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    Horseshoe Adası (Antarktika) Çevresi Neritik Zonu Zooplankton ve İhtiyoplankton Türlerinin DNA Barkodlama Yöntemi Ile Belirlenmesi ve Morfolojik Özelliklerinin Tanımlanması
    (2022) Yaman, Yalçın; Kocabaş, Engin; Keleş, Murat; Kara, Alpaslan; Aymaz, Ramazan; İnceoğlu, Haşim; Kurt, Tuba Terbıyık
    Bu çalışma Horseshoe Adası neritik zonunda dağılım gösteren zooplanktonun çeşitlilik ve bolluk durumları ile morfolojik yapılarının tanımlanması ve DNA Barkodlama yöntemi ile türlerin en doğru şekilde tespit amacıyla tasarlanmıştır. Saha çalışmaları Antarktika Yarımadasında ve ülkemizin geçici bilim üssünün yer aldığı Horseshoe Adasının batı kıyılarında 10 istasyonda gerçekleştirilmiştir. Toplamda 447 adet örnek morfolojik olarak tanımlanmış ve barkodlanmıştır. Zooplankton bolluğunun nispeten düşük olduğu görülmüştür. Çalışma yapılan bölgedeki türlerin Copepoda grubu ağılıklı olduğu belirlenmiştir. Güney Okyanusunda baskın krill türü olan Euphasia superba?nın Horseshoe Adası neritik zonunda dağılım göstermediği, bu bölgede baskın krill türünün Euphausia crystallorophias olabileceği tespit edilmiştir.
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    Molecular characterization of fecundity-related gene regions in some of Türkiye's native sheep breeds
    (Tubitak Scientific & Technological Research Council Turkey, 2024) Aymaz, Ramazan; Ozdil, Fulya; Yaman, Yalcin
    In this study, the BMP15 and GDF9 gene regions were examined through DNA sequencing in some native sheep breeds of Turkiye (Kivircik, Karacabey Merino, Chios, Gokceada, cine capari, Awassi, and Karakacan). In the BMP15 exon 2 region, two single nucleotide polymorphisms (SNPs) were identified: one synonymous SNP, P248P (proline/proline), and one nonsynonymous SNP, L251P (leucine/proline), resulting in a missense mutation. In the GDF9 exon 1 region, only a nonsynonymous SNP, R87H (histidine/ arginine), was found. In the GDF9 exon 2 region, four SNPs were identified: two nonsynonymous SNPs, E241K (glutamate/lysine) and V332I (valine/isoleucine), and two synonymous SNPs, L159L (leucine/leucine) and E326E (glutamate/glutamate). The identified haplotypes in the GDF9 gene region showed a high D' (linkage disequilibrium) value, indicating a strong association. Furthermore, a complete correlation was observed between the E326E and V332I variants in the studied breeds. The Chios breed exhibited significantly higher frequencies of heterozygosity for the E326E and V332I variants compared to the Kivircik and Karacabey Merino breeds. The heterozygosity frequencies for the E326E and V332I variants in the Kivircik, Karacabey Merino, and Chios breeds were determined to be 0.08, 0.13, and 0.46, respectively. The higher frequency of heterozygosity observed in E326E and V332I variants within the Chios breed suggests their association with the trait of multiple births.
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    Morphological and Molecular Characterization of Neritic Zooplankton in Marguerite Bay, Antarctica
    (2025-01-01) Kara, Alpaslan; Kurt, Tuba Terbıyık; Yağci, Meral Apaydin; Veske, Erdinç; Kocabaş, Engin; İnceoğlu, Haşim; Keleş, Murat; Aymaz, Ramazan; Yaman, Yalçın
    This study details field investigations conducted during the 6th Turkish National Antarctic Science Expedition in February 2022, involving zooplankton sampling at ten stations along the western shores of Horseshoe Island, Marguerite Bay. Utilizing a WP-2 plankton net, both vertical and horizontal sampling methods were employed, with samples preserved for morphological and molecular analysis. Morphological assessments of collected zooplankton focused on detailed descriptions supported by digital imaging. Following Antarctic marine fieldwork, genetic research was initiated with DNA extraction from zooplankton specimens. Molecular analyses focused on amplifying mitochondrial gene regions. These mitochondrial DNA markers are recognized for species identification and phylogenetic investigations. This study was conducted by combining classical morphological assessment with mitochondrial DNA barcoding technology. As a result, molecular analyses of Calanoides acutus and Paralabidocera grandispina revealed high identity percentages (≥98%) when compared to reference sequences in the BOLD database, demonstrating successful species identification through mitochondrial DNA (mtDNA) barcoding. Detailed morphological features of these two species, as well as others, were documented with particular focus on the structure of the swimming legs and genital segments. The study aims to contribute to the understanding of zooplankton biodiversity in Antarctic marine ecosystems, providing preliminary insights into genetic diversity and potential cryptic species through molecular genetic techniques.
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    Ovine PAPPA2 gene coding variants are linked to decreased fecal egg shedding in native Turkish sheep naturally infected with gastrointestinal nematodes
    (Springer, 2023) Yaman, Yalcin; Bay, Veysel; Sevim, Semih; Aymaz, Ramazan; Keles, Murat; Onaldi, A. Taner; Ozuicli, Mehmet
    In this study, the association between PAPPA2 coding variants and gastrointestinal (GI) nematode fecal egg count (FEC) score in adult Turkish sheep was investigated. For this purpose, the FEC score was determined in adult sheep from six breeds: Karacabey Merino (n = 137), Kivircik (n = 116), Cine capari (n = 109), Karakacan (n = 102), Imroz (n = 73), and Chios (n = 50). Sheep were classified as shedders or non-shedders within breeds and flocks. The first group was the fecal egg shedders (> 50 per gram of feces), and the second group was the no fecal egg shedders (<= 50 per gram of feces). The exon 1, exon 2, exon 5, exon 7, and a part of 5 ' UTR of the ovine PAPPA2 gene were genotyped by Sanger sequencing of these two groups. Fourteen synonymous and three non-synonymous single-nucleotide polymorphisms (SNPs) were found. The non-synonymous SNPs, D109N, D391H, and L409R variants, are reported for the first time. Two haplotype blocks were constructed on exon 2 and exon 7. The specific haplotype, C(391)G(424)G(449)T(473)C(515)A(542) on the exon 2 that carries the 391H variant, was tested against four other common haplotypes. Our results indicate that C(391)G(424)G(449)T(473)C(515)A(542) haplotype was significantly associated with fecal egg shedding status in adult Turkish sheep (p-value, 0.044).