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    Evaluation of endothelial glycocalyx injury biomarkers in feline hemotropic mycoplasmosis
    (Nature Portfolio, 2024) Ider, Merve; Ceylan, Ceylan; Naseri, Amir; Ceylan, Onur; Durgut, Murat Kaan; Ok, Mahmut; Iyigun, Suleyman Serhat
    The present study aimed to investigate endothelial glycocalyx (eGCx) damage in cats with feline hemotropic mycoplasmosis caused by Mycoplasma haemofelis using selected biomarkers and to determine the diagnostic and prognostic significance of these biomarkers. The study included 25 cats with feline hemotropic mycoplasmosis and 10 healthy cats. Clinical examination, blood gas analysis, complete blood count, and biochemical analysis were performed. Hemotropic mycoplasmosis diagnosed by microscopic examination and molecularly confirmed by PCR targeting the Mycoplasma haemofelis 16s rRNA gene. To evaluate endothelial glycocalyx damage, syndecan-1, endothelin-1 (ET-1), asymmetric dimethylarginine (ADMA), and vascular endothelial growth factor-A (VEGF-A) concentrations were measured using cat-specific commercial ELISA kits. Of the cats with feline hemotropic mycoplasmosis, 14 (56%) survived and 11 (44%) died. While syndecan-1 and ET-1 concentrations were significantly higher in cats with hemotropic mycoplasmosis compared to the control group (p < 0.001), no statistically significant difference was found for ADMA and VEGF-A concentrations (p > 0.05). Endothelial glycocalyx biomarkers showed significant correlations with each other and with hematological parameters (p < 0.01). The results of the ROC analysis showed that ET-1 with area under the curve (AUC) of 0.821 (p < 0.01) and VEGF-A with AUC of 0.805 (p < 0.010) were found to be significant prognostic indicators. In conclusion, this study demonstrated that serum syndecan-1 and ET-1 can be used as diagnostic and serum ET-1 and VEGF-A as prognostic biomarkers in cats with hemotropic mycoplasmosis. Our results indicate the development of eGCx damage in feline hemotropic mycoplasmosis and suggest that glycocalyx disruption may contribute to the pathogenesis of the disease.
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    Öğe
    Evaluation of Some Blood Gas, Hemogram and Biochemical Parameters in Cats with Hemoplasmosis
    (2022) İder, Merve; Durgut, Murat Kaan; İyigün, Süleyman; Ceylan, Ceylan; Kılıçkaya, Merve Cansu
    The aim of this study was to evaluate some blood gases, hemogram, and biochemical parameters in cats with hemoplasmosis. Ten healthy and fifteen infected cats were enrolled in the study. 7 mL of blood sample was taken once from all cats into tubes with and without anticoagulant. Blood gases, complete blood count, and biochemical analyzes were performed from blood samples. While pH, HCO3 and BE levels of cats with hemoplasmosis were significantly lower than healthy cats, lactate levels were higher (p< 0.05). WBC, Mon, Gra, and RDW levels were found to be significantly higher than in healthy cats, while RBC, Hct, Hb, and PLT levels were found to be lower (p< 0.05). AST, T.Bil, D.Bil, P, TG, LDH, TP, and CK levels were significantly higher than healthy, while Alb and Ca levels and A:G ratio were found to be low (p< 0.05). As a result, it was determined that metabolic acidosis, hyperlactatemia, anemia, hypertriglyceridemia, hypoalbuminemia, hyperbilirubinemia developed in cats with hemoplasmosis. In addition, it can be concluded that the A:G ratio should be considered in the diagnosis of infected cats and it should be evaluated together with other diagnostic test results.