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Öğe A Serologic Survey of Dirofilariasis, Leishmaniasis, Ehrlichiosis, and Anaplasmosis in Dogs in Siirt Province(2020) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Şahin, Tekin; İrak, Kıvanç; Bolacalı, Memiş; Biçici, Özlem; Baldaz, VedatCanine vector-borne diseases are caused by a wide range of pathogens transmitted by arthropods.The aim of the present study was to determine the prevalence of Dirofilaria immitis antigen andEhrlichia canis, Leishmania infantum, and Anaplasma phagocytophilum/platys antibodies using aRapid test (Anigen Rapid Caniv-4 Leish, Bionote, South Korea). The study was conducted with atotal of 50 mixed-breed dogs (26 males and 24 females). Anaplasma phagocytophilum/platysantibodies were detected in 5 of the dogs (10%). None of the tested dogs were positive for D.immitis antigen or L. infantum and E.canis antibodies. As a result; it was concluded that protectionand control measures regarding anaplasmosis should be taken.Öğe A SURVEY OF TOXOCARA VITULORUM IN ANATOLIAN WATER BUFFALOES (BUBALIS BUBALIS) IN DIYARBAKIR, TURKEY(Assiut University, Faculty of Veterinary Medicine, 2022) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Kilinç, Özlem Orunç; Ayan, Özge Oktay; Görmez, GülToxocara vitulorum is a pathogenic gastrointestinal nematode found in cattle and buffaloes all over the world, especially in tropical and subtropical regions with humid climates. Buffalo farming is a sector whose value has increased in recent years in Turkey and is intensively performed to obtain various products such as milk, cream, meat, and horns. This study aims to determine the prevalence of T. vitulorum in Anatolian Water Buffaloes in Diyarbakır. Fresh stool samples from animals were placed in individual stool containers. The sex and age of the animal were recorded for each sample collected. The samples were evaluated under the light microscope after the application of the Fulleborn saturated salt solution method and under Scanning Electron Microscope. Positivity was detected in 5 (3.01%) of the 166 samples. Regarding prevalence by age groups, T. vitulorum was detected in 10% of the 0-6 month group, 4.17% of the 6-12 month group, and 0.89% of the older than 12 months group. The prevalence by sex was determined as 3.85% for females and as 1.61% for males. To determine the epidemiology of the disease in the region, it was concluded that larger herds should be studied and serological and molecular methods should be used to confirm and support the findings of microscopic methods. © 2022, Assiut University, Faculty of Veterinary Medicine. All rights reserved.Öğe A Survey on Prevalence of Toxocara vitulorum in Calves in Siirt(2022) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Ayan, Özge OktayToxocara genus has important risk for public health, and is a concern for both human and veterinary medicine. Toxocara vitulorum is the largest nematode for cattle and infests the small intestines of water buffalo, bison, and cattle living in tropical and subtropical regions with humid climates. The objective of the present study is to investigate the prevalence of Toxocara vitulorum in calves in Siirt province of Turkey. This study was carried out in the Siirt province, located in the Southeast Anatolian region. The animal material of the study consisted of a total of 100 calves of ages up to 6 months. Stool samples were collected directly from the rectum using disposable latex gloves, after which they were placed in individual stool containers. Samples brought to the laboratory were stored at +4 °C until analysis. Fülleborne's salty water flotation technique was applied to stool samples. The samples were then evaluated under a light microscope.As a result of the inspections, a positivity rate of 7% (7/100) was detected in the samples examined for Toxocara vitulorum by microscopic method. As a result of this study, the prevalence of the disease in Siirt province was revealed, and considering the losses caused by the infection, it was concluded that the disease should not be neglected. Farmers should be informed about the disease and appropriate treatment options for it.Öğe Bir Erkek Keçide Ürolithiazis Olgusu(2014) İçen, Hasan; Şimşek, Aynur; Servet, Sekin; Çelik, Özgür Yaşar; Çakmak, Fırat; Yaman, TuranÇalışmanın materyalini 2-3 gündür idrar yapamama, sancı ve iştahsızlık şikayeti ile Dicle Üniversitesi Veteriner Fakültesi İç Hastalıkları Kliniği’ne getirilen 1,5-2 yaşlı bir erkek keçi oluşturdu. Hastanın klinik muayenesinde ayakta durmakta güçlük, abdominal gerginlik, anal bölge ile prepisyum çevresinde ödem, prepisyumun uç kısmında kuruluk ve küçük taşlar saptandı. Nabız frekansı 119/dk, solunum frekansı 32/dk ve vücut ısı 36.9 °C olarak tespit edilen hayvana, kan ve idrar örneklerinin laboratuar analiz sonuçları ile otopsi bulgularına göre ürolithiazis tanısı konuldu.Öğe Boer-Kıl Keçisi Melezi Bir Keçide Corynebacterium Pseudotuberculosis’in Neden Olduğu Lenfadenitis Olgusu(2016) Gülaydın, Özgül; Akgül, Gülşah; Çelik, Özgür Yaşar; İrak, Kıvanç; Gülaydın, Ali; Gürtürk, Kemal…Öğe Diyarbakır Yöresindeki Sığırlarda Enzotik Bovine Leukosis (EBL), Bovine Viral Diare (BVD), Mavi Dil (BT) ve Infeksiyöz Bovine Rhinotracheitis (IBR) Enfeksiyonlarının Seroprevalansının Araştırılması(2015) Şimşek, Aynur; Gürçay, Metin; Parmaksız, Ayşe; İçen, Hasan; Sekin, Servet; Koçhan, Akın; Çelik, Özgür YaşarSığırlarda viral etkenlerin yol açtığı hastalıklar, verim kaybı ve tedavi giderleri nedeniyle önemli ekonomik kayıplara yol açmaktadır. EBL, BVD, IBR ve BT sığırların görülen önemli viral hastalıklarındandır. Bu çalışmada, solunum sistemi ve sindirim sistemi problemi gibi çeşitli nedenlerle hasta oldukları tespit edilen hayvanlarda EBL, BVD, IBR ve BT hastalıklarının varlığının araştırılması amaçlanmıştır. Çalışmanın materyalini Diyarbakır ilinde halk elinde yetiştirilen ve belirtilen hastalıklara karşı aşılanmamış 1 yaş üstü farklı ırk ve cinsiyette 109 sığırdan alınan kanlardan elde edilen serumlar oluşturdu. Kan serumlarında BVDV Antijen tespiti için, virüsün Erns proteinini bulma temelli ticari BVDV Antijen ELISA test kit (IDEXX BVDV Ag/Serum Plus, Switzerland AG) kullanıldı. BVDV antikorlarının tespiti, ticari BVDV Ab ELISA test kit (IDEXX BVDV Total Ab ELISA, Switzerland) ile yapıldı. Enfeksiyoz Bovine Leucosis (EBL) antikorlarının tespiti için ticari EBL Ab ELISA kit (Pourquier ELISA Leukosis serum screening/BLV Serum-S, Institut Pourquier, France) kullanıldı. IBR antikorlarının tespiti için, ticari Infectious Bovine Rhinotracheitis Virus (EHV-1)g B Antibody test kit (IDEXX, Switzerland AG) kullanıldı. BTV, VP7 proteinine karşı oluşmuş antikorları ortaya koymak için hazırlanmış BTV Antibody test kit (IDEXX Bluetongue Competition, France) kullanıldı. Testler üretici firmaların bildirdikleri prosedürlere uygun olarak yapıldı. Diyarbakır ilinde sindirim (iştahsızlık, ishal, kilo kaybı) ve solunum sistemi (öksürük, burun akıntısı) problemi olan 109 sığıra ait kan serumu örneklerinden 2 tanesinde EBL, 1 tanesinde BVD Ag, 78 tanesinde BVD Ab, 19 tanesinde BT ve 52 tanesinde ise IBR virüsüne karşı antikor belirlendi. Bu hayvanların 17’si (%15.59) kontrol edilen tüm virüsler yönünden seronegatif bulunurken, seropozitif hayvanların 42’si (%38.53) yalnızca bir virüse karşı, 38’i (%34.86) iki virüse karşı ve 11’i (%10.09) de üç virüse karşı pozitif olarak tespit edildi. Dört virüse karşı pozitif hayvan saptanamadı. Sonuç olarak; Diyarbakır bölgesindeki sığırlarda bu enfeksiyonların yaygın olduğu kanaatine varıldı.Öğe Diyarbakır’ın Dicle ve Hani İlçelerindeki Köpeklerde Leishmaniasis’in Klinik, Hematolojik ve Biyokimyasal Bulguları, Serolojik Tanısı ve PCR ile Tiplendirilmesi(2015) Çelik, Özgür Yaşar; Sekin, ServetLeishmaniasis, halk arasında tatarcık olarak bilinen Phlebotominae sineklerinin kan emerken bulaştırdıkları, Leishmania spp. tarafından meydana getirilen insan ve hayvanlarda ölümcül olabilen paraziter bir hastalıktır. Ülkemizde Visseral leishmaniasis olguları başta Ege ve Akdeniz bölgeleri olmak üzere bütün bölgelerimizde görülmektedir. Akdeniz ülkelerinde yapılan çalışmalarda köpeklerde Visseral leishmaniasisin oldukça yaygın olduğu ve köpeklerin L.infantum için rezervuar oldukları bildirilmektedir. Bu çalışma Mayıs-Haziran 2013 tarihleri arasında Diyarbakır’ın Dicle (Dede ve Durabeyli köyleri) ve Hani (sergen ve çardaklı köyleri) ilçelerindeki sahipli köpeklerde canine leishmaniasis seroprevalansının araştırılması amacıyla yapılmıştır. Tekniğine uygun olarak 120 köpeğin vena cephalica antebrachii’lerinden EDTA’lı ve EDTA’sız tüplere kan örneği alındı. Alınan örnekler Rapid test, IFAT, PCR ile incelendi ve köpeklerin tamamı leishmaniasis yönünden negatif bulundu. Benzer çalışmaların bölgedeki sahipsiz köpekleri de kapsayacak şekilde yapılmasının daha iyi sonuçlar doğuracağı kanaatindeyiz.Öğe Effect of Sex on Some Biochemical and Hematological Parameters in Healthy Boer x Hair Goat Crossbreed(2019) Çelik, Özgür Yaşar; İrak, Kıvanç; Akgül, GülşahThe aim of this study was to determine some hematological and biochemical parameters in male and female Boerx hair goat crossbreed (n=34). Blood samples were collected from the jugular vein in sample tubes with andwithout anticoagulants. A Mindray BC2800 fully-automated device was used to measure the WBC, RBC, Hgb,Hct, MCV, and MCH values and ADVIA 1800 brand auto-analyzer was used to obtain Na, K, Cl, Mg, Ca, Crea,Tp, Alb, Gluo, Chol, Trig, AST, ALT, and ALP values. WBC (p<0.01), RBC (p<0.05), HCT (p<0.01) and MCV(p<0.05) levels were found to be statistically higher in female specimens, while HGB and MCH levels were foundto be statistically identical for both sexes. Meanwhile, males had statistically higher levels of K (p <0.001) and Mg(p<0.01) levels compared to females. Crea (p<0.001), Tp (p <0.05), Alb (p<0.05), Gluo (p<0.001) levels werealso statistically higher in males, while Chol level was statistically higher in females (p<0.001). No statisticallysignificant variation was detected between the Trig values of the sexes. ALT, ALP activities of males were foundto be statistically higher in males than in females (p<0.05). The results of the study revealed that somehematological and biochemical parameters of Boer x hair goat crossbreeds are affected by sex.Öğe Investigation of Seroprevalence of Bluetongue Diseases in Sheep in the Province of Siirt(2019) Çelik, Özgür Yaşar; Şahin, TekinBluetongue is a viral infection that is commonly encountered in domestic and wild ruminants and is transmitted by biting midges. Thecause of the disease is within Orbivirus genus of Reoviridae family and has 24 known serotypes. The infection is spread by the flies ofCulicoides species. The aim of this study was to determine the serological presence of the bluetongue infection in sheep raised invarious locations of Siirt province and to obtain information about the spread of the infection in the region as a whole. The study wasconducted between August 2017 and February 2018, and the study consisted of 465 sheep (1-3 years old). 5 mL blood samples weretaken from the jugular veins of the sheep to non-anticoagulant tubes for laboratory analyses. The blood samples were centrifuged at3000 rpm/10 minutes and the serum were transferred to Eppendorf tubes, which were stored at -20 oC until analysis was carried out.Determination of the Bluetongue-specific antibodies in the serum samples was performed using a commercial test kit by ELISA device.The results of the specific-antibody analysis revealed that 340 (73.12%) of 465 sheep were seropositive, while 125 (26.88%) wereseronegative. As a result, the seroprevalence of Bluetongue disease was found to be considerably high in various locations of Siirtprovince. Considering the findings of the study, in addition to the measures like the establishment of quarantine protocols and vectorcontrols, utilization of different diagnostic methods and use of vaccines specific to the virus serotypes are suggested to fight theinfection.Öğe Investigation of Seroprevalence of Maedi-Visna and Caprine Arthritis Encephalitis in Sheep and Goats in Siirt Province(2018) Çelik, Özgür Yaşar; Akgül, Gülşah Demir; İrak, KıvançMaedi-visna (MV) and Caprine Arthritis-Encephalitis (CAE) are economically important viral diseases that infectsheep and goats. The aim of this study is to determine the seroprevalence of MVV and CAEV on sheep and goats raised inSiirt region by using the ELISA method. Blood samples were collected to non-anticoagulant tubes from the jugular veins of apopulation of 465 small ruminants consisting of 182 sheep and 283 goats selected from different districts of Siirt Province.The samples were centrifuged for 10 minutes at 3000 rpm and the serum were transferred to Eppendorf tubes, which werestored at -20°C until further analysis. Commercial kits (IDEXX MVV / CAEV p28 Ab screening, IDEXX, USA) were used for thedetection of Anti-MVV/CAEV antibodies. All samples were found to be seronegative in terms of Anti-MVV/CAEV antibodies.As a result of this study conducted in the Siirt province, MVV and CAEV infections were not detected in the studied population.Given that the infections exist with varying seroprevalence levels in different regions of Turkey, however, it might beworthwhile to investigate the infection status in Siirt province by including larger herds in future studies.Öğe Investigation of Seroprevalence of Toxoplasma gondii in Sheep and Goats in Siirt Province in Turkey(2020) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Mor, Neriman; İrak, KıvançThis study was carried out to determine the seroprevalence of toxoplasmosis in sheep and goats in Siirt province. Blood samples werecollected from a total of 450 female animals, consisting of 200 goats and 250 sheep in different part of Siirt province. Samples were centrifugedat 3000 rpm for 10 minutes and stored at -20 oC until use. The study was carried out by ELISA method using commercial test kit (Toxoplasmagondii Antibody Test Kit, IDEXX). According to the results of this study 28 of the 450 animals (6.22%) were positive, 393 (87.33%) werenegative, 17 (3.78%) were suspicious, while 12 (2.67%) were weak positive. As the result of the analyzes performed using the chi-square testa significant difference was found between the results and the species (p<005), and no significant difference between the results and theages group of the animals was observed (p>0.05). As a result, the presence of T. gondii in sheep and goats in Siirt region was determined forthe first time by this study. Since this study provides information about the current situation of T. gondii infection in Siirt region, it is thoughtthat it will contribute to taking necessary measures in terms of public health and disease control practices.Öğe Kedi ve Köpeklerde Kardiyovasküler Hastalıkların Tanısında Natriüretik Peptidler’in Önemi(2009) İçen, Hasan; Çelik, Özgür Yaşar; Şimşek, AynurKalp yetmezliği ile ilgili hastalıkların patofizyolojisinin anlaşılması ve sağaltımı hakkında son yıllarda oldukça fazla ilerlemeler kaydedilmiştir. Hastalığın tanısında detaylı fiziksel muayeneler yapılmasına rağmen kedi ve köpeklerde yine de zorluklarla karşılaşılmaktadır. Kalp hastalıklarının klinik tanısında; anamnez, fiziksel muayene, kardiyopulmoner oskültasyon ve göğüs radyografisinden yararlanılmaktadır. Tanıda daha çok anlam ifade eden ekokardiyografi kullanımı ve uygulaması uzmanlık gerektirmekte ve aynı zamanda hayvan sahibine ek bir maliyet yüklemektedir. Bu nedenle serum biyomarkırları kalp hastalıklarının tanısı ve sağaltıma verilen cevabın belirlenmesinde kullanılmaktadır. Natriüretik peptidler kalp hastalıklarının tanısında önemli bir yer tutmaktadırlar. Natriüretik peptidler; natriürezisi, idrar üretimini ve böbrek kan akımını arttırırken, sistemik damar direncini ve kalpte dolum basıncını azaltarak diyastololik fonksiyonu etkilemektedir. Kalp hastalıklarının tanısı belirlemede önemli bir protein olmasından dolayı natriüretik peptidlerin kullanımı gittikçe yaygınlaşmaktadır. Bu derlemenin amacı; kalp hastalıklarının tanısında natriüretik peptidlerin etkinliğinin irdelenmesi amaçlanmıştır.Öğe Molecular Investigation and Phylogenetic Analysis of Ehrlichia canis in Dogs in Siirt, Türkiye(2022) Çelik, Burçak Aslan; Yılmaz, Ali Bilgin; Çelik, Özgür Yaşar; Ayan, Adnan; Kılınç, Özlem Orunç; Özdemir, Ramazan; Ayan, Özge OktayEhrlichia canis is the primary etiologic agent of canine monocytic ehrlichiosis, a tick-transmitted disease of dogs. The aim of this study is to molecularly investigate the presence of E. canis and to reveal its prevalence in dogs in Siirt province. The animal material of the study is consisted of a total of 82 dogs. A region of the 16S ribosomal RNA gene of E. canis was targeted for PCR amplification. As a result of the conducted Nested-PCR, positivity was detected at the rate of 10.53% (4/38) in male dogs and 13.64% (6/44) in females, and Ehrlichia canis specific bands of size 389 bp were obtained in 10 (12.20%) dogs in total. The phylogenetic tree was constructed with the Maximum Likelihood (MCL) method, The nucleotide sequence was registered in the NCBI GenBank database with access numbers OK331365.1-OK331366. Early detection of the disease by means of hematological, serological, or molecular tests is very important in terms of prognosis. More studies should be performed to determine vector-disease relationships in this region about ticks that vector the disease.Öğe MOLECULAR INVESTIGATION OF SOME BACTERIA (COXİELLA BURNETİİ, MYCOPLASMA HAEMOCANİS, CANDİDATUS MYCOPLASMA HAEMATOPARVUM, WOLBACHİA) IN RHIPICEPHALUS SANGUINEUS TICKS IN SIIRT PROVINCE, TURKEY(Assiut University, Faculty of Veterinary Medicine, 2022) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Yilmaz, Ali Bilgin; Kilinç, Özlem Orunç; Şababoğlu, Ezgi; Ayan, Özge OktayTicks harbor the largest diversity of microorganisms, ranging from viruses, prokaryotes, and eukaryotes. Rhipicephalus sanguineus ticks are the most common ticks worldwide. Although dogs are the main host of this tick species, it has been reported that it also infests humans in various parts of the world. This study aimed to examine some bacteria (Coxiella burnetii, Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Wolbachia) in Rhipicephalus sanguineus ticks sampled from dogs. In this study, 350 tick samples collected from 85 dogs in Siirt province were determined to be Rhipicephalus sanguineus ticks. Coxiella DNA was detected in 3 (0.85%) out of 350 ticks using Nested PCR (687 base pairs). None of the samples were found to contain Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, and Wolbachia DNA. A partial sequence of the IS1111 gene region was registered in GenBank with OM472143 accession numbers. Considering the zoonotic nature of the Q disease, it is very important for dog owners and related institutions to periodically spray animals against ticks, and to take any other necessary precautions. More samples are needed to determine the Mhc, CMhp, and Wolbachia prevalence. © 2022, Assiut University, Faculty of Veterinary Medicine. All rights reserved.Öğe Molecular Survey of the Toxoplasma gondii and Neospora caninum in brain tissue of aborted fetuses of Morkaraman sheep in Muş, Türkiye(2023) Koca, Davut; Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Ayan, Adnan; Kılınç, Özlem Orunç; Turgut, Ali Osman; Ayan, Özge OktayToxoplasma gondii and Neospora caninum are obligate intracellular protozoan parasites that can affect different warm-blooded species worldwide. In this study, it was aimed to detect T. gondii and N. caninum using PCR method in brain tissues of aborted sheep fetuses. Brain specimens were collected from 50 Morkaraman sheep fetuses that had undergone abortion at various stages of pregnancy, within the lambing seasons of 2023 in Muş. Approximately 1 cm³ of brain tissue from the right cerebral hemisphere was excised and subsequently frozen at -20°C for DNA extraction. DNA extraction and PCR amplification were then performed. As a result of this study, 11 (22%) of 50 brain tissues were positive. All brain samples examined in this study were negative for Neospora caninum. Based on the results of this study, it is possible to say that T. gondii is an important abortion agent in sheep in this region. Although N. caninum was not detected in this study, larger scale studies are recommended. Moreover, this study provides important information to breeders and veterinarians in the evaluation and management of abortion in the field.Öğe Retrospective Evaluation of Canine Leishmaniasis in Turkey(2019) Çelik, Burçak Aslan; Çelik, Özgür Yaşar; Şahin, TekinLeishmaniasis is a zoonotic parasitic disease that is found in parts of the tropics, subtropics, andsouthern Europe. Due to its zoonotic nature, it is as a very important problem for both animal andhuman health, and it poses a significant risk for approximately 310 million people in 98 countries.Like many protozoan parasites, Leishmania have a digenetic life cycle involving both a mammalianhost and an insect vector. The disease has at least four main clinical forms, namely the visceral,cutaneous, mucocutaneous, and diffuse cutaneous forms. In Turkey, two forms of the diseasecaused by various Leishmania species can be encountered: the cutaneous leishmaniasis andvisceral leishmaniasis. Domestic dogs in urban and peri-urban areas and wild canids in the ruralareas or nature are the main reservoirs for visceral leishmaniasis. Dogs are important for thespread of the disease as they can both clinically be infected and can act as reservoirs for othermammals, particularly humans. Studies performed in Turkey indicate that the prevalence of thedisease is between 0% and 27.18%. The prevalence of the disease for the Mediterranean, Aegean,Central Anatolia, Black Sea, Eastern Anatolia, Marmara, and Southeastern Anatolia regions havebeen determined as 12.96%, 9.08%, 5.82%, 5.38%, 4.38%, 2.40%, and 0.00%, respectively. Inthis review, the aim was to retrospectively evaluate the prevalence of the canine leishmaniasis inTurkey.Öğe Veteriner Hekimlikte Kan Transfüzyonu(2018) Çelik, Özgür Yaşar; İçen, Hasan; Şimşek, Aynur; Koçhan, AkınKan her biri ayrı fonksiyona sahip son derece spesifik yapılardan oluşmuş bir bütün, canlı bir dokudur. Kan ürünleri denilince aklahem kan komponentleri hem de plazma fraksiyon ürünleri gelmelidir. Son 25 yılda veteriner hekimlikte hasta veya yaralı hayvanlarayapılan kan nakli nispeten nadir bir olaydan rutin ve sıklıkla hayat kurtaran bir uygulamaya dönüşmüştür. Her ne kadar komponenttedavisi veteriner hekimlikte henüz yeterince yaygınlaşmamış olsa da son yıllarda kan bileşenleri tedavisi hakkında bilgiyaygınlaştıkça veteriner hekimlerde artık kan nakillerinde sadece tam kan değil kan komponentleri kullanmanın öneminin farkınavarmıştır. Veteriner hekimlikte eritrosit replasmanı, hemostatik protein replasmanı, non-hemostatik protein replasmanı ve trombositreplasmanı gereken durumlar için kan transfüzyonu yapılabilir. Transfüzyonda endikasyona göre taze tam kan, saklanmış tam kan,taze donmuş plazma, plazma, kriopresipitat, kriopresipitatdan fakir plazma, trombositten zengin plazma gibi kan komponentlerikullanılabilmektedir.