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    IDENTIFICATION OF Prunus persica ROOTSTOCKS TO RESISTANT TO ROOT-KNOT NEMATODES USING MARKER ASSISTED SELECTION
    (Parlar Scientific Publications (P S P), 2022) Basbuga, Selcuk; Basbuga, Sevil; Can, Canan; Atli, Halit Seyfettin
    Prunus is an important genus in the Prunoideae subfamily of the Rosaceae family. Some species such as peach (Prunus persica), almond (Prunus dulcis), plum (Prunus cerasifera, Prunus salicina, Prunus domestica) are used as rootstocks in stone fruit production because they can adapt to harsh climate and environmental conditions. It is aimed to produce rootstocks resistant to pathogens with classical and genetic breeding studies. Root knot nematodes slow down the active growth of Primus fruit trees such as peach and almond, reduce the resistance and cause yield loss. Recently, the use of nematicides in controling root knot nematodes has been restricted and that the damage has reached greater dimensions. For this reason, it has gained importance to use root-knot nematode-resistant rootstocks against nematodes in Prunus species. DNA marker systems that show tight linkage to genes (RMia, Ma and RMja genes) that provide resistance to root knot nematodes in Prunus species have been developed and it has been reported that these systems can be used with marker assisted selection (MAS) studies. In this study, resistance against root knot nematodes of F-1 hybrid individuals obtained from Ferragnes x GN22, Nemaguard x Ferragnes, GN22 x AB3, Nemaguard x AB3, Ferragnes x Nemaguard, Nemaguard x GN22 were determined. Three markers have been identified in the parent individuals [Nemaguard, GN22, Ferragnes, AB3 (Amygdalus orientalis Mill.) and GF67] that provide polymorphic discrimination, thus allowing selection of Ft individuals. As a result of PCR analyzes performed with these markers; 82 hybrid individuals were tested with the AMPP117 primer, and it was determined that 64 individuals were resistant and 18 individuals exhibited a sensitive profile. Additionaly, 77 hybrid individuals were screened with the LRR65 primer where 29 individuals were resistant and 48 individuals exhibited a sensitive profile. Totally, 86 hybrid individuals were tested with the SSRO5I12 primer, and that 34 individuals were resistant and 52 individuals exhibited a sensitive profile. As a result, the selection of peach hybrids resistant to root knot nematodes was achieved by using the markers whose usability was reported in marker assisted selection.