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    Immobilization of Pectinex Ultra SP-L to produce galactooligosaccharides
    (Journal of Molecular Catalysis B: Enzymatic, 2007) Aslan Y., Tanrıseven A.
    Pectinex Ultra SP-L, a commercial enzyme preparation obtained from Aspergillus aculeatus, containing -galactosidase activity,was immobilized onto Eupergit C and was used for the production of galactooligosaccharides (GOS). Immobilization resulted in 100% binding yield and higher GOS yield (24%, w/v) with respect to soluble enzyme. Optimum conditions were not affected by immobilization, and optimum pH and temperature for free and immobilized enzyme were 4.0–5.0 and 55–60 ?C, respectively. Immobilized enzyme was more stable at high pH and temperatures. The amount of GOS produced from 30% (w/v) lactose solution using the free and immobilized enzyme was determined to be 12.8 and 15.8% (w/v) of the total sugar in the reaction mixture, respectively. The kinetic parameters for the free and immobilized enzyme were also determined. The immobilized Pectinex Ultra SP-L could be used for the production of galactooligosaccharides, since the immobilization yield is high (124%) and immobilized enzyme retains its activity for 20 days without any decrease.
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    Immobilization of Penicillium lilacinum dextranase to produce isomaltooligosaccharides from dextran
    (Biochemical Engineering Journal, 2007) Aslan Y., Tanrıseven A.
    Penicillium lilacinum dextranase was immobilized covalently onto Eupergit C and was used for the production of isomaltooligosaccharides. Immobilization resulted in 90% relative activity. The immobilized enzyme showed no decrease in activity for 20 batch reactions. Optimum conditions were not affected by immobilization, and optimum pH and temperature for free and immobilized enzyme were 4.5–5.5 and 30–35 ?C, respectively. Immobilized enzyme was more stable at low and high pH and high temperatures. The kinetic parameters for the free and immobilized enzyme were also determined. The immobilized enzyme could be used for the production of isomaltooligosaccharides, since the immobilization efficiency is high (90%) and immobilized enzyme retains its activity for 20 days without any decrease.