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    Öğe
    Effects of Nigella sativa on Plasma Oxidative Stress,and Some Apoptotic Protein Markers in Cerebrum andHippocampus in Pentylenetetrazol-Induced Kindling Rats
    (2020) Üstünova, Savaş; Meral, İsmail; Koçyiğit, Abdurrahim; Kılıç, Aysu; Zeybek, Öykü; Üyüklü, Mehmet; Meydan, Sedat
    Objective: This study was designed for investigating the effectiveness of the Nigella sativa (N. sativa) extract on apoptosis in cerebrum and hippocampus and also plasma oxidative stress, in pentylenetetrazol (PTZ)-induced kindling rats. Materials and Methods: The kindling model was induced by subconvulsive doses (35 mg/kg i.p.) of intraperitoneal PTZ injections in N. sativa treated and non-treated PTZ groups (PTZ and PTZ + NS). The PTZ + NS group were also treated with an extract of N. sativa (10 mg/kg) 2 h before each PTZ injection. The total oxidant and antioxidant status, oxidative stress index, paraoxonase, arylesterase, catalase (CAT) and total thiol levels were analyzed in plasma. Brain derived neurotrophic factor (BDNF), cyclin-B1 and B-cell lymphoma 2 (Bcl-2) expressions were investigated in the cerebrum and hippocampus.Results: PTZ decreased the oxidative stress by increasing the activities of CAT, arylesterase, and paraoxonase. N. sativadecreased activities of arylesterase, paraoxonase, while increasing the CAT. It also brought the decreased BDNF and Bcl-2 expression levels to their normal levels in the cerebrum but not in the hippocampus.Conclusion: N. sativa treatment improved the PTZ induced-impairments in BDNF and Bcl-2 expressions, resulting in a neuronal apoptosis in the cerebrum, without affecting blood oxidative stress.
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    Öğe
    Effects of Thymoquinone On Erythrocyte Aggregation Kinetics: An In Vitro Study
    (2022) Üstünova, Savaş; Meral, İsmail; Üyüklü, Mehmet
    Erythrocyte aggregation is associated with a series of physio-pathological processes in body and according to this, current study was designed to examine how the thymoquinone (TQ) and aggregation kinetics relation changes when the aggregation properties of erythrocyte suspensions are intervened using different approaches, such as changing the suspension environment or erythrocyte surface properties. Blood samples from 40 male Wistar albino rats (three months old weighing 250-300 g) were randomly divided into four groups, as 1) whole blood (control), 2) 1/2 dilution (plasma was diluted with PBS), 3) dextran 500 (1% dextran 500 was dissolved in plasma) and 4) glutaraldehyde. Blood samples were taken from abdominal aorta under anesthesia. The erythrocyte suspensions were incubated at 37°C for one hour in the absence or presence of TQ, with a final concentration of 1 mg/ml. Then, the electrical properties of erythrocyte suspensions were recorded, and the electrical capacitance was monitored using an inductance, capacitance, and resistance meter (LCR meter) at 100 kHz. It was found that TQ incubation decreased the aggregation index in control and dextran groups while increased it in 1/2 dilution and GA groups. It was also found that TQ incubation increased the time constant in whole blood and dextran while decreased it in 1/2 dilution and GA. The TQ can normalize the altered erythrocyte aggregation responses in both ways that is, it decreases the increased aggregation or increases the decreased aggregation. Therefore, it may have very critical role in rouleaux formation-induced circulatory disorders.
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    Öğe
    Immune plasma therapy in COVID-19 infection: the experience of Siirt
    (2021) Özüdoğru, Osman; Gungormez, Cigdem; Üyüklü, Mehmet
    Objective: COVID-19 pandemic which is rapidly spreading in the world affects people in Turkey significantly. Various drug-methods for treatment are being tried and one of these applications is immune plasma transfusion. Materials and Methods: In this study, 400cc immune plasma treatment was evaluated retrospectively to show the effect of treatment applied to 23 patients with COVID-19 diagnosis in Siirt Training and Research Hospital. Results: It was observed that the use of mechanical ventilation was 47.82% before the immune plasma treatment, while this need decreased to 30.43% after 3 days. Nine out of 23 patients who received immune plasma therapy recovered by completing their treatment. Conclusion: Although the immune plasma applied on patients contributes to the recovery of patients, the evidence is yet insufficient. Therefore, more COVID-19 patients need to be observed to document the efficiency of immune plasma transfusion therapy.