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    Öğe
    Determination of Seminal Characteristics in Turkish Aseel Roosters
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2023) Gunay, Ebuderda; Arici, Ramazan; Senlikci, Hatice; Yagcioglu, Selin; Eser, Ahmet; Demir, Kamber; Alkan, Serhat
    Ornamental poultry is a hobby that has been of interest for centuries. The history of ornamental poultry associations in Europe dates back to the 19th century and to the Ottoman period in Turkiye. One of the most popular ornamental poultry species is Aseel roosters. Aseel roosters are indigenous of Pakistan and India, and they have been bred for competition during the Ottoman period. This study aims to determine the spermatological characteristics of Turkish Aseel roosters. In the study, 10 Aseel roosters were used, and semen was collected by the abdominal massage method twice a week. The sperm motility was estimated by a hot plate phase-contrast microscope under 400 ' magnification. The sperm concentration of each ejaculate was determined by hemocytometer and percentages of viable, dead, and abnormal spermatozoa was calculated using eosin-nigrosine staining. Acrosome membrane integrity of rooster spermatozoa were assessed using fluorescein isothiocyanate-conjugated peanut agglutin (FITC-PNA). Spermatozoa membrane functionality was assessed with the hypoosmotic (HOS) test. The spermatological data obtained as a result of the experiment are as follows; ejaculate volume average 308.49 +/- 12.14 mu L, spermatozoa motility 89.66 +/- 0.47%, spermatozoa concentration 2.39 +/- 0.10x109/mL, The general total morphological defect rate 17.19 +/- 0.75%, viability 85.45 +/- 0.88%, acrosome integrity rates 98.26 +/- 0.09%, and pH 7.81 +/- 0.02.
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    Öğe
    Effects of Resveratrol-Loaded Cyclodextrin on the Quality Characteristics of Ram Spermatozoa Following Cryopreservation
    (Mdpi, 2024) Eser, Ahmet; Yagcioglu, Selin; Arici, Ramazan; Demir, Kamber; Ak, Kemal
    This study investigated the effects of pure and methyl-beta-cyclodextrin loaded forms of resveratrol (10 mu g/mL, 20 mu g/mL, and 40 mu g/mL) on ram sperm functions post-thawing. Semen samples were pooled and divided into ten groups: Control, RES10, RES20, RES40, CD10, CD20, CD40, RLC10, RLC20, and RLC40. The groups were pre-diluted with media containing the group-specific chemicals, followed by 15 min of incubation, dilution, and freezing. To assess the effects of the chemicals, a post-thaw sperm quality assessment was conducted. Motility and other velocity parameters were evaluated using computer-assisted semen analysis. The functional integrity of spermatozoa membranes was assessed with the hypo-osmotic swelling test, and the capacitation status of spermatozoa was determined through fluorescent microscopic evaluation. Additionally, flow cytometry was used to evaluate mitochondrial activity, oxidative stress, and the integrity of the sperm membrane and acrosome. The results indicated that cyclodextrin adversely affected sperm functions following freezing-thawing, notably increasing the rate of spermatozoa exhibiting pre-capacitation and mitochondrial activity by approximately 34% and 16%, respectively (p < 0.05). It was found that 20 g/mL resveratrol prevented pre-capacitation (p < 0.05). Both resveratrol and resveratrol-loaded cyclodextrin groups improved post-thaw sperm qualities overall, demonstrating their utility for freezing ram semen. However, higher concentrations of resveratrol were found to negatively impact sperm functions.