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Öğe Biofortification of Iron in Potato Through Rhizobacteria and Plant Growth Regulator(Springer, 2024) Mushtaq, Zain; Al-Ashkar, Ibrahim; Habib-ur-Rahman, Muhammad; Sabagh, Ayman El; Ilic, PredragMillions of people around the world have iron deficiency, which is one of several important nutritional disorders. Despite recent advances in the prevention and treatment of these deficiencies, it is estimated that over two billion individuals worldwide are at risk for vitamin A, iodine, and/or iron insufficiency. Many major health concerns can result from micronutrient deficits. Lack of iron, folate, B12, and A may cause anemia. Anemia causes weariness, weakness, breathlessness, and disorientation due to low red blood cell or hemoglobin levels. Biofortification, a way to make staple foods with more micronutrients, could help lower malnutrition. Plant growth-promoting rhizobacteria (PGPR) can boost the amount of iron in edible plant tissues by making it easier for iron to get to those tissues. A pot experiment was conducted at ISES, University of Agriculture, Faisalabad, to evaluate the impact of well-characterized rhizobacterial strains (Acinetobacter calcoaceticus and Bacillus simplex) alone and in combination with L-tryptophan (a plant growth regulator (PGR)) on agronomic, tuber, and nutritional attributes of potato. Results revealed that inoculation significantly enhanced the iron content of potato tubers compared to control plants. When rhizobacteria, L-tryptophan, and iron were added to the soil, the number of tubers grew by 68.24% compared to the control plants where neither PGPR nor PGR was applied. Biofortifying potatoes with microbes can help people get more micronutrients, especially in countries with few resources. These results showed that PGPR can help plants get more Fe from the soil if more Fe is added to the soil. These results provide support for the hypothesis that microbial aided biofortification in crops might reduce human micronutrient insufficiency, particularly in areas with little resources.Öğe Characterization of chromosomal segment substitution lines developed in the genetic background of rice variety K 343(Cellular and Molecular Biology Association, 2024) Niharika; Sharma, Manmohan; Salgotra, R.K.; Sharma, Mamta; Sharma, Richa; Ratnasekera, Disna; Al-Ashkar, IbrahimIn this study, BC3F2 convergent population [(K343*3/RML22 × K343*3/DHMAS) × K343] was constructed by marker-assisted backcross breeding using K343 as the recurrent parent. DHMAS and RML22 were used as donor parents for the rice blast resistance genes Pi54 and Pi9, respectively. The population was first characterized using GGT 2.0 software, which showed 96.7% of the recurrent genome recovery covering 13953.6 cM, while DHMAS and RML22 showed 1.6% (235.5 cM) and 1.2% (177.1 cM) introgression respectively. The chromosomal segment substitution lines (CSSLs) were then identified using CSSL Finder software. A total of 36 CSSLs were identified, including 22 for DHMAS/K343 and 14 for RML22/K343. Introgression rates for donor substituted segments in DHMAS/K343 CSSLs ranged from 0.54% to 5.99%, with donor coverage of 44.5%, while in RML22/K343 CSSLs, introgression rates ranged from 0.54% to 4.75%, with donor coverage of 24.5%. The identified CSSLs would be a valuable genetic pool and could be used as genomic resources for the discovery and mapping of important genes and QTLs in rice genetic improvement. © 2024 Cellular and Molecular Biology Association. All rights reserved.Öğe Enhancing Canola Yield and Photosynthesis under Water Stress with Hydrogel Polymers(Tech Science Press, 2024) Badr, Elham A.; Bakhoum, Gehan Sh.; Sadak, Mervat Sh.; Al-Ashkar, Ibrahim; Islam, Mohammad Sohidul; El Sabagh, Ayman; Abdelhamid, Magdi T.While Egypt's ' s canola production per unit area has recently grown, productivity remains low, necessitating increased productivity. Hydrogels are water -absorbent polymer compounds that can optimize irrigation schedules by increasing the soil's ' s ability to retain water. Accordingly, two fi eld experiments were conducted to examine hydrogel application to sandy soil on canola growth, biochemical aspects, yield, yield traits, and nutritional quality of yielded seeds grown under water deficit fi cit stress conditions. The experiments were conducted by arranging a split -plot layout in a randomized complete block design (RCBD) with three times replications of each treatment. While water stress at 75% or 50% of crop evapotranspiration (ETc) lowered chlorophyll a, chlorophyll b, carotenoids, and total pigments content, indole-3-acetic acid, plant development, seed yield, and oil and total carbohydrates of seed yield, hydrogel treatment enhanced all of the traits mentioned above. Furthermore, hydrogel enhanced to gather compatible solutes (proline, amino acids, total soluble sugars), phenolics content in leaves, seed protein, and crop water productivity, which increased while the plants were under water stress. The results revealed that the full irrigation (100%ETc) along with hydrogel compared to water -stressed (50%ETc) led to enhanced seed yield (kg ha -1 ), Oil (%), and Total carbohydrates (%) of rapeseed by 57.1%, 11.1% and 15.7%, respectively. Likewise, under water -stressed plots with hydrogel exhibited enhancement by 10.0%, 3.2% and 5.1% in seed yield (kg ha -1 ), oil (%), and total carbohydrates (%) of rapeseed by 57.1%, 11.1% and 15.7%, respectively compared to control. As a result, the use of hydrogel polymer will be a viable and practical solution for increasing agricultural output under water deficit fi cit stress situations.Öğe Exogenously applied nano-zinc oxide mitigates cadmium stress in Zea mays L. through modulation of physiochemical activities and nutrients homeostasis(Taylor & Francis Inc, 2024) Ahmed, Shakil; Ashraf, Sana; Yasin, Nasim Ahmad; Sardar, Rehana; Al-Ashkar, Ibrahim; Abdelhamid, Magdi T.; Sabagh, Ayman ElThe increasing levels of cadmium (Cd) pollution in agricultural soil reduces plant growth and yield. This study aims to determine the impact of green synthesized zinc oxide nanoparticles (ZnO-NPs) on the physiochemical activities, nutrition, growth, and yield of Zea mays L. under Cd stress conditions. For this purpose, ZnO-NPs (450 ppm and 600 ppm) synthesized from Syzygium aromaticum were applied through foliar spray to Z. mays and also used as seed priming agents. A significant decline in plant height (35.24%), biomass production (43.86%), mineral content, gas exchange attributes, and yield (37.62%) was observed in Cd-spiked plants compared to the control. While, 450 ppm ZnO-NPs primed seed increased plant height (18.46%), total chlorophyll (80.07%), improved ascorbic acid (25.10%), DPPH activity (26.66%), and soil mineral uptake (Mg+2 (38.86%), K+ (27.83%), and Zn+2 (43.68%) as compared to plants only spiked with Cd. On the contrary, the foliar-applied 450 ppm ZnO-NPs increased plant height (8.22%), total chlorophyll content (73.59%), ascorbic acid (21.39%), and DPPH activity (17.61%) and yield parameters; cob diameter (19.45%), and kernels numbers 6.35% enhanced compared to plants that were spiked only with Cd. The findings of the current study pave the way for safer and more cost-effective crop production in Cd-stressed soils by using green synthesized NPs and provide deep insights into the underlying mechanisms of NPs treatment at the molecular level to provide compelling evidence for the use of NPs in improving plant growth and yield. [GRAPHICS]Öğe Genotypic Divergences of Important Mungbean Varieties in Response to Salt Stress at Germination and Early Seedling Stage(Hard, 2024) Shaddam, Md. Obaidullah; Islam, M. R.; Ditta, Allah; Ismaan, Hassan Nuur; Iqbal, Muhammad Aamir; Al-Ashkar, Ibrahim; El Sabagh, AymanSalinity drastically hampers the germination and growth of legumes including mungbean which jeopardizes the food and nutritional security of the rising human population. An experiment entailing varying salinity levels (0, 50, and 150 Mm NaCl) was conducted to investigate the response of salinity on germination and seedling growth of four mungbean genotypes (BARI Mung-7, BARI Mung-8, BU Mug-2, and BU Mug-4). The germination indices such as percentage and rate of germination along with the coefficient of velocity (GP) of all mungbean genotypes were significantly reduced by the salt stress, especially of Binamoog-5 at 150 mM NaCl. Contrarily, BARI Mung-8 remained superior by recording better germination indices under all salinity levels. The same cultivar outperformed the rest of the cultivars based on the greatest weights (fresh and dry) and length of roots and shoots, while BU Mug-2 could not perform at par under a saline environment. Thus, it might be inferred that mungbean genotypes differ in their potential for salt endurance due to the germination vigor and seedling growth robustness and screening of salt-tolerant cultivars could mitigate the detrimental effects of salineÖğe Modern Breeding Approaches for Climate Change(Springer International Publishing, 2023) Afzal, Sadia; Mubeen, Muhammad; Hussain, Sajjad; Ali, Mazhar; Rashad Javeed, Hafiz Muhammad; Al-Ashkar, Ibrahim; Soufan, WalidClimate-smart agriculture is the emerging and sustainable option to mitigate the adverse effects of climate change (on crop adaptability) before it significantly influences global crop production. Crop development through modern breeding techniques, effective agronomic practices and exploitation of natural variability in neglected and popular crops are all good ways to meet future food demands. However, the rapidly changing environment requires technological interventions to improve crop climate resilience. Technological advances such as genome-edited transgenic plants, high-throughput phenotyping technologies combined with next-generation sequencing techniques, big data analytics and advances in modern breeding techniques help modern agriculture progress towards robotics or digital conversion to face future environmental adversaries. For example, speed breeding in combination with genomic and phenomic methods can lead to quicker identification of genetic factors and, as a result, speed up crop development programmes. Furthermore, combining next-generation interdisciplinary breeding platforms might open up new opportunities for developing climate-ready crops. Several integrated modern breeding platforms were created in the last few decades and are now employed worldwide. Africa and Asia have adopted these most frequently used crop improvement platforms with advanced techniques like multitrait association studies using genome-wide association studies (GWASs). These have permitted precise exploration of the genetic make-up of agricultural attributes in most crops. This chapter explores various ways to increase crop output by developing climate-resilient superior genotypes. Further, we discussed how combinatorial advanced breeding technologies and biotechnological approaches would be used for managing climate change’s consequences to promote crops with climate resilience. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.