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    A SURVEY OF THE PREVALENCE AND GENOTYPES OF CRYPTOSPORIDIUM SPP. AND GIARDIA DUODENALIS IN SHELTER DOGS IN BATMAN, TURKEY
    (Trakia University, 2024) Aslan Çelik, Burçak; Yilmaz, R.; Çiftçi, T.; Çelik, Ö.Y.; Ayan, A.; Orunç Kilinç, Ö.; Akyildiz, G.
    Cryptosporidium spp. and Giardia duodenalis are opportunistic zoonotic protozoan parasites related to diarrhea in humans and many mammals. This study aimed to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in shelter dogs in Batman province. The animal material of the study consisted of 100 dogs of different breeds and sexes. Fresh fecal samples taken from the dogs were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia. DNA extraction, nested PCR analysis, and sequence analysis were then performed. As a result of the analyses, all samples were negative for Cryptosporidium spp., while Giardia duodenalis was positive in 2% (2/100) of two female dogs less than one-year-old. Sequence analyses of PCR-positive samples showed that the samples overlapped with assemblage C and D samples. Although these results show that shelter dogs in Batman province do not carry a risk for humans in terms of Cryptosporidium spp. and Giardia duodenalis, it is recommended that repeated faecal examinations should be carried out as much as possible to determine the possible role of these parasites in human transmission. © 2024, Trakia University. All rights reserved.
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    First detection of Ehrlichia chaffeensis, Ehrlichia canis, and Anaplasma ovis in Rhipicephalus bursa ticks collected from sheep, Turkey
    (Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, A.; Celik, B. Aslan; Celik, O. Y.; Kilinc, O. Orunc; Akyildiz, G.; Yilmaz, A. B.; Ipek, D. N. Sayin
    Anaplasmosis and ehrlichiosis are important tick -borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co -infections in sheep in Turkey.
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    Molecular detection and subtype distribution of Blastocystis sp. from shelter dogs and cats in Van, Turkey: First report of ST10 in cats and ST1, ST10 and ST30 in dogs
    (Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, A.; Celik, B. Aslan; Celik, O. Y.; Yilmaz, A. B.; Kilinc, O. Orunc; Ayan, O. Oktay
    Blastocystis is an intestinal protist commonly found in humans and many different animal species. It is probably the most common enteric parasite with an estimated one billion infections worldwide. The fecal materials for this study were collected from 100 cats and 200 dogs different age and sex in shelter in Van, Turkey. DNA extraction, PCR amplification and sequence analysis were performed on the fecal samples. As a result, a prevalence of 1% (1/100) in cats and 1.5% (3/200) in dogs was detected. The prevalence was higher in both cats and dogs, in age groups younger than one year and in females according to gender. Sequence analysis revealed Blastocystis sp. ST10 in cats and Blastocystis sp. ST1, ST10 and ST30 in dogs. The sequences obtained were deposited in Genbank. In conclusion, stray cats and dogs may be a source of infection for other cats and dogs, and the detection of zoonotic ST1 in dogs suggests that dogs may be a reservoir for human infection.
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    Occurence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in sheep in Siirt, Turkey
    (Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2023) Celik, B. Aslan; Celik, O. Y.; Ayan, A.; Kilinc, O. Orunc; Akyildiz, G.; Irak, K.; Selcuk, M. A.
    Cryptosporidium spp., and Giardia duodenalis are intestinal protozoan parasites known to infect humans and various animals and cause diarrhea. This study aimed at determining the prevalence and genotype of Cryptosporidium spp. and Giardia duodenalis in sheep in different locations of Siirt province. The fecal material for this study was collected from 500 sheep in different locations of Siirt province, Turkey. Fecal samples obtained from sheep were examined for Cryptosporidium spp. by Kinyoun Acid Fast staining and the Nested PCR method. Microscopic and Nested PCR methods revealed a prevalence of 2.4% (12/500) and 3.6% (18/500), respectively. Sequence analysis revealed the presence of C. ryanae, C. andersoni, and zoonotic C. parvum. In terms of Giardia duodenalis, 8.4% (42/500) and 10.2% (51/500) prevalence was determined using Nativ-Lugol and Nested PCR methods, respectively. Using sequence analysis, zoonotic assemblages A and B as well as assemblages E and D were detected. As a result of this study, both the prevalence of Cryptosporidium spp. and Giardia duodenalis and the presence of species that appear to be host-specific, as well as those known to be zoonotic, were revealed. A large-scale study is needed to understand the impact of these agents on sheep farming and their consequences on human health.