion-chelating impact of all tested truffles species was found. Extracts of T. boudieri, T. claveryi, and T. albiensis exhibited higher antimicrobial activities. T. claveryi and T. boudieri showed the highest protective effects against H2O2-induced genotoxicity (P < 0.05), in S. cerevisiae BY4741. The least protective effect was showed by the acetone extracts of T. olbiensis (144 +/- 8); methanol ex-tracts of P. lefebvrei (140 +/- 8) and P. juniperi (140 +/- 10). MCF 7 cells showed more sensitivity against to methanol extracts of T. boudieri at 10-100 mu g/mL concentrations. HepG2 cells showed more sensitivity against the methanolic extracts of T. boudieri at both doses. Overall, P. lefebvrei and P. juniperi extracts had the least cytotoxic effects. The species of Terfezia exhibit significant protective effects against DNA damage and also have the potential of cytotoxicity effects.Öğe Assessment of biological activity and element analysis of psylliostachys spicata (willd.) Nevski(2018) Fidan, MehmetThis study was conducted to determinethe antioxidant activity, total phenolic and flavonoid contents and also detection of phenolic compounds and element composition of Psylliostachys spicata by extraction of two different solvents (ethanol 80% andpure water).18 different phenolic compounds and 19 elements were determined from the P. spicata which collected from the Akdoğmuş salt area of Siirt province (Turkey).Total phenolic content was determined as 30.65 mg mL-1and 6.39 mg mL-1in ethanol and water extracts, respectively. The total flavonoid content was found as73.29 mg mL-1and 19.89mg mL-1in ethanol and water extract. The antioxidant activity wasdetermined by DPPH assay. The inhibition rates in 1mg mL-1concentration in ethanol and water extracts were determined as 93.91% and 41.73%, respectively. The obtained IC50 value was 0.058 mg mL-1for ethanol and 1.196 mg mL-1for water extract. The FRAP assay of P. spicata was determined as 774.440 mM mL-1mM mL-1in the ethanol and 74.074 mM mL-1in the water extract. According to the results of element analysis,P. spicata has high antioxidant capacity and potential to be used as an accumulator plant for halophytic areas in the future.Öğe An Assessment on the Floristic Variety of Province of Şırnak-Turkey In Terms of Botanical Tourism(ST. KLIMENT OHRIDSKI UNIVERSITY PRESS SOFIA. (Social Sciences Researches in the Globalizing World), 2018) Çelik, Sedat; İştin, Aysen Ercan; Rüstemoğlu, Mustafa; Fidan, Mehmet…Öğe An Automatic Identification Method for the Comparison of Plant and Honey Pollens Based On GLCM Texture Features and Artificial Neural Network.Grana, (2013) 52(1): 71-77.(2013) Kaya, Yılmaz; Erez, Emre; Karabacak, Osman; Kaycı, Lokman; Fidan, MehmetPollen grains vary in colour and shape and can be detected in honey used as a way of identifying nectar sources. Accurate differentiation between pollen grains record is hampered by the combination of poor taxonomic resolution in pollen identification and the high species diversity of many families. Pollen identification determines the origin and the quality of the honey product, but this indefiniteness is also a big challenge for the beekeepers. This study aimed to develop effective, accurate, rapid and non-destructive analysis methods for pollen classification in honey. Ten different pollen grains of plant species were used for the estimation. GLCM (grey level co-occurrence matrix) texture features and ANN (artificial neural network) were used for the identification of pollen grains in honey by the reference of plant species pollen. GLCM has been calculated in four different angles and offsets for the pollen of the plant and the honey samples. Each angle and offset pair includes five features. At the final step, features were classified using the ANN method; the success of estimation with ANN was 88.00%. These findings suggest that the texture parameters can be useful in identification of the pollen types in honey products.Öğe Bellevalia behcetii sp nov (Asparagaceae): a new species from South Eastern Anatolia, Turkey(Magnolia Press, 2016) Pinar, Suleyman Mesut; Eroglu, Huseyin; Fidan, MehmetBellevalia behcetii (Asparagaceae) is described and illustrated as a new species from Sirnak province in South Eastern Anatolia (Turkey). It is morphologically similar to B. paradoxa but easily differs in several morphological characters. Diagnostic morphological characters, a full description and detailed illustrations of the new species are provided. A quantitative morphometric analysis was carried out to check the diagnostic characters of Bellevalia behcetii with respect to morphologically related species. The somatic chromosome number was determined as 2n = 2x = 8 in B. behcetii. In addition, the ecology and phenology of the new species, as well as its etymology and the pollen characteristics, are presented and discussed. The conservation status of B. behcetii was assessed according to IUCN protocol. A distribution map of B. behcetii and related species is also presented.Öğe Bellevalia sasonii (Asparagaceae): a new species from Turkey(Magnolia Press, 2019) Fidan, MehmetBellevalia sasonii is herein described as a new species from south-eastern Anatolia, distributed only in the Sason district, in the province of Batman (Turkey), where it grows in oak forest clearances. B.sasonii is morphologically similar to B. malatyaensis, from which can be distinguished by the bulb, leaf, scape, pedicel, bud lobes and capsule characters. A full description and detailed photographs of this new species are provided. In addition, the ecology and phenology of the new species, as well as its etymology and pollen characteristics, are presented and discussed. According to the risk categories of the International Union for Conservation of Nature (IUCN), B. sasonii was assessed as Endangered (EN). An updated distribution map of B. sasonii and related species are presented.Öğe Characterization of Multifloral Honeys of Pervari Region with Different Properties(2015) Erez, Mehmet Emre; Karabacak, Osman; Kayci, Lokman; Fidan, Mehmet; Kaya, YılmazThe quality of honey from Pervari region was almost known by all over the country in Turkey. This study was undertaken to determine (i) physico-chemical parameters, (ii) antimicrobial analysis and (iii) pollen estimation method with expert computer system obtained from three different sites of Pervari region (Siirt/Turkey). For physico-chemical parameters; moisture, free acidity, diastase activity, hydroxyl methyl furfural (HMF), invert sugar, ash, commercial glucose and proline analysis were examined. For anti-microbial analysis disc dilution method were studied on six different bacteria species. For pollen analysis; different and new expert computer system was used for comparison of pollen of plants and honey samples. The aim of the study was to evaluate the properties of multi floral honey determined from three different locations in the same region and the way to understand to which plants were visited by the bees with comparing of pollen grains of flowers and honey by using the expert computer system. Honey samples of Pervari region were of acceptable quality based on recommended criteria of Turkish Food Codex and International Honey Commission.Öğe Chazaria incarnata (Freyer, 1838) Türünün Biyolojisi ve Yayılışı Hakkında Notlar (Lepidoptera, Noctuidae)(2010) Kayci, Lokman; Fidan, MehmetNotes on the Biology of Chazaria incarnata (Freyer, 1838) (Lepidoptera, Noctuidae). Cesa News 59: 55-59, 6 figs.[in Turkish] In this paper, new record of the larval food-plant, Silene compacta (Caryophyllaceae), and the provincial distribution of Chazaria incarnata (Freyer, 1838) are given.Öğe Comprehensive appraisement of antioxidant potential and phytochemical profile of native botanicals from Turkey(Springer, 2019) Erez, Mehmet Emre; Dalar, Abdullah; Fidan, Mehmet; Pinar, Suleyman MesutThis study presents the first comprehensive evaluation of antioxidant potential and phytochemical profiling of nine plant taxa utilized in southeastern and eastern parts of Turkey for various medicinal purposes, in order to contribute the scientific database, which was focused on exploring novel candidates of antioxidant agents from natural sources for industry and complementary medicine. Sequential extracts were prepared from plant materials were prepared through treating different solvents (acetone, ethanol, and water) based on polarity index. According to antioxidant assays via hydrogen atom transfer (HAT) and single atom transfer (SET) mechanisms, acetone extracts were found as the efficient antioxidant sources. Chromatographic studies revealed that phenolic compounds (mainly 5-O-Caffeoylquinic acid, luteolin hexoside, quercetin and rutin) were characterized as major hydrophilic phytochemical compounds and fatty acids (princibally linolenic, palmitic, and linoleic acids) were found as the major volatile compounds that dominantly contribute to the pronounced antioxidant capacities. According to the chemical structure, catechol moiety containing plant extracts had superior antioxidant capacity both for HAT and SET mechanisms. Data obtained from this study showed that among native botanicals, Crepis hakkarica, Crepis gemicii, Laserpitium carduchorum and Hedysarum vanaense might serve bioactive antioxidant agents both for industrial and pharmaceutical use.Öğe Cousinia gigantosphaera Rech, f. (Asteraceae-Compositae); Türkiye’nin Güneydoğu Anadolu Bölgesi’nden Yeni Bir Bitki Türü Kaydı(2019) Fidan, Mehmet; Pınar, Süleyman Mesut; Eroğlu, HüseyinTürkiye’nin Güneydoğu Anadolu Bölgesi’nde yer alan Şırnak ilinde 2018-2019 yılları arasında Tarım ve OrmanBakanlığı, Doğa Koruma ve Milli Parklar Genel Müdürlüğü kontrolünde “Şırnak İlinin Karasal ve İç Su EkosistemleriBiyolojik Çeşitlilik ve Envanter İzleme Projesi” yapılmıştır. Bu proje kapsamında yapılan arazi çalışmalarında toplananCousinia cinsine ait bazı örneklerin Türkiye’de bulunan taksonlardan farklılık gösterdiği belirlenmiştir. Bu örneklerindetaylı ve karşılaştırmalı teşhisi ile Cousinia gigantosphaera Rech.f olduğu anlaşılmış ve Türkiye’den (Şırnak,Beytüşşebap) ilk defa kayıt altına alınmıştır.Öğe Crocus biflorus subsp. pseudonubigena Üzerine Sistematik Bir Araştırma(International Engineering and Science Symposium (IESS), 2019) Fidan, Mehmet; Karaismailoğlu, Mehmet Cengiz; Sırma, Erkan; Yılmaz, Merve…Öğe Cytotaxonomy of Eight Thlaspi L. Sensu Lato (Brassicaceae) Species Endemic to Turkey(Univ Tokyo Cytologia, 2021) Karaismailoglu, Mehmet Cengiz; Fidan, MehmetIn this work, the karyomorphology of eight endemic Thlaspi species (T. cariense, T. violascens, T. densiflorum, T. cataonicum, T. elegans, T. rosulare, T. lilacinum and T. aghricum) has been studied for the first time. The chromosome number is defined to be 2n=2x= 12 in T. cataonicum, 2n=2x= 14 in T. violascens, T. densiflorum, T. lilacinum, 2n=2x= 16 in T. cariense, 2n=2x= 18 in T. elegans, T. rosulare, and 2n=2x= 20 in T. aghricum. It is concluded that generally, species have symmetrical karyotypes, considering the asymmetry indices. The karyotypes of all the species examined have shown variation. Most of the chromosomes of the studied Thlaspi have centromeres at the median and submedian regions. Systematic and evolutionary perspectives of the studied species are debated as per chromosomal data.Öğe Determination of Biological Activity and Active Substances of Thecocarpus Carvifolius (BOISS.) Hedge & Lamond(Springer, 2021) Pinar, Suleyman Mesut; Erez, Mehmet Emre; Fidan, Mehmet; Erodlu, Huseyin; Dalar, AbdullahWe have studied the antioxidant activity and analyzed active substances of the aqueous and methanol extracts of endemic species Thecocarpus carvifolius Hedge and Lamond. The antioxidant activity was identified and assessed using the DPPH, FRAP and iron chelating capacity assay. Active substances were detection, total phenolic, flavonoids and volatile compounds were analyzed. According to DPPH analyzes, the inhibition percentage was 48.57% for aqueous extract and 76.34% for methanol extract. The FRAP reduction capacity was determined as 3.32 mg/mL for aqueous extract and 11.75 mg/mL FESO4 for methanol extract. The total concentrations of phenolics and flavonoids were calculated as 64.08 - 35.29 mu g/mL and 146.84 - 555.66 mu g/mL in aqueous and methanol extracts, respectively. Finally, five volatile compounds were detected in methanol extracts. The results indicated that the endemic species of T. carvifolius can be evaluated as a natural antioxidant plant.
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