Yazar "Coskun, Nuvit" seçeneğine göre listele

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    Endless variety for bovine virus diarrhea viruses: new members of a novel subgroup into Pestivirus A from Turkey
    (Springer, 2019) Oguzoglu, Tuba Cigdem; Koc, Bahattin Taylan; Coskun, Nuvit; Dogan, Firat; Duran-Yelken, Selda
    As ubiquitous pathogens, bovine virus diarrhea viruses (BVDVs) in cattle have been reported several times in Turkey. Over time, the frequency and importance of this infection has increased for the livestock industries. A total of 1291 animals were sampled from a dairy herd in Turkey suspected of BVDV clinical signs, for instance, reproductive failures (abortion, congenital malformations in calves, repeat breeding, etc.) and interdigital phlegmon in adult animals. Reverse transcription polymerase chain reactions (RT-PCRs) were made by using targeted 5' untranslated region (UTR), E2, and NS2-3 pestiviral gene region primers for antigen ELISA-positive samples (n = 20). The obtained amplicons were sequenced. Sequence results showed the presence of a new subgroup in Pe.stivirus A species. This paper describes the nucleotide sequences of a new BVDV 1 (BVDV 1v) subgroup member.
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    Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2019) Karayel Hacioglu, Ilke; Coskun, Nuvit; Duran Yelken, Selda; Sevinc, Secil; Alkan, Feray
    Bovine respiratory disease (BRD) causes economic losses related to a reduction in weight gain of affected animals, veterinary treatment costs, death, etc. One of the important respiratory tract disease viruses is the bovine respiratory syncytial virus (BRSV). In this study, it is aimed to report the molecular characterization of detected BRSVs. Therefore, nasal samples from three calves in a herd seen severe respiratory disorders were examined for BRSV and other possible viral etiological agents by PCRs and the amplicons were sequenced. In the phylogenetic tree, BRSV circulating in this herd is clustered with the genetic subgroup III BRSVs deposited in GenBank from some other countries. This study on the molecular characterization of BRSV circulating in calves would contribute for future studies on the epidemiology of this infection and the development and/or choice the effective vaccines in Turkey.
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    Öğe
    The genotype determination and molecular characterization of bovine leukemia virus in Turkey
    (Univ Zagreb Vet Faculty, 2021) Alkan, Feray; Karayel-Hacioglu, Ilke; Yelken, Selda Duran; Coskun, Nuvit
    Bovine leukemia virus (BLV) infection is widespread worldwide and causes significant economic losses in the livestock industry. In this study, to identify the genotypes and to investigate the molecular characterization of BLV strains, we analyzed 25 BLV strains from cattle housed in six herds located in several Turkish geographical regions. Phylogenetic analysis, based on a partial or full-length sequence of the env , gp51 gene of BLVs, showed that all Turkish BLVs belonged to genotype 1. The alignment of the deduced amino acid sequences demonstrated fourteen amino acid substitutions in different regions of the env gp51. This study updates our knowledge of BLV in Turkey. Further studies are needed to understand the molecular epidemiology and implement a comprehensive BLV control program in Turkey.