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    Effects of Arbutin on Fatty Acid Levels of Erythrocyte and Serum in Wistar Albino Rats Treated with Potassium Bromate
    (Assoc Pharmaceutical Teachers India, 2022) Akkoyun, H. Turan; Aydin, Sevinc; Akkoyun, Mahire Bayramoglu; Bengu, Aydin Sukru; Ekin, Suat; Erdem, Sinem Aslan
    Background: In the presented study; the Effects of Arbutin (ARB) on the Rat Erythrocyte and serum fatty acid profile which is exposed to potassium bromate (KBrO3) were investigated. Materials and Methods: In this study, 32 Wistar albino rats weighing 250-300 g were used divided into 4 groups. Groups 1: control, group 2: KBrO3 (single dose 100 mg / kg gavage), group 3: ARB (50 mg / kg / day (ip) for 5 days), group 4: KBrO3 + ARB. At the end of the 5(th) day, alteration of fatty acid profile in erythrocyte and serum of rats in all groups was examined. Results: Rat serum essential fatty acid; palmitic acid (C16:0), myristic acid (C14:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), erythrocyte major fatty acids; palmitic acid (C16:0), myristic acid (C14:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), arachidic acid (C20:0), eicosenoic acid (C20:1), and lignoceric acid (C24:0). In addition, in our studied serum and erythrocytes; Total monounsaturated fatty acids (MUFA) varied between 8.91 +/- 0.53- 11.71 +/- 2.55 and 33.71 +/- 2.12- 37.11 +/- 2.12, respectively. It was determined that total polyunsaturated fatty acids (PUFA) varied between 5.90 +/- 1.29- 9.96 +/- 1.18 and 14.72 +/- 3.66- 22.13 +/- 4.82, respectively. Conclusion: In our study, alterations in fatty acid contents were observed, and results suggesting that arbutine affects the enzymes involved in Fatty acid metabolism and has an effect on fatty acid amounts.
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    Öğe
    In Vivo and in Vitro Regulatory Effect of Silibinin on Some Metabolic Enzyme Activities against Cobalt Induced Toxicity in Rats: A Biochemical Approach
    (Amer Chemical Soc, 2023) Temel, Yusuf; Akkoyun, H. Turan; Akkoyun, Mahire Bayramoglu; Karagozoglu, Fatma; Melek, Sule; Bengu, A. Sukru; Erdem, Sinem Aslan
    The study aimed to examine the in vivo inhibition effect of cobalt ion and silibinin on metabolic enzymes such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR), and glutathione S-transferase (GST) and their in vitro inhibition effect on 6PGD. Twenty-four Wistar Albino rats weighing approximately 250-300 g were used in the study. The rats were divided into 4 groups as group 1 (control): isotonic serum (0.5 mL i.p), group 2 (cobalt): (150 mg kg/day cobalt), group 3 (silibinin): (100 mg/kg/day silibinin), group 4 (cobalt + silibinin). As a result of the in vivo applications, a statistically significant decrease was observed in the activities of G6PD (p < 0.05), 6PGD (p < 0.05), GR (p < 0.05), and GST (p < 0.05) enzymes in the groups that were administered cobalt compared to the control group. It was also found that the activities of G6PD (p < 0.05), 6PGD (p > 0.05), GR (p > 0.05), and GST (p > 0.05) enzymes increased in groups that were administered cobalt + silibinin compared to the group that was administered cobalt. As for in vitro applications, it was found that different Co2+ ions inhibited 6PGD enzyme which was obtained as a result of purification with IC50 = 346.6 mu M value, while silibinin increased 6PGD enzyme activity within the concentration range of 100-750 mu M by 40%. As a result, it was found that cobalt ions had an inhibition effect on G6PD, GR, and GST enzymes, which are vitally important for living metabolism, in vitro and in vivo and inhibited 6PGD enzyme activity in vitro, and silibinin increased these enzyme activities in vivo and 6PGD enzyme activity both in vivo and in vitro and decreased the inhibition effect.