Temel, YusufBengu, Aydin SukruAkkoyun, Hurrem TuranAkkoyun, MahireCiftci, Mehmet2024-12-242024-12-2420171095-66701099-0461https://doi.org/10.1002/jbt.21954https://hdl.handle.net/20.500.12604/5883In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2,5-ADP-Sepharose 4B affinity gel. The effects of Ast and Al3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al3+ inhibited the enzyme activity noncompetitively (IC50 values; 0.679mM, K-i values 1.32mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al+Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group (P<0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al+Ast groups (P<0.05).eninfo:eu-repo/semantics/closedAccessaluminumastaxanthinenzyme purificationglucose-6-phosphate dehydrogenase (G6PD)6-phosphogluconate dehydrogenase (6PGD)inhibitionrat erythrocyteArticle3110Q4WOS:000412328200013Q22-s2.0-850217181522866554810.1002/jbt.21954