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    Determination of cytotoxic, anticholinesterase, antioxidant and antimicrobial activities of some wild mushroom species
    (Informa Healthcare, 2016) Dundar, Abdurrahman; Okumus, Veysi; Ozdemir, Sadin; Celik, Kadir Serdar; Boğa, Mehmet; Ozcagli, Eren
    In the performed study of methanol extraction of wild edible mushroom species; Agaricus arvensis, Agaricus campestris, Armillaria mellea, Fomes fomentarius, Coprinus micaceus, Coriolus versicolor and Lactarius deliciosus were examined for screening their cytotoxic, anticholinesterase, antioxidant and antimicrobial capacity. Phenolic acid composition of mushrooms was also analysed. L. deliciosus and F. fomentarius were generally showed the highest activities at antioxidant test systems (metal chelating, superoxide anion radical scavenging, total antioxidant, 2,2-diphenyl-1-picrylhydrazyl radical scavenging and reducing power activity tests). The highest activities at antimicrobial activity displayed by A. arvensis and as 18 ± 0.8 against to Staphylococcus aureus. The best IC50 values of mushroom methanol extracts at anticancer activities on HeLa and NRK-52E were 7.09 and 18.23 mg/mL exhibited by C. micaceus and A. campestris, respectively. The highest butyrylcholinesterase activity exhibited by L. deliciosus. Total amount of phenolic acids were found as 1,224.70 mg/kg at L. deliciosus. © 2016 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license.
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    Selective preconcentration of Lanthanum(III) by Coriolus versicolor immobilised on Amberlite XAD-4 and its determination by ICP-OES
    (Taylor & Francis Ltd, 2014) Ozdemir, Sadin; Okumus, Veysi; Dundar, Abdurrahman; Celik, Kadir Serdar; Yuksel, Uyan; Kilinc, Ersin
    Coriolus versicolor, a wood fungus, was immobilised on Amberlite XAD-4 and used as solid-phase biosorbent for preconcentrations of rare earth elements. La(III), Th(IV), U(IV) and Ce(III) were subjected to solid-phase extraction procedure. We observed that La(III) was selectively preconcentrated, while other ions remained in solution at pH 6.0. 5.0mL of 1.0molL(-1) HCl was used to elaute La(III) from column. 250mg of C. versicolor loaded on 1000mg of XAD-4 was optimised as solid-phase matrix. Concentrations of ions in solutions were determined by inductively coupled plasma- optical emission spectrometry (ICP-OES). The calibration plot after preconcentration was linear in the range from 1.0 to 50.0ngmL(-1) for La(III). Limit of detection was found as 0.27ngmL(-1) for La(III) by SPE method. Relative standard deviation was found lower than 6.7% for 1.0ngmL(-1) of La(III) solution (n=10). The sensitivity of ICP-OES was improved by a factor of 46.8. The applicability of the method was validated through the analysis of certified reference samples of tea (NCS ZC-73014) and spinach (NCS ZC-73013).