Durmaz, LokmanKiziltas, HaticeKaragecili, HasanAlwasel, SalehGulcin, Lhami2024-12-242024-12-2420231319-01642213-7475https://doi.org/10.1016/j.jsps.2023.101760https://hdl.handle.net/20.500.12604/6642Onion contains many dietary and bioactive components including phenolics and flavonoids. Spiraeoside (quercetin-4-O-b-D-glucoside) is one of the most putative flavonoids in onion. Several antioxidant techniques were used in this investigation to assess the antioxidant capabilities of spiraeoside, including 1,1diphenyl-2-picrylhydrazyl radical (DPPHGreek ano teleia) scavenging, N,N-dimethyl-p-phenylenediamine radical (DMPD'') scavenging, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS'') scavenging activities, cupric ions (Cu2') reducing and potassium ferric cyanide reduction abilities. In contrast, the water-soluble a-tocopherol analogue trolox and the conventional antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and a-tocopherol were utilized as the standards for evaluation. Spiraeoside scavenged the DPPH radicals an IC50 of 28.51 mu g/mL (r2: 0.9705) meanwhile BHA, BHT, trolox, and a-tocopherol displayed IC50 of 10.10 mu g/mL (r2: 0.9015), 25.95 mu g/mL (r2: 0.9221), 7.059 mu g/mL (r2: 0.9614) and 11.31 mu g/mL (r2: 0.9642), accordingly. The results exhibited that spiraeoside had effects similar to BHT, but less potent than a-tocopherol, trolox and BHA. Also, inhibitory effects of spiraeoside were evaluated toward some metabolic enzymes including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II) and a-glycosidase, which are related to a number of illnesses, such as Alzheimer's disease (AD), diabetes mellitus and glaucoma disorder. Spiraeoside exhibited IC50 values of 4.44 nM (r2: 0.9610), 7.88 nM (r2: 0.9784), 19.42 nM (r2: 0.9673) and 29.17 mM (r2: 0.9209), respectively against these enzymes. Enzyme inhibition abilities were compared to clinical used inhibitors including acetazolamide (for CA II), tacrine (for AChE and BChE) and acarbose (for a-glycosidase). Spiraeoside demonstrated effective antioxidant, anticholinergic, antidiabetic and antiglaucoma activities. With these properties, it has shown that Spiraeoside has the potential to be a medicine for some metabolic diseases. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).eninfo:eu-repo/semantics/openAccessSpiraeosidea-glycosidaseEnzyme inhibitionCarbonic anhydraseAntioxidant activityAcetylcholinesteraseArticle3110Q2WOS:001078411700001Q22-s2.0-851698189913769373510.1016/j.jsps.2023.101760