Yazar "Akkoyun, H. T." seçeneğine göre listele

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    AMELIORATIVE EFFECT OF ASTAXANTHIN ON ISCHEMIA-REPERFUSION INJURY OF SKELETAL MUSCLES
    (Pakistan Agricultural Scientists Forum, 2022) Uyar, A.; Akkoyun, H. T.; Bengu, A. S.; Akkoyun, M. B.; Keles, O. f.; Atcali, T.; Melek, S.
    This experimental study aimed to investigate the ameliorative effect of astaxanthin (AST) on the prevention of skeletal muscle injury resulting from lower extremity ischemia/reperfusion (I/R). Twenty-eight (250-300g) male Wistar albino rats were divided into 4 groups as Control, I/R, I/R+AST and AST. In the control group, only anesthesia was induced for 2 h without I/R. In the I/R group, 2 h of reperfusion was facilitated following ischemia under anesthesia. For the I/R+AST group, 7 days prior to ischemia, 125 mg/kg AST was given through a gavage, and 2 h of ischemia and 2 h of reperfusion were facilitated under anesthesia. At the end of the study, blood and gastrocnemius muscle tissue samples were taken for biochemical, histopathological and immunohistochemical examinations. Compared to the control group, there were increased Malondialdehyde (MDA) levels and decreased Superoxide dismutase (SOD) and Catalase (CAT) enzyme activities in the I/R group (p??0.001). Degeneration, necrosis, inflammation, loss of striation, interfibrillar and interfascicular edema were seen in the histopathological examination of the skeletal muscles in the I/R group. These histopathological findings were minimal in the I/R+AST group. In the immunohistochemical examination of muscle tissue with the GPx1 primary antibody, a mild degree of GPx1 reactivity was observed in the I/R group, and a moderate degree of GPx1 reactivity was seen in the I/R+AST group. As a result, the strong ameliorative effect of AST on ischemia-reperfusion injury and its complications on skeletal muscles was demonstrated by biochemical, histopathological and immunohistochemical examinations.
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    Investigation of the protective effect of ellagic acid for preventing kidney injury in rats exposed to nicotine during the fetal period
    (Taylor & Francis Ltd, 2016) Akkoyun, H. T.; Karadeniz, A.
    We investigated the possible protective effects of ellagic acid on rat kidneys exposed to nicotine during the fetal period. Twenty pregnant female rats were divided randomly into four groups: control (C), nicotine (N), ellagic acid (EA) and nicotine + ellagic acid (N + EA). Nicotine and ellagic acid treatments were continued throughout the pregnancies and for 15 days after delivery. On day 15, all neonatal pups were sacrificed and their kidneys were removed for biochemical and histopathological examination. The nicotine treatment significantly decreased body weight, total glutathione (GSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, and increased malondialdehyde (MDA) and nitric oxide (NO) levels in the N group compared to controls. EA treatment ameliorated decreased body weight, GSH, GSH-Px and SOD activities, and increased MDA and NO levels in group N + EA compared to group N (p < 0.05). Nicotine caused kidney damage as shown by incomplete development of glomeruli and Bowman's capsules. Nicotine also caused greater apoptosis in group N compared to group C. Ellagic acid treatment produced histological kidney structure that was closer to normal and it exerted an anti-apoptotic effect in the N + EA group compared to the N group. EA played a protective role against nicotine-induced nephrotoxicity and oxidative stress in rats owing to its antioxidant, radical scavenging and anti-apoptotic effects.
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    PROTECTIVE EFFECT OF ASTAXANTHIN IN THE LUNG INJURY CAUSED BY ISCHEMIA REPERFUSION OF THE LOWER EXTREMITIES
    (Pakistan Agricultural Scientists Forum, 2019) Akkoyun, H. T.; Uyar, A.; Bengu, A. S.; Akkoyun, M. Bayramoglu; Arihan, O.; Keles, O. F.
    Pathological and biochemical alterations due to lower extremity (I/R) damage and protective effects of astaxanthine (AST) were investigated. Rats were divided into four groups. GI-Sham group (n=7):Anesthesia without (I/R)(2hours);GII-I/R (n=7) : 2 hours of ischemia and 2 hours of reperfusion under anesthesia; Group III-AST(n=7): Rats were subchronically orally administered for 7 days at 125 mg/kg astaxanthin (AST) and then anesthetized (2hours) without ischemia; GIV-I/R+AST (n=7) : 7 days prior to ischemia rats were subchronically orally administered 125 mg/kg astaxanthin (AST) and then 2 hours of ischemia and reperfusion under anesthesia; Then lung tissues were investigated for MDA,GSH and histopathology. An increase in MDA and a decrease in GSH was observed I/R administered group compared to control. Histopathological evaluations showed intense congestion in pulmonary veins and alveolar septum and partial alveolar macrophage and erythrocyte accumulation and edema was observed in lumens of some bronchioles and alveoli in the second and fourth group compared control. Second group (3.41) damage score had high significance compared to control (p <= 0.001). Fourth group damage score (0.92) was indifferent from control but significantly different from I/R group (p <= 0.001). As a result; The protective effect of AST has been demonstrated by biochemical, histopathological and immunohistochemical effects.