Yazar "Altintas, Serdar" seçeneğine göre listele

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    Effectiveness of manganese foliar spraying to mitigate salt stress in ornamental cabbage: Insights into morphological, physiological biochemical adaptations and mTERF gene responses
    (Elsevier, 2024) Altintas, Serdar; Yasemin, Sara; Catkin, Sedriye; Inal, Behcet
    Salinity has been the subject of research for many years. However, salt stress studies on ornamental plants have increased recently. It is very important to determine the responses of bedding flowers, which are widely used in landscaping, to salt stress, and to strengthen the defense against stress. Ornamental cabbage (Brassica oleareacea L.) is widely used bedding flower in winter. The aim of this study was determining the effects of foliar application of manganese (Mn) on morphological, physiological, biochemical, and molecular parameters in ornamental cabbage ('Pigeon Purple F10) 0 ) under salt stress. Plants were irrigated with 4 different irrigation solutions which containing 0 [control], 75, 150 and 300 mM NaCl concentrations and Mn (4000 ppm) was sprayed to the leaves on 0, 7th and 14th days of the salt stress application. The application of manganese did alleviate the negative effects of salinity on shoot fresh weight and shoot dry weight in 75 mM NaCl plants. Ion leakage for 0 and 75 mM NaCl decreased with foliar spraying of Mn, but relative water content of plants did not change with Mn application. Lipid peroxidation (7.6 %) and H2O2 2 O 2 (hydrogen peroxide) (21.3 %) contents increased with Mn foliar spraying. Superoxide dismutase (SOD) and peroxidase activity did not change with Mn application under salinity. The highest catalase activity obtained from 150 mM (13.81 Unit g- 1 leaf) and 300 mM NaCl (16.00 Unit g- 1 leaf) which treated with Mn. Root Na content was lower with Mntreated plants when compared control plants under salt stress. Foliar spraying of Mn significantly increased the K content of the plants. Furthermore, Na/K and Na/Ca ratios which are important indicators for salt stress also decreased by Mn application under saline conditions. In addition, m TERF genes were measured by using the q-RT PCR. In most cases, mTERF expression was slightly induced by salinity, while manganese application generally led to downregulation of mTERFs. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.
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    Evaluation of mTERF gene expressions in response to Phytophthora capsici infection in pepper (Capsicum annuum L.) cultivars
    (Academic Press Ltd- Elsevier Science Ltd, 2024) Altintas, Serdar; Kuru, Ibrahim Selcuk; Erez, Mehmet Emre; Catkin, Sedriye; Inal, Behcet; Bingol, Omer
    Despite resistance to abiotic stresses, some plant species may still show low resistance to pathogens. This biotic stress resistance can provide advantage and increase productivity in agricultural systems. However, while there is extensive research on the pathways involved in abiotic stress tolerance, the impact on the molecular mechanisms underlying pathogen resistance remains poorly understood. Among the pathogens , Phytophthora capsici , an oomycete, causes severe damage as plant pathogen, leading to blight and fruit rot in pepper and other commercially valuable crops. This study investigates the effects of Phytophthora capsici infection on highly resistant (CM334) and susceptible (Sera Demre) pepper varieties. We examined biochemical changes and gene expression across different vegetative periods (seedling, flowering, and fruiting stages). Specifically, we analyzed biochemical pathways and the mitochondrial transcription termination factor (mTERF) gene regions associated with pathogenicity. In pepper seedlings infected with P. capsici , peroxide levels increased at all developmental stages compared to control groups. Additionally, we observed a rise in H 2 O 2 content, a molecule involved in signal transduction, likely due to the oxidative stress caused by the pathogen. The expression patterns of mTERF genes varied significantly depending on both pathogen application and the developmental stage of the plants. In the seedling stage, inoculation with P. capcici resulted in decreased expression of TERF2, mTERF6, and mTERF8 genes in leaf parts of both resistant and susceptible varieties. However, mTERF28 expression showed a significant increase. Interestingly, mTERF14 and mTERF27 gene expressions increased in the resistant variety but decreased in the susceptible one. These findings, although not providing a complete picture of host resistance mechanisms, highlight the crucial role of specific plant genes in the defense pathways against Phytophthora capsici . This knowledge can contribute to selecting pepper varieties with sustainable resistance to this devastating pathogen.
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    Genetic diversity among some Asparagus species using rDNA ITS, cpDNA trnL intron sequence and screening for antioxidant activity
    (HARD Publishing Company, 2019) Altintas, Serdar; Pakyürek, Mine; Sensoy, Suat; Erez, M. Emre; Inal, Behcet
    Three species of genus Asparagus native to Lake Van Basin of Turkey were analyzed using internal transcribed spacer (ITS) and cpDNA trnL intron sequence. Furthermore, these species were screened for their antioxidant activity and total phenolic and flavonoid contents. Phylogenetic analysis based on ITS data revealed two clades: the first clade consisted of Asparagus persicus and Asparagus officinalis, and clade II consisted of Asparagus palaestinus. Similarly, phylogenetic analysis using trnL intron sequence revealed two clades. In term of total phenolic content and total flavonoid content, the highest value were measured for acetone extract of Asparagus persicus sample No. 2 and Asparagus persicus sample No. 6, respectively. The highest antioxidant activity using FRAP assay was found for Asparagus persicus sample No. 3. We concluded that these species could be useful for increasing genetic diversity among cultivated species and for breeding. © 2019, HARD Publishing Company. All rights reserved.
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    Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources
    (Galenos Publ House, 2022) Karatas, Merve Dilek; Hazrati, Nahid; Oguz, Ezgi; Ozmen, Canan Yuksel; Altintas, Serdar; Akcay, M. Emin; Ergul, Ali
    Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes (alleles). Pear (Pyrus communis L.) belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Ataturk Horticultural Central Research Institute-Yalova collection is considered as genes bank. Identification of the different features of this collection (fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.) is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR (Polymerase Chain Reaction)-based amplification of the S-allele regions (S-1, S-6, S-7, S-8) causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S-6 allele was the most prevalent one with 63% frequency, while the S-8 allele was the least common allele with a rate of 4%. In allele combinations, the SI-So allele combination was the most common allele combination with a rate of 18%. and trilateral allele combinations (S-1-S-6-S-7 and S-1-S-6-S-8) were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.
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    Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR
    (Galenos Publ House, 2023) Karatas, Merve Dilek; Hazrati, Nahid; Yuksel Ozmen, Canan; Hasanzadeh, Mohammad; Altintas, Serdar; Akcay, Mehmet Emin; Ergul, Ali
    Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Huryemez, Sah elmasi, Tokat, Demir elmasi etc.) obtained from the National Collection of Ataturk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as 'Partially Incompatible' and 12 genotypes involving 4 S-alleles were assigned 'Totally Incompatible'. No S-alleles were observed in 2 genotypes (Pancarlik and Huryemez) which exhibited 'Compatibility' status.
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    The comparison of phenolic compounds content, antioxidant capacity and molecular analysis of some selected Turkish pear genotypes
    (Parlar Scientific Publications, 2018) Ozrenk, Koray; Erez, Mehmet Emre; Altintas, Serdar; Inal, Behcet
    The current study was focused on molecular analysis using internal cpDNA trnL intron sequence, antioxidant activity, total phenolics and flavonoid contents of fifteen pyrus genotypes. Quite variations were determined in biochemical characteristics and molecular systematic analysis of genotypes evaluated. "Y. Karçin" genotype was detected as the best graded genotype based on the high contents of the total phenolic (7,92 mg GAE/g) and total flavonoid (4,50 mg RE/g) content. The obtained results showed that, genotypes could be categorized of six main clades with robust bootstrap values, which might be useful to improve genetic diversity among genotypes for breeding. © by PSP.
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    THE COMPARISON OF PHENOLIC COMPOUNDS CONTENT, ANTIOXIDANT CAPACITY AND MOLECULAR ANALYSIS OF SOME SELECTED TURKISH PEAR GENOTYPES
    (Parlar Scientific Publications (P S P), 2018) Ozrenk, Koray; Erez, Mehmet Emre; Altintas, Serdar; Inal, Behcet
    The current study was focused on molecular analysis using internal cpDNA trnL intron sequence, antioxidant activity, total phenolics and flavonoid contents of fifteen pyrus genotypes. Quite variations were determined in biochemical characteristics and molecular systematic analysis of genotypes evaluated. Y. Karcin genotype was detected as the best graded genotype based on the high contents of the total phenolic (7,92 mg GAE/g) and total flavonoid (4,50 mg RE/g) content. The obtained results showed that, genotypes could be categorized of six main clades with robust bootstrap values, which might be useful to improve genetic diversity among genotypes for breeding.