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    EXPLORING IN VITRO CORM INDUCTION POTENTIAL OF CROCUS SATIVUS THROUGH DIRECT ORGANOGENESIS
    (Aloki Applied Ecological Research And Forensic Inst Ltd, 2024) Sharma, M.; Sharma, M.; Sharma, S. K.; Salgotra, R. K.; Al-Ashkar, I; Pandey, S.; Adnan, M.
    Saffron is an economically high-value crop globally that can give farmers a high income. Its sexually sterile nature demands vegetative propagation and quality planting material. Hence, this study was designed to build a rapid and reproducible procedure for the micropropagation of saffron. We have examined the surface sterilization of corms as explant with fungicides, antibiotics, and 0.1% mercuric chloride, resulting in maximum sprouting (91.07%) with minimum contamination and mortality. As the propagation of saffron corm and its germination is a critical factor for its yield, we have checked different phytohormone concentrations for bud corm germinations. We found that BAP is an efficient cytokinin to initiate buds on corms. MS medium with NAA (0.5 mg L-1 ) and BAP (3.0 mg L-1 )led to 97.77% sprouting in an average of 34.67 days. Subculturing of sprouted corms on the increased concentration of BAP enhanced shoot proliferation from the base of the sprouted bud. The MS medium with NAA (0.5 mg L-1 ) and BAP (6.0 mg L-1 ) shows maximum mean number of shoots (12.67). The presence of growth retardants like ABA, PBZ, and CCC inhibited shoot growth and led to corm development. PBZ (1.5 mg L-1 ) significantly affected cormlet induction (80%), with a maximum mean weight of 1.70g. Accumulative sucrose concentration to 90 g L-1 further increased mean weight (2.52 g) and size of cormlets (1.08 cm). Our in vitro propagation protocol can produce many corms for regular supply to farmers.
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    YIELD AND PHYSIOLOGICAL ASSESSMENT OF SESAME (SESAMUM INDICUM L.) VARIETIES IN RESPONSE TO PLANT GROWTH PROMOTERS
    (Aloki Applied Ecological Research And Forensic Inst Ltd, 2024) Sajid, M.; Amjid, M.; Munir, H.; Rauf, S.; Soufan, W.; Adnan, M.; Ratnasekera, D.
    Sesame is one of the important oilseeds across the globe for edible, pharmaceutical and cosmetic purposes. Sesame is low water requiring crop and grows well on marginal land with less inputs. The study aimed to evaluate the growth, yield and oil content of three sesame varieties TS-3, TS-5 and TH-6 in response to various foliar applications of salicylate (50 mgL(-1)), ascorbate (50 mgL(-1)), hydrogen peroxide (40 mu ML-1) and L-Methionine (150 mgL(-1)). Field trial was executed during May 2021 at Agronomic Research Farm, University of Agriculture, Faisalabad by employing factorial design and three replications. Irrigation was applied first at 25 days after sowing and second after 70 days while as the foliar amendments of growth promoters was applied at 50, 60 and 70 days of crop life. From the results of this experiment, it is concluded that, the variety TH-6 is short duration as compared to other varieties. Effect of foliar application showed significant impact on sesame varieties. The best results were recorded under salicylic acid treatments followed by ascorbic acid and L-methionine while as the least response was recorded without foliar application. The number of seeds per pod, harvest index, thousand seed weight, seed yield kgha(-1) and oil contents were recorded maximum under variety TH-6 when exposed to salicylic acid treatment while as the minimum response was showed in variety TS-3. Moreover, the highest phenolic content was determined by employing ascorbic acid (50 mgL(-1)) followed by salicylic acid (50 mgL(-1)) and the lowest findings were recorded where without foliar application. However, the emerged proline content was assessed in all sesame varieties with control treatment whereas the least findings were recorded from L-Methionine (150 mgL(-1)) treatment.